The Evolution of Plant Secretory Structures and Emergence of Terpenoid Chemical Diversity

Lange, B. Markus

doi:10.1146/annurev-arplant-043014-114639

Cited by 158 publications

(116 citation statements)

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“…lavender, sage). Some metabolites also have pharmacological properties such as artemisinin, an antimalaria agent secreted by Artemisia annua trichomes (Tissier, 2012;Glas et al, 2012;Lange and Turner, 2013;Lange, 2015;Wagner, 1991).…”

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confidence: 99%

Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity

et al. 2017

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ORCID IDs: 0000-0003-1551-4699 (M.P.); 0000-0002-2315-6900 (M.B.).Ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) is the most abundant enzyme in plants and is responsible for CO 2 fixation during photosynthesis. This enzyme is assembled from eight large subunits (RbcL) encoded by a single chloroplast gene and eight small subunits (RbcS) encoded by a nuclear gene family. Rubisco is primarily found in the chloroplasts of mesophyll (C3 plants), bundlesheath (C4 plants), and guard cells. In certain species, photosynthesis also takes place in the secretory cells of glandular trichomes, which are epidermal outgrowths (hairs) involved in the secretion of specialized metabolites. However, photosynthesis and, in particular, Rubisco have not been characterized in trichomes. Here, we show that tobacco (Nicotiana tabacum) trichomes contain a specific Rubisco small subunit, NtRbcS-T, which belongs to an uncharacterized phylogenetic cluster (T). This cluster contains RbcS from at least 33 species, including monocots, many of which are known to possess glandular trichomes. Cluster T is distinct from the cluster M, which includes the abundant, functionally characterized RbcS isoforms expressed in mesophyll or bundle-sheath cells. Expression of NtRbcS-T in Chlamydomonas reinhardtii and purification of the full Rubisco complex showed that this isoform conferred higher V max and K m values as well as higher acidic pH-dependent activity than NtRbcS-M, an isoform expressed in the mesophyll. This observation was confirmed with trichome extracts. These data show that an ancient divergence allowed for the emergence of a so-far-uncharacterized RbcS cluster. We propose that secretory trichomes have a particular Rubisco uniquely adapted to secretory cells where CO 2 is released by the active specialized metabolism.

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Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity

et al. 2017

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“…It has been reported that the primary function of secretory structures is related to defense responses against both herbivores and pathogens. (Fahn, 1988;Lange, 2015). These structures have the ability to produce or sequester secondary (or specialized) metabolites.…”

Section: Secretory Structuresmentioning

confidence: 99%

A Comprehensive Review on the Genus Plumbago With Focus on Plumbago Auriculata (Plumbaginaceae)

Singh¹,

Naidoo²,

Baijnath³

2017

Afr J Tradit Complement Altern Med

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Background: The genus Plumbago distributed in warm tropical regions throughout the world is the largest genus in Plumbaginaceae. Medicinal plants are characteristic to the genus Plumbago and are cultivated and utilized worldwide. Plumbago auriculata Lam. is common in South Africa and is often cultivated for its ornamental and medicinal uses throughout the world. Materials and Methods: A comprehensive review of the genus Plumbago with focus on Plumbago auriculata was carried out and information was gathered using scientific publications, conference proceedings, the internet and books. Articles based on the morphology, pharmacological and medicinal uses of Plumbago auriculata was analysed thoroughly. Results: Plumbago auriculata plant parts posses a wide range of phytochemicals with plumbagin being the marker compound showing various pharmacological activities. Different plant parts are claimed to be used for the treatment of human and animal ailments, however they do exhibit toxic properties and need to be administered with caution. Salt secreting glands and trichomes are characteristic of Plumbaginaceae. Conclusion: This study reveals new insights on the genus Plumbago and the potential use of species in the genus as medicinal plants. Plumbago auriculata possess the bioactive compound plumbagin and secondary metabolites, thus, it is of high medicinal importance. P. auriculata is a poorly nor favourite studied species in the genus Plumbago and further research needs to be carried out to explore specific details of the species.

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“…The class I terpene synthases (TPSs) are classified as mono-, sesqui-and diterpene synthases according to their catalytically preferred substrates geranyl diphosphate, farnesyl diphosphate and geranylgeranyl diphosphate, respectively. Terpene synthase-6 (TPS-6) is a kind of sesqui terpenes (Lange 2015). Characterization and genetically identification of various terpene synthases from different aromatic plants in future will provide important advantages in increasing of terpene production in industry.…”

Section: Introductionmentioning

confidence: 99%

PARTIAL CLONING AND IDENTIFICATION OF TERPENE SYNTHASE-6 GENE (TPS-6) IN AN AROMATIC PLANT Origanum onites L.

Poyraz¹,

Sağlam²

2017

Trakya University Journal of Natural Sciences

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Origanum onites L. (oregano) is an aromatic plant used in medicine, cosmetics and different industrial fields. It includes essential oil and is extensively cultivated as aromatic plant in the world. Therefore, it is also preferred as a spice in foods. Terpene synthase-6 (TPS-6) is an enzyme responsible for synthesis of sesqui-terpenes in aromatic plants. The investigation of gene sequence information and expression characteristics of this enzyme in O. onites may contribute to increase of synthesis of terpenes as raw materials in medicine and cosmetic etc. industry. In this study, partial cloning and sequencing of the TPS-6 gene in the O. onites was aimed. Total RNA isolation from O. onites was performed with TRI Reagent solution. The mRNAs were transformed to cDNAs by Revers Transcription Method. The middle region of TPS-6 gene was amplified by PCR method using degenerative primers designed for TPS-6. Amplified DNA fragments were obtained at 481bp. size and ligated to PGEM T Easy plasmid vector with T4 DNA ligase. The recombinant plasmid vector was transferred to the XL1-Blue host cells (E. coli) and the cloned DNA was sequenced. The partial TPS-6 DNA sequence was confirmed in NCBI GenBank database and registered with an accession number (MF983853). The obtained DNA sequence was analyzed with InterProScan and Clustal Omega software in EBI database. The analysis results showed that this DNA sequence has a specific motif (DXXD) for TPS-6 catalyzing cyclization reactions. The phylogenetic dendrogram was drawn for comparison with other plant TPS-6 genes. O. onites TPS-6 sequence was determined to be very close to O. vulgare TPS-6 on the dendrogram than other plant TPS-6s. The obtained gene sequence information will guide biotechnological researches in the productivity increase of this gene using expression analysis.

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The Evolution of Plant Secretory Structures and Emergence of Terpenoid Chemical Diversity

Cited by 158 publications

References 91 publications

Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity

Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity

A Comprehensive Review on the Genus Plumbago With Focus on Plumbago Auriculata (Plumbaginaceae)

PARTIAL CLONING AND IDENTIFICATION OF TERPENE SYNTHASE-6 GENE (TPS-6) IN AN AROMATIC PLANT Origanum onites L.

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