The expression of epidermal growth factor receptors and their ligands (epidermal growth factor, neuregulin, amphiregulin) in the bitch uterus during the estrus cycle

Sağsöz, Hakan; Lïman, Narin; Saruhan, Berna Güney; Küçükaslan, İbrahim

doi:10.1016/j.anireprosci.2014.04.005

Cited by 7 publications

(4 citation statements)

References 41 publications

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“…In turn, a wide range of other neuronal factors observed in NRG-1-LI nerves may indicate multidirectional roles of this substance. However, in spite of the fact that the participation of NRG-1 in the control of maturation and regulation of the estrus cycle have been confirmed [ 15 ], the exact roles of these substance in neuronal control of the reproductive system are pure conjecture. It can be concluded that these roles are similar to those which have been investigated in other parts of the nervous system.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that in the ovary, the described substance is involved in regulatory processes connected with the survival and differentiation of granulosa cells, as well as oocyte maturation [ 13 ]. In turn, within the uterus NRG-1 plays roles in endometrium maturation [ 14 ] and estrus cycle regulation [ 15 ]. Furthermore, important functions of NRG-1 during pregnancy have been confirmed.…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

Rytel

2018

IJMS

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Bisphenol A (BPA), a substance commonly used in the manufacture of plastics, shows multidirectional negative effects on humans and animals. Due to similarities to estrogens, BPA initially leads to disorders in the reproductive system. On the other hand, it is known that neuregulin 1 (NRG-1) is an active substance which enhances the survivability of cells, inhibits apoptosis, and protects tissues against damaging factors. Because the influence of BPA on the nervous system has also been described, the aim of the present study was to investigate for the first time the influence of various doses of BPA on neuregulin 1-like immunoreactive (NRG-1-LI) nerves located in the porcine uterus using the routine single- and double-immunofluorescence technique. The obtained results have shown that BPA increases the number and affects the neurochemical characterization of NRG-1-LI in the uterus, and changes are visible even under the impact of small doses of this toxin. The character of observed changes depended on the dose of BPA and the part of the uterus studied. These observations suggest that NRG-1 in nerves supplying the uterus may play roles in adaptive and protective mechanisms under the impact of BPA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

Rytel

2018

IJMS

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“…Thus, by interacting with the epidermal growth factor (EGF)-related pathways, ECM1 promotes cellular differentiation and proliferation (Lee et al 2014). Within the canine uterus, the localization patterns of EGF receptor (EGFR) were described previously (Sagsoz et al 2014) and reflect the localization of ECM1 presented herein. A possible functional relationship between these two entities is certainly worth attention and should be addressed in the future.…”

Section: Structural and Adhesive Proteins Of The Ecmmentioning

confidence: 54%

Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog

et al. 2018

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For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine () pregnancy. However, previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Here, the expression of selected genes involved in structural functions, cell-to-cell communication and inhibition of matrix metalloproteinases were targeted utilizing qPCR and immunohistochemistry. We found that canine free-floating embryos affect gene expression of , and This seems to be associated with modulation of trophoblast invasion, and proliferative and adhesive functions of the uterus. Although not modulated at the beginning of pregnancy, the decrease of structural ECM components (i.e., , and ) from pre-implantation toward post-implantation at placentation sites appears to be associated with softening of the tissue in preparation for trophoblast invasion. The further decrease of these components at placentation sites at the time of prepartum luteolysis seems to be associated with preparation for the release of fetal membranes. Reflecting a high degree of communication, intercellular cell adhesion molecules are induced following placentation () or increase gradually toward prepartum luteolysis (). The spatio-temporal expression of TIMPs suggests their active involvement in modulating fetal invasiveness, and together with , they appear to protect deeper endometrial structures from trophoblast invasion. With this, the dog appears to be an interesting model for investigating placental functions in other species, e.g. in humans in which appears to share several similarities with canine subinvolution of placental sites (SIPS). In summary, the canine uterine ECM is only moderately modified in early pregnancy, but undergoes vigorous reorganization processes in the uterus and placenta following implantation.

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“…Estradiol (E 2 ) and progesterone (P 4 ) regulate the expression of a wide variety of growth factors and cytokines playing an important role in uterine receptivity, embryonic growth and implantation (Brooks et al, 2014). Also, epidermal growth factor (EGF) and its receptor (EGFR), nodal growth differentiation factor (NODAL) and prostaglandin-endoperoxide synthase 2 (PTGS2) play a key Studies suggested that their altered expression have been related to subfertility in different species (Park & Dufort, 2013;Sagsoz et al, 2014;Wagener et al, 2017), but there is limited information about its endometrial mRNA expression in RBC. Therefore, an altered uterine environment, produced by changes in the endometrial mRNA expression, may be responsible for subfertility in RBC.…”

Section: Introductionmentioning

confidence: 99%

Endometrial expression of key genes related to fertility in repeat breeder and non‐repeat breeder cows

Jaureguiberry

Madoz

Quintana

et al. 2020

Reprod Domestic Animals

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The aim of the present study was to compare the endometrial gene expression of epidermal growth factor receptor (EGFR), nodal growth differentiation factor (NODAL), prostaglandin-endoperoxide synthase 2 (PTGS2), oestrogen receptor 1 (ESR1) and progesterone receptor (PGR) in repeat breeder cows (RBC) and non-RBC during diestrus. Endometrial samples were collected by cytobrush technique and stored in RNA stabilizing solution at −20°C until RT-qPCR analysis. Differences in endometrial mRNA expression of selected genes were assessed by ANOVA and simple (r) and the partial correlations (rp) among selected genes were performed. Results demonstrated that mRNA expression of EGFR and NODAL were higher in RBC than in non-RBC (3 and 25-fold change, p < .01 and p < .01, respectively), while the mRNA expression of PTGS2 was lower (1.56-fold change, p < .01). Although there were no differences detected in the mRNA expression of ESR1 and PGR, there was a positive correlation between the expression of ESR1 and EGFR (0.84, p < .05) and a negative correlation between PGR and PTGS2 (−0.49, p < .05). In conclusion, the difference on the endometrial mRNA expression of the genes included in the study between RBC and non-RBC indicates a deregulation of important mechanisms that are vital to establish a successful pregnancy. Thus, the present study provides useful insight as a base for future studies to elucidate the causes of RBC.

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The expression of epidermal growth factor receptors and their ligands (epidermal growth factor, neuregulin, amphiregulin) in the bitch uterus during the estrus cycle

Cited by 7 publications

References 41 publications

The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

The Influence of Bisphenol A (BPA) on Neuregulin 1-Like Immunoreactive Nerve Fibers in the Wall of Porcine Uterus

Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog

Endometrial expression of key genes related to fertility in repeat breeder and non‐repeat breeder cows

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