The widespread occurrence of coccidiostat resistance in chicken farms has prompted the search for alternatives to treat coccidiosis caused by Eimeria tenella. This study aimed to compare the efficacy of standard artemisinin and Artemisia annua (A. annua) leaf ether extract when administered at 3-2-3 intervals to chickens with coccidiosis. A total of 30 Cobb strain roosters were infected with E. tenella at a dose of 2000 oocysts and randomly assigned to six treatments with five replications, including the group without treatment (PI), the group treated with Sulfa/commercial drug (P II), and the groups treated with 8.5 ppm (P III), 17 ppm (P IV), 34 ppm (P V) and A. annua leaf extract (P VI). Five hens that were not infected with E. tenella comprised a separate treatment (P 0). The oral treatment was administered on a 3 -2 -3 schedules consisting of three consecutive days of treatment, a two-day break, and then three consecutive days of treatment. Variables examined included clinical symptoms, number of oocysts in faeces, body weight growth, cecum damage score, PCV and haemoglobin (Hb) values. The result revealed that the first detection of E. tenella oocysts in faeces occurred five days after infection. The group treated with 17 ppm A. annua leaf extract had the greatest reduction in oocysts (P VI), while there was no significant difference in body weight (P > 0.05) between this group and the group treated with a commercial drug (P II). The administration of standard artemisinin and A. annua leaf extract significantly reduced cecum damage (P < 0.05) compared to the untreated group (P I). Hb values in all treatment groups were considered normal, whereas only the negative control and commercial coccidiostats groups exhibited abnormal PCV values. An alternative coccidiostats may be substituted with artemisinin and ether extract of A. annua leaf in chickens infected with E. tenella.