The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses

Boon, Jan P.; Zanden, Jelmer J. van; Lewis, Wilma E.; Zegers, Bart N.; Goksøyr, Anders; Arukwe, Augustine

doi:10.1016/s0141-1136(02)00127-7

Cited by 33 publications

(11 citation statements)

References 8 publications

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“…The CYP1A immunopositive protein has been shown to be significantly higher in contaminated environments in response to PAHs and PCBs in the mussel Mytilus sp. 19 , in the sole Pleuronectes vetulus 20 and in the salmon Salmo salar 21 . The results of realtime PCR in this study demonstrated that the CYP1A1 transcript was induced significantly and dose-dependently by BaP-exposure ( Figure 2).…”

Section: Dose-dependent Gene Expression Of Cytochrome P450 1a In Bap mentioning

confidence: 98%

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

Won

Yum

Woo

2011

Toxicol. Environ. Health Sci.

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The construction of a subtractive cDNA library of organisms and the analysis thereof have proven meaningful in the search for clones whose expressions are regulated by exterior changes. The principal objective of this study was to identify the differentially expressed genes of marine medaka fish (Oryzias javanicus) under benzo[a]pyrene exposure conditions. Medaka fish were exposed to 100 μg/L of benzo[a]pyrene for 24 hr and its hepatic RNA was extracted; RNA from the livers of non-exposed medaka fish was also extracted. Both RNAs were employed in the construction of the subtractive cDNA library and the nucleotide sequences of differentially expressed clones were analyzed. Twenty-eight genes of the total differentially expressed clones were considered significant; in particular, the transcription of the cytochrome P450 1A gene was upregulated in a dose-dependent manner under various benzo[a]pyrene concentration exposure conditions. The 28 genes were then divided into 4 categories: (1) information storage and processing, (2) cellular processes and signaling, (3) metabolism, and (4) poorly characterized. The data reported herein provide general information regarding the effects of benzo[a]pyrene contamination on marine organisms, and constitute a primary step in the development of novel biomarkers for marine environmental pollution.

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Section: Dose-dependent Gene Expression Of Cytochrome P450 1a In Bap mentioning

confidence: 98%

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

Won

Yum

Woo

2011

Toxicol. Environ. Health Sci.

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“…Specifically, dose-dependent increases in relative mRNA transcripts encoding vitellogenin (Vtg; egg yolk precursor protein) and zona radiata protein (eggshell protein) were measured in hepatocytes of juvenile male Atlantic salmon (S. solar) exposed to BDE-47 and a PBDE mixture (BDE-47, 153, -154). 226 However, some of these in vitro findings have not been reproduced with in vivo studies of juvenile Atlantic salmon exposed to the PentaBDE mixture 75 or in zebrafish larvae exposed to BDE-47, 95 whereby no change in the expression of Vtg mRNA transcript abundances were detected. Thus, while the in vitro evidence is limited because it may not account for the extensive and coordinated interactions among cells and tissues, conversely the in vivo research conducted to date targeting PBDE effects on male fish reproduction has only been in two species, targeting largely transcriptional changes in immature animals for just a limited number of genes.…”

Section: Pbde Effects On Male Fish Reproductionmentioning

confidence: 98%

“…However, a larger number of PBDE studies in teleost fish have shown no AhR/CYP1A activation. 75,89,97,98 Thus, it appears that PBDEs operate predominantly through non-dioxin, AhR independent toxicity mechanisms and are not metabolized by CYP1A. Conversely, the PentaBDE commercial mixture contains small amounts of polybrominated dibenzo-p-dioxins/dibenzofurans (PBDDs/PBDFs) that are trace byproducts formed by thermal stress during production and that activate the AhR.…”

Section: Mixed Evidence Of Pbde Oxidative Metabolism In Fishmentioning

confidence: 99%

PBDE flame retardants

Noyes

Stapleton

2014

Endocrine Disruptors

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“…It is interesting to note that while in vitro studies all mention the AhRantagonistic effects of BFRs examined, in vivo studies have produced contradictory results. In their studies with Atlantic salmon (Salmo salar) and/or rainbow trout (Oncorhynchus mykkis), Boon et al (2002) and Holm et al (1994) respectively, investigated the effects of exposure to commercial mixtures pentaBDE and octaBDE. The authors found no deviations in the EROD activity.…”

Section: Toxic Effects Of Bfrsmentioning

confidence: 99%

Brominated Flame Retardants in the Environment: their Sources and Effects (A Review)

Mikula

Svobodová

2006

Acta Vet. Brno

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Mikula P., Z. Svobodová: Brominated Flame Retardants in the Environment: Their Sources and Effects (a Review). Acta Vet. Brno 2006, 75: 587-599. Brominated flame retardants (BFRs) are organic substances frequently used in many industries. The most important group within BFRs are polybrominated diphenyl ethers (PBDEs). Because they persist in the environment, accumulate in food chains and have toxic effects, they are a potential health risk both for animals and humans. Polybrominated diphenyl ethers may disrupt processes of hormonal regulation in living organisms by reducing thyroxine concentrations in the plasma of the exposed individuals. In vitro studies have demonstrated the ability of these substances to bind to estrogen and androgen receptors. Tests on rodents have also demonstrated neurotoxicity of some of the PBDEs. Although industrial use of PBDEs is now regulated to a large extent, PBDEs have already been detected in areas with no apparent industrial load, e.g. in Greenland. This article presents an overview of BFRs-related issues with a particular emphasis on PBDEs, describes toxic effects of those substances and their metabolism in living organisms, and discusses issues related to the incidence of PBDEs in the environment. BFRs, polybrominated diphenyl ethers, tetrabromobisphenol A, environmental endocrine disruptors, toxicity, metabolismA large number of chemical substances have recently been under an intensive scrutiny because of their possible adverse health effects on both humans and animals. These substances include the so-called brominated flame retardants (BFRs). They are substances used in a number of industries in the manufacture of polymers and synthetic resins to reduce the risk of fires. Brominated flame retardants are used in building materials (polymer foams, thermal insulations), electric cable insulation, consumer electronics (TV sets, VCRs, computers) and household furnishings (furniture upholstery, carpets, etc.). Brominated flame retardants contain different numbers of bromine atoms which are released from the polymer matrix by heat. They then neutralize energy-rich radicals produced during the combustion process, thus making it more difficult for the process of combustion to continue. Depending on the flame retardant bond in polymer, two groups of BFRs are distinguished. While reactive flame retardants are incorporated into polymer through covalent bonds, additive brominated flame retardants are loosely dispersed in the polymer. Tens or even hundreds of thousands of tonnes of BFRs are produced in the world every year (Table 1). The most frequently used BFRs are tetrabromobisphenol A (TBBPA), commercially produced mixtures of polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD). These substances are released to the environment as a result of extensive industrial exploitation of BFRs. From the point of view of possible negative effects on human and animal health, a lot of attention has been devoted mainly to PBDEs, which may persist in the environment f...

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The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses

Cited by 33 publications

References 8 publications

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

Identification of differentially expressed genes in liver of marine medaka fish exposed to benzo[a]pyrene

PBDE flame retardants

Brominated Flame Retardants in the Environment: their Sources and Effects (A Review)

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