The expression and proangiogenic effect of nucleolin during the recovery of heat-denatured HUVECs

Liang, Pengfei; Jiang, Bimei; Lv, Chunliu; Huang, Xu; Sun, Li; Zhang, Pihong; Huang, Xiaoyuan

doi:10.1016/j.bbagen.2013.05.027

Cited by 26 publications

(39 citation statements)

References 25 publications

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“…The heat‐denatured cell model was established according to our previous studies . When their growth reached 70–80% confluence, the cells were digested with trypsin (5 mg/mL)(Gibco), and prepared with the cell density of 2 × 10 5 cells/mL with DMEM medium.…”

Section: Methodsmentioning

confidence: 99%

“…It is involved in a wide range of essential cell functions including DNA and RNA metabolism, chromatin structure, rDNA transcription, rRNA maturation, cytokinesis, nucleogenesis, cell proliferation, and growth . Recently, we have found that nucleolin is up‐regulated, and plays a proangiogenic role during the recovery of heat‐denatured dermis likely due to the production of vascular endothelial growth factor . These data raise the possibility that nucleolin could be an endogenous protective protein in endothelial cells and regulate the functional recovery of the heat‐denatured dermis.…”

Section: Introductionmentioning

confidence: 94%

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Nucleolin enhances the proliferation and migration of heat‐denatured human dermal fibroblasts

Jiang

Liang

et al. 2015

Wound Repair Regeneration

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Denatured dermis, a part of dermis in burned skin, has the ability to restore its normal morphology and functions after their surrounding microenvironment is improved. However, the cellular and molecular mechanisms by which the denatured dermis could improve wound healing are still unclear. This study aimed to investigate the role of nucleolin during the recovery of heat-denatured human dermal fibroblasts. Nucleolin mRNA and protein expression were significantly increased time-dependently during the recovery of heat-denatured human dermal fibroblasts (52 °C, 30 seconds). Heat-denaturation promoted a time-dependent cell proliferation, migration, chemotaxis, and scratched wound healing during the recovery of human dermal fibroblasts. These effects were prevented by knockdown of nucleolin expression with small interference RNA (siRNA), whereas overexpression of nucleolin enhanced cell proliferation, migration, and chemotaxis of human dermal fibroblasts with heat-denaturation. In addition, the expression of transforming growth factor-beta 1(TGF-β1) was significantly increased during the recovery of heat-denatured dermis and human dermal fibroblasts. TGF-β1 expression was up-regulated by nucleolin in human dermal fibroblasts. The results suggest that nucleolin expression is up-regulated, and play an important role in promoting cell proliferation, migration, and chemotaxis of human dermal fibroblasts during the recovery of heat-denatured dermis with a mechanism probably related to TGF-β1.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Nucleolin enhances the proliferation and migration of heat‐denatured human dermal fibroblasts

Jiang

Liang

et al. 2015

Wound Repair Regeneration

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“…It was shown that both the MVD (CD31+ endothelial cells) and VEGF‐A expressions (mRNA and protein) of ischaemic myocardium in HSYA‐treated AMI mice were substantially elevated than those of saline‐treated AMI mice ( P < .05, Figure 3A–D). In our previous study, nucleolin was proved to play a pro‐angiogenic role during the recovery of heat‐denatured dermis through enhancing the production of VEGF‐A 20. So in this study, the expression of nucleolin was also detected, and we found that both the mRNA and protein expressions of nucleolin were significantly increased in the myocardium of AMI mice compared with those of the sham‐operated controls, whereas HSYA further enhanced the mRNA and protein expressions of nucleolin in the myocardium of AMI mice ( P < .05, Figure 3C,D).…”

Section: Resultsmentioning

confidence: 99%

“…As we reported previously,20 the migration of HUVEC was determined by the scratch wound‐healing assay. Six‐well plates were seeded with cells to a final density of 2 × 10 5 cells per well, and a 10‐μL sterile pipette tip was used to scratch at the mid of each well until the cells were adherent to the plate.…”

