Endometrial expression of key genes related to fertility in repeat breeder and non‐repeat breeder cows

Jaureguiberry, María; Madoz, Laura Vanina; Quintana, Silvina; Marin, Maia Solange; Burucúa, Mercedes; Tizzano, Marco Antonio; Rabaglino, María Belén; Rearte, Ramiro; Pecoraro, Marcelo Ítalo; Sota, Rodolfo Luzbel de la

doi:10.1111/rda.13841

Cited by 7 publications

(6 citation statements)

References 21 publications

(34 reference statements)

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“…PPP2R2A has been demonstrated to promote implantation of embryos in vitro and in vivo, respectively, via Ppp2r2a knockout mice and function gain and loss assays of PPP2R2A in sheep ESCs [60,61]. PTGS2 was reported to be more abundant in repeat breeder cows (RBC) than in non-RBC and is involved in the regulation of some protein markers in the in vitro endometrial receptivity of endometrial epithelial cells (EEC) in dairy goats [62,63]. Hiraoka et al reported that the collaborative regulation of uterine receptivity and embryo attachment involves distinct pathways utilized by epithelial and stromal STAT3, resulting in synergistic effects [64].…”

Section: Discussionmentioning

confidence: 99%

CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

Li,

Yao,

et al. 2023

Cells

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The successful establishment of endometrial receptivity is a key factor in ensuring the fertility of ewes and their economic benefits. Hu sheep have attracted attention due to their high fecundity and year-round estrus. In this study, we found that in the luteal phase, the uterine gland density, uterine coefficient, and number of uterine caruncles of high-fertility Hu sheep were higher than those of low-fertility Hu sheep. Thousands of differentially expressed genes were identified in the endometrium of Hu sheep with different fertility potential using RNA sequencing (RNA-Seq). Several genes involved in endometrial receptivity were screened using bioinformatics analysis. The qRT-PCR analysis further revealed the differential expression of cAMP reactive element binding protein-1 (CREB1) in the Hu sheep endometrium during the estrous cycle. Functionally, our results suggested that CREB1 significantly affected the expression level of endometrial receptivity marker genes, promoted cell proliferation by facilitating the transition from the G1 phase to the S phase, and inhibited cell apoptosis and autophagy. Moreover, we observed a negative linear correlation between miR-134-5p and CREB1 in the endometrium. In addition, CREB1 overexpression prevented the negative effect of miR-134-5p on endometrial stromal cell (ESC) growth. Taken together, these data indicated that CREB1 was regulated by miR-134-5p and may promote the establishment of uterine receptivity by regulating the function of ESCs. Moreover, this study provides new theoretical references for identifying candidate genes associated with fertility.

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Section: Discussionmentioning

confidence: 99%

CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

Li,

Yao,

et al. 2023

Cells

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“…Moreover, GAPDH and HPRT1 (along with SDHA ) belonged to the least stably expressed RGs in bovine ovaries 12 . Nonetheless, GAPDH continues to be used as the internal control in many gene expression studies of bovine reproductive tissues 33 – 36 .…”

Section: Discussionmentioning

confidence: 99%

Reference gene selection in bovine caruncular epithelial cells under pregnancy-associated hormones exposure

Sozoniuk

Jamioł

Kankofer

et al. 2022

Sci Rep

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Examination of transcriptional regulation occurring during pregnancy establishment and maintenance requires the identification of endogenous reference genes characterized by high expression stability. Since the expression of some reference genes may be modulated by pregnancy-associated hormones, the goal of our study was to identify suitable reference genes unaffected by hormonal treatment. In our study bovine caruncular epithelial cells were subjected to progesterone, estrogen and prostaglandin F2α treatment. Ten candidate reference genes (ACTR1A, CNOT11, HDAC1, HPRT1, RPL19, RPS9, SDHA, SUZ12, UXT and ZNF131) were evaluated with the use of four approaches (geNorm, NormFinder, BestKeeper, delta Ct). We found that RPS9 and SUZ12 displayed the highest expression stability in the tested material. Moreover, HPRT1 and SDHA were found inappropriate for RT-qPCR data normalization as they demonstrated the highest expression variability out of all candidates analysed. Hence geNorm calculations shown that the use of just two best-performing genes would be sufficient for obtaining reliable results, we propose that RPS9 and SUZ12 be used as suitable endogenous controls in future studies investigating gene expression in normal and compromised pregnancies.

