Overexpression of eukaryotic elongation factor 1 alpha-2 is associated with poorer prognosis in patients with gastric cancer

Yang, Song; Lu, Mingdian; Chen, Yuanyuan; Meng, Delong; Sun, Ruochuan; Yun, Dapeng; Zhao, Z.; Lu, Daru; Li, Yongxiang

doi:10.1007/s00432-014-1897-7

Cited by 23 publications

(27 citation statements)

References 41 publications

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“…Its high expression has been found in many cancer tissues of the breast, ovary, and lung (24)(25)(26). eEF1A2 was upregulated in gastric cancer and was an independent indicator to predict poor prognosis in patients with gastric cancer (27), but the expression of eEF1A1 in normal and cancerous gastric tissues has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

P21 activated kinase 4 binds translation elongation factor eEF1A1 to promote gastric cancer cell migration and invasion

2017

Oncology Reports

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P21 activated kinase 4 (PAK4), as an effector of Cdc42, playing important roles in regulating the processes of cytoskeleton organization. PAK4 has been considered to be an oncogenic protein, which has strong relationship with gastric cancer metastasis. However, the mechanism of PAK4 in regulating gastric cancer metastasis is still not fully understood. In this study, using yeast two-hybrid system, we identified that the eukaryotic elongation factor 1 α1 (eEF1A1) is a new binding partner of PAK4. The interaction between PAK4 and eEF1A1 was confirmed by GST pull-down and co-immunoprecipitation. PAK4 co-localized with eEF1A1 in the cytoplasm of gastric cancer cells. Overexpression of PAK4 enhanced the expression level of eEF1A1 and vice versa. PAK4 and eEF1A1 could cooperate to promote gastric cancer cell migration and invasion. Furthermore, the expression of PAK4 and eEF1A1 in clinical gastric cancer samples were examined by western blotting and immunohistochemistry. Statistical analysis indicated that there was positive correlation between the expression of PAK4 and eEF1A1. This study demonstrated for the first time that PAK4 interacted with eEF1A1 to promote migration and invasion of gastric cancer cells, thereby providing new insights into the function of PAK4 and eEF1A1 in the progression of gastric cancer.

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Section: Introductionmentioning

confidence: 99%

P21 activated kinase 4 binds translation elongation factor eEF1A1 to promote gastric cancer cell migration and invasion

2017

Oncology Reports

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“…Duanmin et al have shown that the increased expression of eEF1A2 in pancreatic ductal adenocarcinoma was closely associated with nodal metastasis, perineural invasion and worse prognosis [17]. In the study of Yang et al, the over-expression of eEF1A2 was con rmed to be correlated with worse outcomes in gastric cancer patients [29]. Moreover, Kawamura et al reported that the positive expression of eEF1A2 in non-small cell lung cancer indicated poor prognosis [30].…”

Section: Discussionmentioning

confidence: 99%

EEF1A Acts as A Promising Novel Bio-Marker in the Prognosis of Patients With Cholangiocarcinoma

Wang¹,

Y²,

Ying³

et al. 2020

Preprint

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Background: The morbidity and mortality of cholangiocarcinoma (CCA) is increasing in recent years. EEF1A2 could regulate multiple biological and pathological processes. Our study was designed to investigate the clinical significance of eEF1A2 for prognosis evaluation in CCA.Methods: The quantitative analysis for eEF1A2 in CCA specimens was performed using quantitative real-time polymerase chain reaction (qRT-PCR). The influences of eEF1A2 expression on disease progression and survival of CCA patients were analyzed by Chi-square test and Kaplan-Meier method, respectively. Additionally, cox regression analyses were adopted to identify the potential predictive biomarker for prognosis of CCA.Results: eEF1A2 expression showed increased tendency in CCA tissues (P<0.05). The expression profile of eEF1A2 was positively correlated with TNM stage (P=0.004), lymph node metastasis (P=0.001) and distant metastasis (P=0.001). In addition, high eEF1A2 expression predicted dismal survival among CCA cases (P<0.05). EEF1A2 might be independently correlated with prognosis of CCA (HR=2.724, 95%CI=1.303-5.964, P=0.008).Conclusions: eEF1A2 may act as a predictive biomarker for clinical outcomes of CCA.

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“…IHC and scoring. The immunohistochemical assay was performed as previously described (19,20). Briefly, H&E-stained slides were screened to identify optimal intratumoral tissue to construct a TMA (Shanghai Biochip Company, Ltd.) before IHC.…”

Section: Rna Preparation and Reverse Transcription-quantitative Polymmentioning

confidence: 99%

Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma

Meng

You

et al. 2020

Oncol Rep

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Multiple regions in the short arm of chromosome 3 are frequently deleted in a variety of solid tumors including gallbladder carcinoma (GBC). RNA binding motif, single-stranded interacting protein 3 (RBMS3), a tumor suppressor gene (TSG), is located in this region. However, the role of RBMS3 in GBC remains unclear. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting were performed to evaluate the mRNA and protein expression levels of RBMS3 in 41 fresh frozen GBC tissues and paired normal tissues. An immunohistochemical assay was performed on a tissue microarray (TMA, consisting of 125 cases GBC and 47 normal controls). Microvessel density (MVD) counts were determined using CD34 immunohistochemical staining. Moreover, univariate and multivariate analyses were performed to determine the correlations between RBMS3 expression, MVD and patient prognosis. Cellular functions including proliferation, clonogenicity and apoptosis, were assessed to further identify in vitro roles of RBMS3. It was revealed that both mRNA and protein expression levels of RBMS3 were significantly lower in GBC tissues than in normal controls. Multivariate Cox regression analyses demonstrated cytoplasmic RBMS3 expression as an independent prognostic factor correlated with GBC angiogenesis, histo-pathological differentiation and TNM stage. Kaplan-Meier curves revealed that patients with lower cytoplasmic RBMS3 levels had a significantly worse OS than patients with higher cytoplasmic RBMS3 expression. Additionally, ectopic expression of RBMS3 markedly suppressed GBC cell proliferation and clonogenicity and promoted apoptosis in vitro. These findings indicated the potential of cytoplasmic RBMS3 as a tumor prognostic biomarker and a promising therapeutic target for GBC.

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Overexpression of eukaryotic elongation factor 1 alpha-2 is associated with poorer prognosis in patients with gastric cancer

Abstract: Our results showed for the first time that overexpression of eEF1A2 was correlated with worse outcomes in gastric cancer patients, suggesting its critical roles in the carcinogenesis of gastric cancer.

Cited by 23 publications

References 41 publications

P21 activated kinase 4 binds translation elongation factor eEF1A1 to promote gastric cancer cell migration and invasion

P21 activated kinase 4 binds translation elongation factor eEF1A1 to promote gastric cancer cell migration and invasion

EEF1A Acts as A Promising Novel Bio-Marker in the Prognosis of Patients With Cholangiocarcinoma

Increased expression of RBMS3 predicts a favorable prognosis in human gallbladder carcinoma

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