Section: Methodsmentioning

confidence: 97%

“…Nucleolin regulates the mRNA stability of target genes and consequently mediates their post‐transcriptional control through binding the nucleolin recognition element to corresponding binding elements of different target genes 17, 18, 19. It has been reported that nucleolin can mediate the expressions of angiogenesis‐related genes, including vascular endothelial growth factor‐A (VEGF‐A) and matrix metalloproteinase‐9 (MMP‐9) 14, 20, 21. However, whether nucleolin mediates the pro‐angiogenic effect of HSYA through post‐transcriptional regulation of these angiogenesis‐related genes is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Nucleolin mediated pro‐angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post‐transcriptional regulation of VEGF‐A and MMP‐9

Zou

Wang

Liu

et al. 2018

J Cellular Molecular Medi

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Hydroxysafflor Yellow A (HSYA), a most representative ingredient of Carthamus tinctorius L., had long been used in treating ischaemic cardiovascular diseases in China and exhibited prominently anticoagulant and pro‐angiogenic activities, but the underlying mechanisms remained largely unknown. This study aimed to further elucidate the pro‐angiogenic effect and mechanism of HSYA on ischaemic cardiac dysfunction. A C57 mouse model of acute myocardial infarction (AMI) was firstly established, and 25 mg/kg HSYA was intraperitoneally injected immediately after operation and given once, respectively, each morning and evening for 2 weeks. It was found that HSYA significantly improved ischaemia‐induced cardiac haemodynamics, enhanced the survival rate, alleviated the myocardial injury and increased the expressions of CD31, vascular endothelial growth factor‐A (VEGF‐A) and nucleolin in the ischaemic myocardium. In addition, HSYA promoted the migration and tube formation of human umbilical vein endothelial cells (HUVECs), enhanced the expressions of nucleolin, VEGF‐A and matrix metalloproteinase‐9 (MMP‐9) in a dose‐ and time‐dependent manner. However, down‐regulation of nucleolin expression sharply abrogated the effect mentioned above of HSYA. Further protein‐RNA coimmunoprecipitation and immunoprecipitation‐RT‐PCR assay showed that nucleolin binded to VEGF‐A and MMP‐9 mRNA and overexpression of nucleolin up‐regulated the mRNA expressions of VEGF‐A and MMP‐9 in the HUVECs through enhancing the stability of VEGF‐A and MMP‐9 mRNA. Furthermore, HSYA increased the mRNA expressions of VEGF‐A and MMP‐9 in the extract of antinucleolin antibody‐precipitated protein from the heart of AMI mice. Our data revealed that nucleolin mediated the pro‐angiogenic effect of HSYA through post‐transcriptional regulation of VEGF‐A and MMP‐9 expression, which contributed to the protective effect of HSYA on ischaemic cardiac dysfunction.

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Nucleolin protects against doxorubicin‐induced cardiotoxicity via upregulating microRNA‐21

Sun

Tong

Fang

et al. 2018

Journal Cellular Physiology

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Nucleolin is a multifunctional protein and participates in many important biological processes. Our previous study found that nucleolin protects the heart against myocardial ischemia-reperfusion injury. In this study, we aimed to investigate the role of nucleolin in doxorubicin (DOX)-induced cardiotoxicity. The expression pattern of nucleolin in hearts subjected to DOX injury was investigated, and we found that administration of DOX induced nucleolin expression significantly in vivo and in vitro. Gene transfection and RNA interference approaches were used in cardiomyocytes to investigate the function of nucleolin. Nucleolin overexpression protects cardiomyocytes against DOX-induced injury. Nucleolin-ablated cardiomyocytes become susceptible to the injury induced by DOX. The hearts of cardiac-myocyte-specific nucleolin transgenic mice are more resistant to DOX injury. Furthermore, nucleolin upregulates microRNA(miRNA)-21 expression in vivo and in vitro, and the miRNA-21 inhibitor negates the protective effect of nucleolin against injury induced by DOX. These results have demonstrated that nucleolin is involved in the regulation of DOX-induced cardiac injury and dysfunction via the regulation of miRNA-21 expression, and may be a novel therapeutic target for DOX-induced cardiotoxicity.

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The expression and proangiogenic effect of nucleolin during the recovery of heat-denatured HUVECs

Cited by 26 publications

References 25 publications

Nucleolin enhances the proliferation and migration of heat‐denatured human dermal fibroblasts

Nucleolin enhances the proliferation and migration of heat‐denatured human dermal fibroblasts

Nucleolin mediated pro‐angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post‐transcriptional regulation of VEGF‐A and MMP‐9

Nucleolin protects against doxorubicin‐induced cardiotoxicity via upregulating microRNA‐21

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