The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered

Pezato, Rogério; Pérez-Novo, Claudina; Holtappels, Gabriële; Ruyck, Natalie De; Crombruggen, Koen Van; Vos, Geert De; Bachert, Claus; Derycke, Lara

doi:10.1016/j.imbio.2014.05.004

Cited by 26 publications

(32 citation statements)

References 36 publications

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“…reported that mDCs and pDCs were elevated in NPs from the Belgium population [28]. This suggests that the elevation of mDCs and pDCs in NPs may be conserved across the American and European populations.…”

Section: Discussionmentioning

confidence: 98%

Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

Poposki

Peterson

Welch

et al. 2015

Clin Experimental Allergy

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Background Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied. Objective The objective of this study was to characterize DC subsets in CRS. Methods We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry. Results Compared to UT from control subjects (n=15) and patients with CRS without NP (CRSsNP) (n=16) and CRSwNP (n=17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n=29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c+ cells but not CD303+ cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a+ cells in NPs might be a subset of mDC1s, and that CD45+CD19-CD1c+CD11c+CD141-CD303-HLA-DR+ mDC1s and CD45+CD19-CD11c+CD1c-CD141high mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45+CD11c-CD303+HLA-DR+ pDCs were only elevated in NPs compared to control UT. Conclusion & Clinical Relevance Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.

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Section: Discussionmentioning

confidence: 98%

Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

Poposki

Peterson

Welch

et al. 2015

Clin Experimental Allergy

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“…[533][534][535] In support of this hypothesis in CRSwNP, polyps exhibit large numbers of type 2 ILCs 536, 537 and dendritic cells. 538,539 The critical upstream epithelial cytokine is not completely clear, but high levels of TSLP activity have been identified in polyp homogenates. 540,541 Some evidence suggests that IL-33 may play a role as well, but these studies have yet to demonstrate increased expression of this cytokine at the protein level in NP tissue.…”

Section: Discussionmentioning

confidence: 99%

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Orlandi

Kingdom

Hwang

et al. 2016

Int Forum Allergy Rhinol

554

888

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Background:The body of knowledge regarding rhinosinusitis (RS) continues to expand, with rapid growth in number of publications, yet substantial variability in the quality of those presentations. In an effort to both consolidate and critically appraise this information, rhinologic experts from around the world have produced the International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICAR:RS). Methods:Evidence-based reviews with recommendations (EBRRs) were developed for scores of topics, using previously reported methodology. Where existing evidence was insufficient for an EBRR, an evidence-based review (EBR) was produced. The sections were then synthesized and the entire manuscript was then reviewed by all authors for consensus. Results:The resulting ICAR:RS document addresses multiple topics in RS, including acute RS (ARS), chronic RS (CRS) with and without nasal polyps (CRSwNP and CRSsNP), recurrent acute RS (RARS), acute exacerbation of CRS (AE-CRS), and pediatric RS. Conclusion:As a critical review of the RS literature, ICAR:RS provides a thorough review of pathophysiology and evidence-based recommendations for medical and surgical treatment. It also demonstrates the significant gaps in our understanding of the pathophysiology and optimal management of RS. Too o en the foundation upon which these recommendations are based is comprised of lowerlevel evidence. It is our hope that this summary of the evidence in RS will point out where additional research efforts may be directed. C 2016 ARS-AAOA, LLC. Key Words:rhinosinusitis; chronic rhinosinusitis; acute rhinosinusitis; recurrent acute rhinosinusitis; evidence-based medicine; systematic review; endoscopic sinus surgery List of Abbreviations Used

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“…These cells are important for the activation of B cell responses and can produce pro-inflammatory cytokines within the tissue. Given the abundance of DC subsets in NP, it is also likely that some T cells can be reactivated and expanded locally [88, 89]. The elevated presence of T cell-attracting chemokines in NP also suggests that new T cells could be continually trafficking to this site to participate in the inflammatory response.…”

Section: Pathogenesis Of Crswnpmentioning

confidence: 99%

Pathogenesis of nasal polyposis

Hulse

Stevens

Tan

et al. 2015

Clin Experimental Allergy

194

191

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Summary Chronic rhinosinusitis with nasal polyps (CRSwNP) is a complex inflammatory condition that affects a large proportion of the population world-wide and is associated with high cost of management and significant morbidity. Yet, there is a lack of population-based epidemiologic studies using current definitions of CRSwNP, and the mechanisms that drive pathogenesis in this disease remain unclear. In this review, we summarize the current evidence for the plethora of factors that likely contribute to CRSwNP pathogenesis. Defects in the innate function of the airway epithelial barrier, including diminished expression of antimicrobial products and loss of barrier integrity, combined with colonization by fungi and bacteria likely play a critical role in the development of chronic inflammation in CRSwNP. This chronic inflammation is characterized by elevated expression of many key inflammatory cytokines and chemokines, including IL-5, thymic stromal lymphopoietin and CCL11, that help to initiate and perpetuate this chronic inflammatory response. Together, these factors likely combine to drive the influx of a variety of immune cells, including eosinophils, mast cells, group 2 innate lymphoid cells and lymphocytes, which participate in the chronic inflammatory response within the nasal polyps. Importantly, however, future studies are needed to demonstrate the necessity and sufficiency of these potential drivers of disease in CRSwNP. In addition to the development of new tools and models to aid mechanistic studies, the field of CRSwNP research also needs the type of robust epidemiologic data that has served the asthma community so well. Given the high prevalence, costs and morbidity, there is a great need for continued research into CRS that could facilitate the development of novel therapeutic strategies to improve treatment for patients who suffer from this disease.

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The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered

Cited by 26 publications

References 36 publications

Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis

International Consensus Statement on Allergy and Rhinology: Rhinosinusitis

Pathogenesis of nasal polyposis

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