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“…Progesterone concentration in plasma was measured by chemiluminescence (Immunoanalyzer Elecsys and Cobas e, Roche; Jaureguiberry et al, 2020;Madoz et al, 2021). The assay's precision was determined by including control samples with low (1 ng/mL) and high (7 ng/mL) P4 concentrations by duplicates at the beginning and the end of the assay.…”

Section: Determination Of P4 Concentrationsmentioning

confidence: 99%

Detection of nonpregnant cows and potential embryo losses by color Doppler ultrasound and interferon-stimulated gene expression in grazing dairy cows

Madoz

Lorenti²,

Rearte

et al. 2022

Journal of Dairy Science

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Many studies have been conducted to estimate pregnancy losses between 19 and 34 d after artificial insemination (AI) in dairy cows managed under confinementbased systems, but few studies have examined embryo mortality during this interval in dairy cows managed under gazing systems. The objectives of this prospective cohort study were (1) to assess the diagnostic value of the corpus luteum (CL) blood perfusion (BP) evaluation by Doppler ultrasound (US) to detect nonpregnant cows at 19 to 20 d post-AI, and (2) to assess the rate of potential embryo mortality between 19 to 34 d post-AI. The CL-BP of all cows included in the study (n = 131) was examined on farm by power and color mode of Doppler US and later using an image processing software by a second evaluator. The endometrium thickness and echotexture were evaluated by B-mode US at the same visit to assess if the nonpregnancy diagnosis could be improved at 19 to 20 d post-AI by this additional diagnostic tool. Blood samples were obtained at 19 to 20 d post-AI for progesterone (P4) measurement by chemiluminescence and to determine the mRNA expression of ISG by real-time PCR. Pregnancy diagnosis based on embryo visualization was performed at 33 to 34 d post-AI by US B-mode. In parallel interpretation, ISG15 and MX2 mRNA expression in leukocytes [sensitivity (Se), 100%] were regarded as suitable biomarkers for early pregnancy and were selected for molecular characterization of pregnancy at 19 to 20 d post-AI. At 19 to 20 d post-AI, 61.1% of the cows had positive CL-BP by Doppler US (Se, 98.0%), 62.7% had ISG mRNA expression in leukocytes over the cutoff point (Se, 95.7%), and 50.8% were positive, based on the combination of ISG mRNA expression, CL-BP by Doppler US, and P4 concentration (Se, 100%), and were considered as possible pregnant. At 33 to 34 d, the pregnancy rate was 37.4% diagnosed by the B-mode US. Based on the expression of the selected biomarkers in cows with active CL, we found that 28.1% of the cows could have potentially lost their pregnancy between 19 and 34 d post-AI. The Doppler US color mode showed similar accuracy and a higher negative predictive value than the genes selected as biomarkers. The additional B-mode ultrasound evaluation of the uterine stratum vasculare and the endometrium thickness improved the diagnostic accuracy. Therefore, assessing the CL-BP by Doppler US allowed early detection of nonpregnant cows at 19 to 20 d post-AI. The combination of early CL-BP by Doppler US (d 19 to 20) with early embryo detection by B-mode US (d 33-34) could be used to facilitate earlier rebreeding of dairy cows.

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Endometrial expression of key genes related to fertility in repeat breeder and non‐repeat breeder cows

Cited by 7 publications

References 21 publications

CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

CREB1 Is Involved in miR-134-5p-Mediated Endometrial Stromal Cell Proliferation, Apoptosis, and Autophagy

Reference gene selection in bovine caruncular epithelial cells under pregnancy-associated hormones exposure

Detection of nonpregnant cows and potential embryo losses by color Doppler ultrasound and interferon-stimulated gene expression in grazing dairy cows

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