The Expression and Relationship of CD68-Tumor-Associated Macrophages and Microvascular Density With the Prognosis of Patients With Laryngeal Squamous Cell Carcinoma

Abstract

Objectives.We sought to identify the expression of CD68-tumor-associated macrophages (TAMs) and CD34-microvascular density (MVD) in laryngeal squamous cell carcinoma (LSCC), to study the relationship with clinical pathological parameters and to determine whether their expression is predictive of disease.Methods.Pathologically confirmed 45 LSCC tissue and 20 peritumoral non-tumor tissue were examined. Immunohistochemical studies were used to detect the expression of CD68-TAMs and CD34-MVD.Results.The positive expression rate of CD68 in LSCC tissue was 82% (37/45), which was higher than the 10% (2/20) expression rate of the peritumoral tissue (P<0.05). The CD34-MVD positive expression rate in the LSCC tissue was 26.5±6.4, which obviously higher than 12.2±4.0 expression rate of the peritumoral tissue (P<0.05). The positive expression rates of both CD68 and CD34-MVD were higher in the lymph node metastasis (LNM) positive group than in the LNM negative group. The expression of CD68 had positive correlation with CD34-MVD. The 5-year disease-free survival rate in the group with the low CD68 expression was significantly higher than that in the group with high CD68 expression (76% vs. 42%, respectively).Conclusion.The high expression of CD68-TAMs in LSCC and its positive correlation with CD34-MVD illustrates that both play an important role in promoting the metastasis and angiogenesis of this cancer. Their expression was also positively correlated with the prognoses of these patients, suggesting that they could be used as important prognostic markers for LSCC.

Diminished CD68+ Cancer-Associated Fibroblast Subset Induces Regulatory T-Cell (Treg) Infiltration and Predicts Poor Prognosis of Oral Squamous Cell Carcinoma Patients

“…For example, the higher the CD68 þ TAM infiltrating density in laryngeal squamous cell carcinoma, the worse the prognosis. 35 Also, increased numbers of CD68 þ TAMs indicate an adverse overall outcome in Hodgkin lymphoma. 36 However, the prognostic value of CD68 þ CAF infiltration in tumor has never been revealed, and only a few studies found the CD68 expression in fibroblasts without further research.…”

Differential association of CD68+ and CD163+ macrophages with macrophage enzymes, whole tumour gene expression and overall survival in advanced melanoma

“…Previous reports have similarly reported that the presence of CD68 + cells could be a negative or a positive prognostic marker across tumours of the same subtype. 23 …”

Microvascular density and hypoxia-inducible factor in intraepithelial vocal fold lesions

“…They suggested that MVD CD34 has an important role in promoting metastases in laryngeal cancer and can serve as a prognostic marker [17]. Popov et al reported a mean MVD with CD34 in a study group of laryngeal cancer of 14.27 ± 12.92 and confirmed a significantly higher MVD in patients with metastasis than in those without [18].…”

“…Sun et al compared MVD CD34 in laryngeal cancer (26.5 ± 6.4) with the peritumoural tissue (12.2 ± 4.0) and found a significant difference ( p < 0.05) among them, but also confirmed a higher MVD CD34 in patients with lymph node metastasis than that in the N0 group [ 17 ]. They suggested that MVD CD34 has an important role in promoting metastases in laryngeal cancer and can serve as a prognostic marker [ 17 ]. Popov et al reported a mean MVD with CD34 in a study group of laryngeal cancer of 14.27 ± 12.92 and confirmed a significantly higher MVD in patients with metastasis than in those without [ 18 ].…”