The expression and role of β3AR protein in myocardial ischemia/reperfusion in rats

Abstract

IntroductionTo explore serum norepinephrine (NE) concentration and β3-adrenoceptor (β3AR) protein expression at different times during myocardial ischemia/reperfusion (I/R) and examine the role of β3AR in I/R.Material and methods28 Sprague-Dawley (SD) rats were randomly divided into one sham group and six I/R groups. The rats in the I/R groups were subjected to ischemia for 45 minutes. After reperfusion, the serum NE concentration and the β3AR protein expression in the myocardial tissue of the left ventricular injury region were detected. Another 18 SD rats were randomly divided into a sham group, I/R groups, and I/R + BRL37344 group.ResultsCompared with the sham group, the serum NE concentration of rats in the I/R groups significantly increased at 6 hours (P < 0.001). The serum NE concentration and myocardial β3AR protein expression were both highest at 72 hours. Compared with the sham group, the expressions of the pro-apoptotic proteins Bax and cleaved caspase-3 after I/R were significantly increased (P < 0.01, P < 0.001, respectively), and the expression of anti-apoptotic protein Bcl-2 was significantly decreased (P < 0.01). Compared with I/R groups, the expressions of Bax and cleaved caspase-3 in the I/R + BRL37344 group were significantly decreased (P < 0.05, P < 0.01, respectively).ConclusionsWith the prolongation of myocardial I/R in rats, serum NE concentration and β3AR protein expression showed a significant increase trend and reached a peak at 72 hours. Specific β3AR agonist BRL37344 can reduce myocardial I/R injury in vivo in rats, alleviate apoptosis, reduce infarct size, and improve cardiac function.

Pre-treatment of the beta3-adrenergic receptor agonist BRL37344 reduces in vivo myocardial ischemia/reperfusion injury by improving AMPK and SIRT1 activity and by suppressing mTOR and p70S6K signaling pathways

“…Left ventricular function was significantly improved in the BRL-treated groups (García-Prieto et al, 2014). PostT of BRL in I/R reduced apoptosis, reduced infarct size and improved cardiac function (Wang et al, 2021). β3-ARs activation has an important effect on the improvement of heart function (Niu et al, 2012).…”

Assessment of Apoptosis-Related Genes in Autografted/ Vitrified Ovarian Tissue in Mice

“…41 Ischemia injury is associated with apoptosis incidence in several organs and survival rate. 29,42 In current study, we hypothesized that ischemia of the ovarian tissues can alter expression of p53 and Bcl-2. In this regard we evaluate the effect of different concentrations of cryoprotectant solutions on the expression of Bax, Bcl-2, P53 and Bid expression on fresh and cryopreserved-thawed ovarian tissue.…”