The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents

Staphylococcus saprophyticus Proteomic Analyses Elucidate Differences in the Protein Repertories among Clinical Strains Related to Virulence and Persistence

“…For example, the highly capsular 9325 strain secretes higher content of antigenic proteins and transglycosylases while the non-capsular 7108 strain does not secrete the SsaA antigenic protein and secretes higher content of proteases. These results show diversity in protein secretion among strains [15].…”

“…S. saprophyticus can compose the skin microbiota and act as opportunist bacteria. Studies with contact sports athletes demonstrates high S. saprophyticus prevalence in the skin, suggesting contact Pathogens 2020, 9,69 3 of 14 during 18 hours also shows these strains possess similar cell growth curves [15]. We speculate that the capsule size can interfere in the optical density and can explain the differences shown in Figure S1.…”

“…In counterpart, the 9325 strain does not accumulate urease inside the cell and the secretion system is more efficient, since the urease activity inside the cell is low and the urease activity outside the cell is very high, being detected in all the cell dilutions tested. The S. saprophyticus strains used in this work were previously analyzed and also differ in the exoproteome content [15]. In order to compare if the phenotypic, exoproteomic, and proteomic differences described for these strains could reflect the ability to survive during interaction with host cells, we performed interaction assays of S. saprophyticus cells with macrophages.…”

“…The scheme is shown in Figure 4. The schematic figure was generated based on previous work of phenotypic characterization of the capsule [14], our previous studies of comparative exoproteome analysis [15], and in the results of this work. It is remarkable that the 7108 strain possesses a reduced repertoire of proteins related to virulence compared to the ATCC 15305 and 9325 strains.…”

“…For example, the highly capsular 9325 strain secretes higher content of antigenic proteins and transglycosylases while the non-capsular 7108 strain does not secrete the SsaA antigenic protein and secretes higher content of proteases. These results show diversity in protein secretion among strains [15].In our work, we used a proteomic approach to compare three S. saprophyticus strains possessing different patterns of the capsule: the reference strain ATCC 15305 possessing capsule, the non-capsular 7108 strain, and the highly capsular strain 9325. Of special note, ATCC 15305 produces higher content of proteins for purine biosynthesis.…”