The expression levels of the translational factors eEF1A 1/2 correlate with cell growth but not apoptosis in hepatocellular carcinoma cell lines with different differentiation grade

Grassi, Gabriele; Scaggiante, Bruna; Farra, Rossella; Dapas, Barbara; Agostini, Francesco; Daniele, Bruno; Rosso, Natalia; Tiribelli, Claudio

doi:10.1016/j.biochi.2007.07.007

Cited by 65 publications

(59 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides translation initiation factors, peptide elongations factors have also been implicated in carcinogenesis (10,40). Beyond its housekeeping role in protein synthesis, the peptide elongation factor eEF1A2 acts as an oncogene for cancers of the breast (12), liver (13), lung (14), pancreas (41), and ovary (10).…”

Section: Discussionmentioning

confidence: 99%

“…According to this study, the overexpression of eEF1A2 causes transformation of rodent fibroblasts (NIH3T3) cells and stimulates the growth of ovarian cancer (ES-2) cell xenograft in nude mice (10). Subsequent studies have revealed that eEF1A2 is overexpressed in primary breast tumors (12) and is positively correlated with the growth of hepatocellular carcinoma cells (13). Recently, eEF1A2 has been recognized as a biomarker for cancers of breast (12), lung (14), and prostate (15).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Resveratrol Suppresses Growth of Human Ovarian Cancer Cells in Culture and in a Murine Xenograft Model: Eukaryotic Elongation Factor 1A2 as a Potential Target

Lee¹,

Choi²,

Kundu³

et al. 2009

Cancer Research

View full text Add to dashboard Cite

The eukaryotic elongation factor 1A2 (eEF1A2) is known to retain oncogenic potential and is recognized as a novel target for cancer prevention and therapy. Resveratrol (trans-3,4 ¶,5-trihydroxystilbene), a phytoalexin present in grapes, has been reported to possess chemopreventive and chemotherapeutic activities. In the present study, we examined the growth-inhibitory effects of resveratrol in human ovarian cancer PA-1 cells, considering eEF1A2 as a potential molecular target. Pretreatment with resveratrol attenuated proliferation of serum-starved PA-1 cells stimulated with insulin or serum. Resveratrol also activated caspase-9, -7, and -3 and induced apoptosis in PA-1 cells in the presence of insulin or serum. Insulin or serum stimulation of PA-1 cells resulted in the marked induction of eEF1A2, which was suppressed by pretreatment with resveratrol. Moreover, resveratrol inhibited insulin-or serum-induced soft-agar colony formation in eEF1A2-transfected NIH3T3 cells. An antibody array directed to assess the phosphorylation of protein kinases revealed that treatment with insulin or serum induced the phosphorylation of Akt in PA-1 cells. Pharmacologic inhibition of Akt with LY294002 abrogated insulin-or serum-induced eEF1A2 expression and increased the caspase-3 activity. In another experiment, i.p. administration of resveratrol retarded the growth of PA-1 cell xenograft and the expression of eEF1A2 in athymic nude mice in association with decreased bromodeoxyuridine positivity, reduced expression of proliferating cell nuclear antigen, increased the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and caspase-3 staining, and diminished CD31 positivity. Taken together, eEF1A2 may be considered as a potential molecular target for the antiproliferative effects of resveratrol in PA-1 ovarian cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Resveratrol Suppresses Growth of Human Ovarian Cancer Cells in Culture and in a Murine Xenograft Model: Eukaryotic Elongation Factor 1A2 as a Potential Target

Lee¹,

Choi²,

Kundu³

et al. 2009

Cancer Research

View full text Add to dashboard Cite

show abstract

“…Although eEF1A2 maps on chromosome 20q13, a region suspected to harbor oncogenes and related to gene amplification in many tumors, Anand et al and Grassi et al reported that overexpression of eEF1A2 did not completely depend on the genomic status of this locus (Anand et al 2002;Grassi et al 2007), suggesting the existence of alternative mechanisms. Furthermore, phosphorylation and expression of eEF1A2 were reported to be modulated by Raf, an important oncogene involved in the Ras/Raf/ERK1/2 signaling pathway (Lamberti et al 2007;Olson and Hallahan 2004;Sanges et al 2012).…”

Section: Fig 3 Continuedmentioning

confidence: 96%

Overexpression of eukaryotic elongation factor 1 alpha-2 is associated with poorer prognosis in patients with gastric cancer

Yang

Chen

et al. 2015

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

show abstract

“…The activated type I receptor phosphorylates Smad2 and eEF1A is also involved in the tumorigenesis. The increased expression of eEF1A1 was observed in hepatocellular carcinoma, 7,8 and prostate carcinoma. eEF1A increased metastatic potential of mammary adenocarcinoma.…”

Section: Tgfβ Signalingmentioning

confidence: 97%

Translational connection of TGFβ signaling

Lin

Souchelnytskyi²

2011

Small GTPases

View full text Add to dashboard Cite

The expression levels of the translational factors eEF1A 1/2 correlate with cell growth but not apoptosis in hepatocellular carcinoma cell lines with different differentiation grade

Cited by 65 publications

References 32 publications

Resveratrol Suppresses Growth of Human Ovarian Cancer Cells in Culture and in a Murine Xenograft Model: Eukaryotic Elongation Factor 1A2 as a Potential Target

Resveratrol Suppresses Growth of Human Ovarian Cancer Cells in Culture and in a Murine Xenograft Model: Eukaryotic Elongation Factor 1A2 as a Potential Target

Overexpression of eukaryotic elongation factor 1 alpha-2 is associated with poorer prognosis in patients with gastric cancer

Translational connection of TGFβ signaling

Contact Info

Product

Resources

About