The exposure of healthy volunteers to 200 ppm 1,1,1-trichloroethane increases the concentration of proinflammatory cytokines in nasal secretions

Muttray, Axel; Klimek, Ludger; Faas, Michael; Schäfer, Dirk; Mann, Wolf J.; Konietzko, J

doi:10.1007/s004200050403

Cited by 21 publications

(20 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Short-term exposure to ozone has been shown to result in elevated PGE 2 concentrations in human bronchoalveolar fluid [27]. In two recent studies, we did not find any significant changes in the concentration of PGE 2 after 4 h of exposure to 200 ppm of methanol [28] or to 200 ppm of trichlorethane in vivo [24], 200 ppm being the occupational threshold limit for both of these substances in Germany. This indicated that PGE 2 was not sensitive enough as a marker to detect subclinical inflammatory irritations after exposure to low concentrations of these substances at the level of the occupational threshold limit in vivo.…”

Section: Discussionmentioning

confidence: 69%

Evaluation of Inflammatory Reactions and Genotoxic Effects after Exposure of Nasal Respiratory Epithelia to Benzene

Gosepath¹,

Grebneva²,

Brieger³

et al. 2003

ORL

View full text Add to dashboard Cite

Background: The aim of this study was to identify inflammatory changes as well as genotoxic effects in cultivated human respiratory epithelial cells after in vitro exposure to benzene. Methods: Primary cell cultures of nasal respiratory mucosa were exposed to synthetic air enriched with 5,000 µg/m³ of benzene at an air/liquid interface over 8 h and then to synthetic air only over the following 24 h. Controls were continuously exposed to synthetic air over 32 h. To detect inflammatory reactions, release of prostaglandin E₂ was quantified using a competitive enzyme immunoassay. The Comet Assay was used to quantify the ratio of apoptotic cells with benzene-induced DNA fragmentation. Results: Prostaglandin release as well as DNA fragmentation increased after 8 h of exposure and remained elevated throughout the following 24 h but did not increase in controls. Conclusions: High concentrations of benzene induce an inflammatory response and possibly fragmentation of DNA in respiratory epithelial cells. These findings have to be discussed with respect to possible mutagenic or carcinogenic effects of benzene in nasal respiratory cells.

show abstract

Section: Discussionmentioning

confidence: 69%

Evaluation of Inflammatory Reactions and Genotoxic Effects after Exposure of Nasal Respiratory Epithelia to Benzene

Gosepath¹,

Grebneva²,

Brieger³

et al. 2003

ORL

View full text Add to dashboard Cite

show abstract

“…in response to inoculation with bacterial or viral pathogens [1][2][3], allergen challenge [4][5][6], or exposure to environmental pollutants [7][8][9], have focused on the detection of minute amounts of cytokines and inflammatory mediators. For this purpose, various methods to collect nasal secretions are employed (table 1), yielding heterogeneous matrices and analyte concentrations.…”

mentioning

confidence: 99%

Biological markers in nasal secretions

Riechelmann¹,

Deutschle²,

Friemel³

et al. 2003

Eur Respir J

View full text Add to dashboard Cite

Biological markers in nasal secretions provide valuable information on nasal pathophysiology. However, published data on biomarker concentrations in nasal fluids are remarkably inconsistent, and the bias due to different sampling techniques, has not yet been systematically evaluated.Concentrations of various protein were repeatedly determined in nasal secretions of 16 healthy volunteers. The proteins were detected by using: 1) a 2 -macroglobulin as a marker for plasma contamination; 2) lactoferrin as a marker for glandular secretion; 3) lactate dehydrogenase as a marker for tissue injury; and 4) interleukin (IL)-1b, IL-8, tumour necrosis factor-a, and eosinophil cationic protein and tryptase as indicators for tissue inflammation. A total of four different sampling methods, including nasal lavage (NL) and a new polyurethane foam sampler technique (PFST) were employed.Analyte concentrations in NL were approximately10-times lower than in specimens obtained by PFST. Due to the unpredictable dilution during NL, various analytes were below the detection limit of the high sensitivity assays employed. With PFST, concentrations below the detection limit rarely occurred. The specimens did not significantly differ regarding plasma contamination, glandular secretion or tissue injury.The considerable variability of reported analyte concentrations in nasal secretions mainly results from different sampling techniques. To collect nasal secretions, samplers are considered superior to nasal lavage techniques. Eur Respir J 2003; 21: 600-605.

show abstract

“…200 ppm 1,1,1-trichloroethane and methanol induced subclinical inflammation of the nasal mucosa, whereas the functional parameters of mucociliary transport time and ciliary beat frequency of the respiratory cells remained unchanged [13,17]. The concentrations corresponded to the MAK values.…”

Section: Introductionmentioning

confidence: 66%

Effects of an external exposure to 200 ppm methyl ethyl ketone on nasal mucosa in healthy volunteers

Muttray

Jung

Klimek³

et al. 2002

IAOEH

Self Cite

View full text Add to dashboard Cite

show abstract

The exposure of healthy volunteers to 200 ppm 1,1,1-trichloroethane increases the concentration of proinflammatory cytokines in nasal secretions

Abstract: The interleukins measured proved to be sensitive indicators of irritating effects of 1,1, 1-trichloroethane. The German threshold limit (MAK value) of 200 ppm 1,1,1-trichloroethane does not prevent the subclinical inflammation of nasal mucosa.

Cited by 21 publications

References 16 publications

Evaluation of Inflammatory Reactions and Genotoxic Effects after Exposure of Nasal Respiratory Epithelia to Benzene

Evaluation of Inflammatory Reactions and Genotoxic Effects after Exposure of Nasal Respiratory Epithelia to Benzene

Biological markers in nasal secretions

Effects of an external exposure to 200 ppm methyl ethyl ketone on nasal mucosa in healthy volunteers

Contact Info

Product

Resources

About