The Estimation of Heat and Drought Tolerance Maize Lines Using Dna Markers

Abstract

The main factor which causes to decrease maize grain yield is drought. In most regions where maize is grown, the water stress during the growing period is caused by both lack of soil moisture and high air temperature. The purposes of our study were the estimation and selection of maize lines for drought and heat tolerance based on DNA markers and determination of the correlation between CAPS markers and plant ability to resist the water stress. As the result of study, the significant differences were found between leaf temperature of maize lines which contained favorable alleles by both CAPS markers in 2018 and 2019 (35.72 and 34.41ºC respectively), LSD0.05=1.27. The leaf temperature of maize lines which had SNP (A) by dhn С397 (36.95ºC) differenced significantly with lines contained favorable allele by rspC1090 or lines with no favorable alleles in 2018 (33.68 and 34.35ºC respectively). Based on analysis by seeds germinating in sucrose solution the significant differences were observed between the amount of sprouted seeds in lines contained SNP(G) by rspC1090 and lines without any favorable allele (4% and 2.25% respectively), LSD0.05= 1.70. As the result of correlation analysis, the positive correlation was determined between SNP(A) by dhnС397 marker in maize lines and leaf temperature in 2018-2019 (r=0.16). The positive correlation was observed between SNP(G)byrspC1090 and the percent of sprouted seeds in sucrose solution (r=0.31). Thus, for complex estimation and maize line selection for drought and heat tolerance it could be recommended to use two CAPS markers dhnC397 and rspC1090.

Mapping of yield contributing drought tolerance QTLs in maize and its utilization in marker-assisted breeding

“…Prysiazhniuk et al (2018) found the two genes dhn1 and rsp41to be related with drought resistance in maize lines. Prysiazhniuk et al (2018) suggested that these lines can be used further for development of drought-resistant maize hybrids. As in present case, about 51 plants for LM23 and 89 for LM24 were found in BC 3 F 1 .…”