The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato

Bucher, Marcel; Brunner, Silvia; Zimmermann, Philip; Zardi, Gerardo I.; Amrhein, Nikolaus; Willmitzer, Lothar; Riesmeier, Jörg W.

doi:10.1104/pp.010998

Cited by 57 publications

(45 citation statements)

References 63 publications

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“…The finding of epidermal marker genes could be important to study cell-specificity associated to epidermal cells in order to have a control for the collected material. RT-PCR experiments with specific primers for a tomato extensin gene (LeEXT1) and an RNaseLX gene (Ko¨ck et al 2006) have shown that LM allows transcripts to be localized in epidermal cells and that, according to Bucher et al (2002), LeEXT1 can be considered an epidermal marker in tomato (RB and JGA, unpublished results).…”

Section: Plant-symbiotic Interactionsmentioning

confidence: 99%

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Balestrini¹,

Gómez-Ariza²,

Klink

et al. 2009

Journal of Plant Interactions

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Laser Microdissection (LM) is a technology that allows the rapid procurement of selected cell populations from a section of heterogeneous tissues in a manner conducive to the extraction of DNA, RNA, proteins and even metabolites. In the past few years, it has also been applied to plant biology in order to study gene expression in plant-nematode and plant-microbe interactions. LM represents a powerful tool since cells associated with a particular infection stage can be visualized under the microscope and harvested. Therefore, verification of the response of the plant during the progression of the colonization can be performed in different cell types. Applications of LM to study the interaction between the plant and both pathogenic and symbiotic organisms (i.e. nematode and fungi, respectively) are explored in this review.

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Section: Plant-symbiotic Interactionsmentioning

confidence: 99%

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Balestrini¹,

Gómez-Ariza²,

Klink

et al. 2009

Journal of Plant Interactions

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“…Judging from their tissue-specific expression, it is argued that extensins and Pro-rich proteins are "tailored to the tissue" during embryogenesis (Zhang et al, 2008) and throughout development (Fowler et al, 1999); this includes root hair formation (Bucher et al, 2002), which also involves a LRX1, a chimera of extensin and Leu-rich repeat protein (Baumberger et al, 2001).…”

Section: What Is the Role Of P3-type Extensin?mentioning

confidence: 99%

Role of the Extensin Superfamily in Primary Cell Wall Architecture

et al. 2011

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“…pLeEXT1:GUS corresponds to the gen1-1:GUS described by Bucher et al (Bucher et al, 2002) and was kindly provided by M. Bucher to A. Niebel (LIPM, Toulouse, France).…”

Section: Plasmid Constructsmentioning

confidence: 99%

“…All NFP amplifications used p2201-NFP (Arrighi et al, 2006) as a template. The gen1-1:GUS construct described by Bucher et al (Bucher et al, 2002) was used as a template for pLeEXT1 and the pGII124-PCO2-HYFT construct (kindly provided by R. Heidstra, Department of Molecular Genetics, Utrecht University, Utrecht, The Netherlands) for pCO2. The different PCR fragments were obtained using the primers specified in supplementary material Table S1.…”

Section: Plasmid Constructsmentioning

confidence: 99%

Epidermal and cortical roles ofNFPandDMI3in coordinating early steps of nodulation inMedicago truncatula

et al. 2012

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SUMMARYLegumes have evolved the capacity to form a root nodule symbiosis with soil bacteria called rhizobia. The establishment of this symbiosis involves specific developmental events occurring both in the root epidermis (notably bacterial entry) and at a distance in the underlying root cortical cells (notably cell divisions leading to nodule organogenesis). The processes of bacterial entry and nodule organogenesis are tightly linked and both depend on rhizobial production of lipo-chitooligosaccharide molecules called Nod factors. However, how these events are coordinated remains poorly understood. Here, we have addressed the roles of two key symbiotic genes of Medicago truncatula, the lysin motif (LysM) domain-receptor like kinase gene NFP and the calcium-and calmodulindependent protein kinase gene DMI3, in the control of both nodule organogenesis and bacterial entry. By complementing mutant plants with corresponding genes expressed either in the epidermis or in the cortex, we have shown that epidermal DMI3, but not NFP, is sufficient for infection thread formation in root hairs. Epidermal NFP is sufficient to induce cortical cell divisions leading to nodule primordia formation, whereas DMI3 is required in both cell layers for these processes. Our results therefore suggest that a signal, produced in the epidermis under the control of NFP and DMI3, is responsible for activating DMI3 in the cortex to trigger nodule organogenesis. We integrate these data to propose a new model for epidermal/cortical crosstalk during early steps of nodulation.

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The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato

Cited by 57 publications

References 63 publications

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Application of Laser Microdissection to plant pathogenic and symbiotic interactions

Role of the Extensin Superfamily in Primary Cell Wall Architecture

Epidermal and cortical roles ofNFPandDMI3in coordinating early steps of nodulation inMedicago truncatula

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