The Estimation of Heparin and Similar Substances in Human Blood and Tissues Using a Combined Biological and Colorimetric Method with Paper Electrophoretic Studies

The Distribution of Acid Mucopolysaccharides in Cohn Fractions of Human Plasma Proteins1

“…Acid mucopolysaccharides behaving similarly to chondroitin sulfate on paper electrophoresis and paper chromatography have been demonstrated previously in human leukocytes (1,2). A chondroitin sulfate-like substance has been recovered also from blood platelets of rats (3).…”

The Occurrence of Acid Mucopolysaccharides in Human Leukocytes and Urine 1

“…of 5 per cent sodium citrate in a balanced salt solution. 2 The red blood cells were sedimented rapidly by mixing 2.5 ml. of 5 per cent dextran with the blood in each tube.…”

“…Bassiouni (2) recently isolated a substance from leukocytes which he noted to be similar to chondroitin sulfate. In our laboratory leukocytes have been found to contain appreciable quantities of a substance resembling chondroitin sulfate.…”

Studies of the Acid Polysaccharide of the White Cells in Rheumatic and Other Diseases showing its similarity to the Acid Polysaccharide of Amyloid

“…In a previous investigation (Bassiouni, 1954) injected heparin was isolated from human blood and tissues and endogenous acid polysaccharides from various tissues. The method is summarized in Fig.…”

“…* As the concentration of the A.P.S. in such a fine precipitate would be very low, extraction and purification by the usual procedure (step G in the diagram) would entail very considerable losses (Bassiouni, 1954) and leave insufficient material for final estimation. In order to avoid these losses a procedure modified especially for use with small samples was adopted, in which the crude complex dye precipitate obtained in step F was dissolved in 002 ml.…”