The expression of Bacillus thuringiensis toxin genes in plant cells

Mazier, Marianne; Pannetier, Catherine; Tourneur, J.; Jouanin, Lise; Giband, Marc

doi:10.1016/s1387-2656(08)70039-5

Cited by 30 publications

(9 citation statements)

References 104 publications

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“…The cloning of insecticidal crystal protein genes (Bt) and their expression in transgenic plants provide an alternative strategy to conventional breeding for the protection of crops against insect damage. At least 90 genes encoding protoxins from a wide range of Bacillus thuringiensis isolates have been isolated and sequenced (Mazier et al 1997). In the present study, a large number of rice plants carrying Cry1C gene has been produced in IR64 cultivar by Agrobacterium mediated transformation method.…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Ignacimuthu¹,

Raveendar²

2010

Euphytica

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The rice leaffolder (RLF), Cnaphalocrocis medinalis is an important pest of rice that causes severe damage in many areas of the world. The plants were transformed with fully modified (plant codon optimized) synthetic Cry1C coding sequences as well as with the hpt and gus genes, coding for hygromycin phosphotransferase and b-glucuronidase, respectively. Cry1C sequences placed under the control of doubled 35S promoter plus the AMV leader sequence, and hpt and gus genes driven by cauliflower mosaic virus 35S promoter, were used in this study. Embryogenic calli after cocultivation with Agrobacterium were selected on the medium containing hygromycin B. A total of 67 hygromycinresistant plants were regenerated. PCR and Southern blot analyses of primary transformants revealed the stable integration of Cry1C coding sequences into the rice genome with predominant single copy integration. R 1 progeny plants disclosed a monogenic pattern (3:1) of transgene segregation as confirmed by molecular analyses. These transgenic lines were highly resistant to rice leaffolder (RLF), Cnaphalocrocis medinalis as revealed by insect bioassay.

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Section: Discussionmentioning

confidence: 99%

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Ignacimuthu¹,

Raveendar²

2010

Euphytica

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“…The introduction of Bacillus thuringiensis (Bt) δ-endotoxin genes in crop plants now ranks among the most successful strategies for improving tolerance to certain insects (Mazier et al 1997;Peferoen 1997;Romeis et al 2006). Historically, a limited number of tree species were amenable to genetic transformation, and few transgenic trees with insect resistance could be produced (Jouanin et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Expression of a Bacillus thuringiensis cry1Ab gene in transgenic white spruce and its efficacy against the spruce budworm (Choristoneura fumiferana)

Lachance

Hamel

Pelletier

et al. 2007

Tree Genetics & Genomes

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A synthetic version of the cry1Ab gene from Bacillus thuringiensis (Bt) was introduced into white spruce (Picea glauca) by microprojectile bombardment. A plasmid carrying the cry1Ab gene, driven by a ubiquitin (maize) promoter, was co-transferred with a plasmid containing the gus-nptII fusion gene as a screenable selection marker. Molecular analysis of the transgenic lines showed a high level (more than 90%) of co-integration of the cry1Ab gene with the screenable marker. A wide range of expression levels of the cry1Ab gene and corresponding endotoxin was obtained. Accumulation of the Cry1Ab protein was evaluated in embryogenic tissue, the needles of somatic seedlings and in the needles of 5-year-old field-grown trees of individual lines. Laboratory and field insect feeding trials suggest that several spruce transgenic lines were lethal to spruce budworm larvae.

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“…kurstaki (Roush 1997). A 1997 review article listed 18 species of economic importance that have been successfully transformed with a Bt gene (Mazier et al 1997). This number is continuously increasing, and several transgenic cotton, maize, and potato cultivars have been commercialized since 1995 (Jouanin et al 1998).…”

mentioning

confidence: 99%

Field Evaluation of Soybean Engineered with a Synthetic <I>cry1Ac</I> Transgene for Resistance to Corn Earworm, Soybean Looper, Velvetbean Caterpillar (Lepidoptera: Noctuidae), and Lesser Cornstalk Borer (Lepidoptera: Pyralidae)

Walker¹,

All²,

McPherson³

et al. 2000

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A transgenic line of the soybean 'Jack', Glycine max (L.) Merrill, expressing a synthetic cry1Ac gene from Bacillus thuringiensis variety kurstaki (Jack-Bt), was evaluated for resistance to four lepidopteran pests in the field. Jack-Bt and genotypes serving as susceptible and resistant controls were planted in field cages and artificially infested with larvae of corn earworm, Helicoverpa zea (Boddie), and velvetbean caterpillar, Anticarsia gemmatalis (Hübner), in 1996, 1997, and 1998, and also with soybean looper, Pseudoplusia includens (Walker), in 1996. Susceptible controls included Jack (1996-1998), 'Cobb' (1996), and Jack-HPH (1996). GatIR 81-296 was used as the resistant control in all 3 yr. Compared with untransformed Jack, Jack-Bt showed three to five times less defoliation from corn earworm and eight to nine times less damage from velvetbean caterpillar. Defoliation of GatIR 81-296 was intermediate between that of Jack and Jack-Bt for corn earworm, and similar to that of Jack for velveltbean caterpillar. Jack-Bt exhibited significant, but lower resistance to soybean looper. Jack-Bt also showed four times greater resistance than Jack to natural infestations of lesser cornstalk borer, Elasmopalpus lignosellus (Zeller), in conventional field plots at two locations in 1998. Data from these experiments suggest that expression of this cry1Ac construct in soybean should provide adequate levels of resistance to several lepidopteran pests under field conditions.

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The expression of Bacillus thuringiensis toxin genes in plant cells

Cited by 30 publications

References 104 publications

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Agrobacterium mediated transformation of indica rice (Oryza sativa L.) for insect resistance

Expression of a Bacillus thuringiensis cry1Ab gene in transgenic white spruce and its efficacy against the spruce budworm (Choristoneura fumiferana)

Field Evaluation of Soybean Engineered with a Synthetic <I>cry1Ac</I> Transgene for Resistance to Corn Earworm, Soybean Looper, Velvetbean Caterpillar (Lepidoptera: Noctuidae), and Lesser Cornstalk Borer (Lepidoptera: Pyralidae)

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