The evaluation of zebrafish cardiovascular and behavioral functions through microfluidics

Subendran, Satishkumar; Wang, Yi-Chieh; Lu, Yueh‐Hsun; Chen, Chia Yuan

doi:10.1038/s41598-021-93078-y

Cited by 10 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chakraborty et al ( 2011 ) observed that in zebrafish eleutheroembryos, an increase in heart rate induced by CAF (10, 20, and 50 mg.L −1 ) at 72 hpf is associated with a decrease in the expression of vascular endothelial growth factor- a protein involved in the development of heart blood vessels and endothelial muscle development, which results in abnormalities in the heart or in the development of cardiac blood vessels and, consequently, increased heart rate. Contrary to our findings, Subendran et al ( 2021 ) observed a decrease in the heart rate of zebrafish larvae at 5, 7, and 9 dpf when exposed, for 10 min, to 25 mg.L −1 of CAF. The authors suggest that CAF effects on zebrafish heart rate are due to the release of calcium in the cytoplasm, which could lead to cardiac nerve transmission and, consequently, a reduction in heart rate.…”

Section: Discussioncontrasting

confidence: 99%

Influence of exposure scenario on the sensitivity to caffeine

Santos,

Oliveira,

Domingues

2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

The chorion acts as a protective barrier, restricting some chemical absorption into the embryo and the surrounding fluids. In this sense, larvae may only have direct contact with some chemicals after dechorionation. This study aimed to evaluate the effects of caffeine (CAF) (0, 13, 20, 44, 67, and 100 mg.L−1) under different exposure scenarios (embryos with chorion or embryos/larvae already hatched) and rank the stage sensitivity. Thus, three scenarios were investigated: from 2 to 120 hours post fertilization (hpf) (5 days of exposure- 5dE), from 72 to 120 hpf (2dE), and from 96 to 120 hpf (1dE). Heart rate (48 hpf) and energy reserves (120 hpf) were measured in the 5dE scenario, and behavior and acetylcholinesterase (AChE) activity were evaluated at 120 hpf in all scenarios (5dE, 2dE, and 1dE). At 120 hpf, some of the fish was transferred to clean medium for a 10 days depuration period (10dPE). Behavior and AChE activity were assessed after this period. In the 5dE scenario, CAF increased heartbeat (13, 20, and 30 mg.L−1) and reduced carbohydrates (67, and 100 mg.L−1), while inhibiting AChE activity (100 mg.L−1) in the 5dE, 2dE, and 1dE scenarios. CAF reduced the total distance moved in the 5dE (67, and 100 mg.L−1), 2dE (20, 30, 44, 67, and 100 mg.L−1), and 1dE fish (67, and 100 mg.L−1) and increased erratic movements. Based on the lowest observed effect concentration (LOEC) for total distance moved (20 mg.L−1) and higher inhibition of AChE activity (100 mg.L−1) (65%), 2dE fish appear to be more sensitive to CAF. After 10dPE, a recovery in behavior was detected in all scenarios (5dE, 2dE, and 1dE). AChE activity remained inhibited in the 2dE scenario while increasing in the 1dE scenario. This study demonstrated that the presence of the chorion is an important factor for the analysis of CAF toxicity. After the loss of the chorion, organisms show greater sensitivity to CAF and can be used to evaluate the toxicity of various substances, including nanomaterials or chemicals with low capacity to cross the chorion. Therefore, the use of hatched embryos in toxicity tests is suggested, as they allow a shorter and less expensive exposure scenario that provides similar outcome as the conventional scenario.

show abstract

Section: Discussioncontrasting

confidence: 99%

Influence of exposure scenario on the sensitivity to caffeine

Santos,

Oliveira,

Domingues

2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

show abstract

“…A sustained increase in heart rate was also induced by a chemical irritant (Roberts et al, 2020). Heart rate can be measured via imaging larvae embedded in agarose (Mann et al, 2010) while more complex features such as blood flow velocity and circulation can be assessed concurrently with behavioral responses (fin beating) using microfluidic set-ups (Subendran et al, 2021). Another biological endophenotype is cortisol levels that can be assayed via radioimmunoassays (Steenbergen et al, 2012) or enzymelinked immunosorbent assays (ELISAs) (Yeh et al, 2013).…”

Section: Open Questions Challenges and Future Prospectsmentioning

confidence: 99%

Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Tan

Ang

Wee

2022

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Animal models are essential for the discovery of mechanisms and treatments for neuropsychiatric disorders. However, complex mental health disorders such as depression and anxiety are difficult to fully recapitulate in these models. Borrowing from the field of psychiatric genetics, we reiterate the framework of ‘endophenotypes’ – biological or behavioral markers with cellular, molecular or genetic underpinnings – to reduce complex disorders into measurable behaviors that can be compared across organisms. Zebrafish are popular disease models due to the conserved genetic, physiological and anatomical pathways between zebrafish and humans. Adult zebrafish, which display more sophisticated behaviors and cognition, have long been used to model psychiatric disorders. However, larvae (up to 1 month old) are more numerous and also optically transparent, and hence are particularly suited for high-throughput screening and brain-wide neural circuit imaging. A number of behavioral assays have been developed to quantify neuropsychiatric phenomena in larval zebrafish. Here, we will review these assays and the current knowledge regarding the underlying mechanisms of their behavioral readouts. We will also discuss the existing evidence linking larval zebrafish behavior to specific human behavioral traits and how the endophenotype framework can be applied. Importantly, many of the endophenotypes we review do not solely define a diseased state but could manifest as a spectrum across the general population. As such, we make the case for larval zebrafish as a promising model for extending our understanding of population mental health, and for identifying novel therapeutics and interventions with broad impact.

show abstract

“…18 Due to accelerating multifaceted learning and memory paradigms developed for adult zebrafish, 19 researchers have extended their studies on larval zebrafish using tiny well-plates, 20,21 petri dishes, 22 and microfluidic devices. 23–29…”

Section: Introductionmentioning

confidence: 99%