The Examination of the Influence of Caffeinated Coffee Consumption on the Concentrations of Serum Prolactin and Selected Parameters of the Oxidative-Antioxidant Balance in Young Adults: A Preliminary Report

Abstract

We verified whether caffeinated coffee consumption influenced the concentrations of prolactin (PRL) and oxidative stress parameters: total antioxidant status (TAS), ferric reducing antioxidant power (FRAP), total oxidant status (TOS), oxidative stress index (OSI), advanced oxidation protein products (AOPP), uric acid (UA), total bilirubin (T-Bil), albumin (ALB), iron (Fe), calcium (Ca), magnesium (Mg), and inflammatory marker C-reactive protein (CRP)—in blood sera obtained at 15, 60, and 120 minutes after caffeinated coffee intake, in relation to the fasting point. The study participants were 33 young, healthy, nonsmoking volunteers (15 men, 18 women) aged 19-29 years. PRL concentrations significantly decreased ( p < 0.05 ) after consumption, except at time point 15’ in men ( p > 0.05 ). In women, FRAP levels significantly increased over time, and significant changes were also observed for UA at 120’ and ALB at 15’. In men, significant changes were found for levels of AOPP at 15’, T-Bil and ALB at 15’, iron at 60’ and 120’, and calcium at 120’. There were no significant differences in the levels of other examined parameters between the defined time points. In conclusion, the substances contained in caffeinated coffee decrease the level of prolactin and may also have an impact on selected parameters of oxidative stress, which could be the basis of future research focused on the identification of new therapeutic targets.

Effect of prolactin on cytotoxicity and oxidative stress in ovine ovarian granulosa cells

“…Previous study showed that a moderate dose of PRL promotes the antioxidant capacity of adult RPE 19 human cells by reducing glutathione ( Melendez Garcia et al, 2016 ), suggesting PRL could exert an antioxidant action. However, several studies have discovered that HPC leads to oxidative stress in mammals ( Farmer, Lapointe & Cormier, 2017 ), with an increase in ROS generation and alterations to antioxidant system components ( Rodak et al, 2022 ), which is consistent with the results of the present study. In our study, the level of ROS in the four ng/mL group was decreased, the content of MDA depressed, and the levels of SOD and T-AOC were increased compared to other groups, but the results of the 500 ng/ml group showed the opposite trend.…”

“…In our study, the low concentration (four ng/mL) of PRL promoted cell proliferation and inhibited cytotoxicity, while HPC showed the opposite effect in ovine ovarian GCs, which was consistent with the previous study. Additionally, PRL has also been reported to play an important role in oxidative-antioxidant balance ( Farmer, Lapointe & Cormier, 2017 ; Rodak et al, 2022 ). Previous study showed that a moderate dose of PRL promotes the antioxidant capacity of adult RPE 19 human cells by reducing glutathione ( Melendez Garcia et al, 2016 ), suggesting PRL could exert an antioxidant action.…”

“…A previous study has shown that 10 ng/mL PRL can promote endothelial cell proliferation and capillary formation ( Malaguarnera et al, 2002 ), whereas 2 µM PRL dramatically increased the NK-mediated killing of the K562 cell line ( Mavoungou, Bouyou-Akotet & Kremsner, 2005 ), indicating that low concentrations of PRL promote cell proliferation, but high concentrations increase cytotoxicity. Some studies also emphasized that different doses of PRL can alter oxidative-antioxidant balance in mammals ( Farmer, Lapointe & Cormier, 2017 ; Rodak et al, 2022 ). Thebanlt (2017) showed that PRL helps the retinal pigment epithelium to survive via antioxidant actions, while Rodak et al (2022) found that treatment with 300 ng/mL of PRL induced oxidative stress in human MIN6 cells.…”

“…Some studies also emphasized that different doses of PRL can alter oxidative-antioxidant balance in mammals ( Farmer, Lapointe & Cormier, 2017 ; Rodak et al, 2022 ). Thebanlt (2017) showed that PRL helps the retinal pigment epithelium to survive via antioxidant actions, while Rodak et al (2022) found that treatment with 300 ng/mL of PRL induced oxidative stress in human MIN6 cells. PRL excess (hyperprolactinemia) may lead to hypogonadism, which can aggravate the oxidative stress in the body ( Veena et al, 2008 ).…”

Physical Activity Combined with Tea Consumption could further Reduce All-cause Mortality: Results from the US National Health and Nutrition Examination Survey, 2009-2018

“…Siener et al conducted experiments on ten men between the ages of 20 and 31 and found that the consumption of 1.5 L/day of black tea resulted in a signi cant increase in urinary citrate excretion by 21% over the 24-h urine collection period, which is an important inhibitor of calcium stone formation (30). Whereas in the study by Rodak et al, the substances contained in caffeinated coffee decrease the level of prolactin, which is an important hormone for women with more than 300 described functions, including regulation of reproductive function, the immune system, osmotic balance, and angiogenesis (31). This also suggests us that we should do more research on the caffeine contained in tea.…”