The expression of erythropoietin triggered by Danggui Buxue Tang, a Chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis, is mediated by the hypoxia-inducible factor in cultured HEK293T cells

Zheng, Yuzhong; Choi, Roy Chi Yan; Xie, Heidi Qunhui; Cheung, Anna Wing Han; Guo, Ava J.Y.; Leung, Ka Wing; Chen, Vicky P.; Bi, Cathy W. C.; Zhu, Kevin Y.; Chan, Gallant Kar-Lun; Fu, Qiang; Lau, David Tai-Wai; Dong, Tina T. X.; Zhao, Kui; Tsim, Karl Wah Keung

doi:10.1016/j.jep.2010.08.029

Cited by 67 publications

(67 citation statements)

References 31 publications

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“…26) Following the transfection of fibroblast kidney cells (HEK293T) with a plasmid containing a hypoxia response element (HRE), DBT triggers the expression of hypoxia-inducible factor-1α (HIF-1α) and EPO, which is relevant to the signaling pathway of mitogen-activated protein kinases. 27) Our research also noted that Astragalus alone slightly up-regulated the level of EPO but did not have a distinct effect on GM-CSF or TPO; GM-CSF, TPO and EPO were not affected by Angelica alone. These results demonstrated that the effect of Astragalus or Angelica alone on HGF was slight.…”

Section: Discussionsupporting

confidence: 58%

Effects of Astragalus Combined with Angelica on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

Tang

Chen

et al. 2017

Biological & Pharmaceutical Bulletin

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Danggui Buxue Tang (DBT), a combination of Astragalus and Angelica at a 5 : 1 ratio, mainly promotes hematopoiesis. However, in the clinic, the combination ratio of Astragalus and Angelica to treat low hematopoietic function is not an absolute 5 : 1 ratio, suggesting that the herbs may promote hematopoiesis better after being combined at a certain range of ratios. The objective of this study is to investigate the effect of different ratio combinations of Astragalus and Angelica on bone marrow hematopoiesis suppression induced by cyclophosphamide (CTX) and to probe the interaction and mechanism of Astragalus combined with Angelica in promoting hematopoiesis. Following establishment of the model, mice were administered with Astragalus (6.00 g·kg 1

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Section: Discussionsupporting

confidence: 58%

Effects of Astragalus Combined with Angelica on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

Tang

Chen

et al. 2017

Biological & Pharmaceutical Bulletin

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“…For the past decades, our group has studied several decoctions with Astragalius Radix, such as Danggui Buxue Tang [16] [17] [18]; Fo Shou San and Yu Ping Feng San [19] [20] [21]. Astragalius Radix was able to abolish the ubiquity in degradation system and resulted the accumulation of hypoxia inducible factor 1 alpha (HIF-1α) [22] [23]. Thus, a pseudo-hypoxic environment could be generated at cellular level.…”

Section: Resultsmentioning

confidence: 99%

A High Throughput Screening Platform for Skin Tuning Properties from Natural Products: Identification of Skin Tanning Compounds

Chan¹,

Wu²,

Wong³

et al. 2016

JCDSA

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Skin lightening and tanning are two major areas dominating the market of skincare and cosmetic products. Though the demands are originated from two different communities, the two areas share a same goal-skin colour tuning. The known safe compounds with skin colour tuning activities are limited. In contrary, Chinese medicinal herb provides a pool of natural bioactive compounds, which have been used in Asian countries for long time and have been tested for its toxicity. Here, we demonstrate a high throughput screening platform for potential compounds usable for skin colour tuning. From 147 natural compounds, 26 of them showed potential in skin tanning functions by using the high throughput melanogenesis platform based on the melanogenesis assay on B16 melanocytes. Five of them promoted melanogenesis by over 50%. Moreover, apart from 1, 8-dihydroxyanthraquinone, the other four compounds showed enhancement effect on tyrosinase activity. From the result, the compounds increased the Vmax of tyrosinase without changing the Km in a dosedependent manner. Thus, there should be no irreversible structural change of the enzyme. Definitely, this report contributes to the development of personalization in skincare and cosmetic products.

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“…Though DBT exhibited potential estrogenic effects in vitro [5, 6, 8], the information on potential hormonal activities of DBT in vivo is limited. It is well known that proliferation of endometrial cells is under the control of estrogens and that the risk of endometrial carcinoma increases with estrogen replacement therapy [15].…”

Section: Discussionmentioning

confidence: 99%

“…Two chemical markers in AR (calycosin and formononetin) and two others in ASR (ferulic acid and ligustilide) were used to standardize the chemical property of DBT. The standardized DBT should contain no less than 0.186 mg calycosin, 0.155 mg formononetin, 0.351 mg ferulic acid, and 0.204 mg ligustilide per one g dried weight of DBT, as reported previously [5, 6, 8]. …”

Section: Methodsmentioning

confidence: 99%

“…TCM, containing thousands of medicinal herbs, is a promising resource that could provide a perfect solution [4]. Indeed, some of the herbal products showed a pronounced efficacy for menopausal symptoms and as a consequence were widely used by women to relieve their menopausal symptoms, for example, DBT [5–8]. In vitro experiments with mammary gland [6] and bone [9] derived cells suggested that at least some of the effectiveness of DBT is mediated through estrogen receptor- (ER-) dependent mechanisms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, in Uterus and Liver are Both Estrogen Receptor‐Dependent and ‐Independent

Zierau

Zheng

Papke

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Danggui Buxue Tang (DBT), a herbal decoction containing Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), has been used in treating menopausal irregularity in women for more than 800 years in China. Pharmacological results showed that DBT exhibited significant estrogenic properties in vitro, which therefore suggested that DBT could activate the nuclear estrogen receptors. Here, we assessed the estrogenic properties of DBT in an ovariectomized in vivo rat model: DBT was applied to the ovariectomized rats for 3 days. The application of DBT did not alter the weight of uterus and liver, as well as the transcript expression of the proliferation markers including the estrogen receptors α and β. However, DBT stimulated the transcript expression of the estrogen responsive genes. In addition, the inductive role of DBT on the expression of members of the aryl hydrocarbon receptor family in uterus and liver of ovariectomized rats was confirmed. These responses of DBT however were clearly distinct from the response pattern detectable here for 17β-estradiol. Therefore, DBT exhibited weak, but significant, estrogenic properties in vivo; however, some of its activities were independent of the estrogen receptor. Thus, DBT could be an exciting Chinese herbal decoction for an alternative treatment of hormone replacement therapy for women in menopause without subsequent estrogenic side effects.

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The expression of erythropoietin triggered by Danggui Buxue Tang, a Chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis, is mediated by the hypoxia-inducible factor in cultured HEK293T cells

Cited by 67 publications

References 31 publications

Effects of <i>Astragalus</i> Combined with <i>Angelica</i> on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

Effects of <i>Astragalus</i> Combined with <i>Angelica</i> on Bone Marrow Hematopoiesis Suppression Induced by Cyclophosphamide in Mice

A High Throughput Screening Platform for Skin Tuning Properties from Natural Products: Identification of Skin Tanning Compounds

Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, in Uterus and Liver are Both Estrogen Receptor‐Dependent and ‐Independent

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