The Distribution of Acid Mucopolysaccharides in Cohn Fractions of Human Plasma Proteins1

The glycosaminoglycans of human plasma

“…These results are in good agreement with those obtained by previous authors (3)(4)(5)(6) and confirm that chondroitin-4-sulfate is the main glycosaminoglycan of normal human plasma. The reported presence of components resistant to the action of testicular hyaluronidase (6) may be due to the small amounts of heparan sulfate and keratan sulfate demonstrated, or to the possible presence of excessive amounts of protein in some crude preparations.…”

“…However, a fraction of plasma GAG cannot be retained on, ECTEOLA before proteolytic digestion, and it is likely that this fraction is "bound" to protein through bonds other than electrostatic. Evidence provided by previous authors (2,5,6,22) seems to indicate that at least a part of plasma GAG may be covalently linked to proteins. The possibility that the "bound" GAG may be linked to plasma proteins by a larger number of electrostatic bonds does not seem to be supported by the results of the fractionation on Dowex 1 X 2 columns, nor by the analytical and infrared data.…”

Histochemical studies on glycosaminoglycans of odontogenic cysts

“…Toller (3) suggested a somewhat simplistic view that cyst fluid was a simple dialysate of blood, whieh passed through a 'semi-permeable' eyst lining into the lumen. However, the levels of free glyeosaminoglyeans in serum (5)(6)(7)(8) are up to 1000 times less than those found in eyst fluids. Although a coneentrating effect where certain molecules may selectively pass into the cyst lumen could be suggested, it is unlikely as other serum constituents, e.g.…”

The Effect of Inflammation on the Hexuronate-Containing Polysaccharides of Human Plasma1

“…In view of the probable identity of these polysaccharides with some components of ground substance (2)(3)(4), this is an observation of considerable interest. If it should apply only to patients with rheumatoid arthritis, it might be a reflection of basic changes in the metabolism of connective tissue in that disease.…”