The Essentialness of Delignification on Enzymatic Hydrolysis of Sugar Cane Bagasse Cellulignin for Second Generation Ethanol Production

Barcelos, Carolina Araújo; Maeda, Roberto Nobuyuki; Betancur, Gabriel Jaime Vargas; Pereira, Nei

doi:10.1007/s12649-012-9137-3

Cited by 27 publications

(16 citation statements)

References 19 publications

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“…These contents were lower than those observed by Agblevor et al (2006), who reported a wide variation in the ash content (10.8-21.9%) for samples derived from four ginning processes. The ash content in CGD and CGW was also lower than what is found in other biomass types such as rice straw (17.5%) and wheat straw (11.0%) ( Barcelos et al, 2013), while industrial samples of sugarcane bagasse (6.5%) and straw (6.2%) (Szczerbowski et al, 2014) contained similar amounts of inorganic compounds.…”

Section: Cgd and Cgw Chemical Compositionmentioning

confidence: 64%

Production of cellulosic ethanol from cotton processing residues after pretreatment with dilute sodium hydroxide and enzymatic hydrolysis

Fockink

Maceno

Ramos

2015

Bioresource Technology

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Section: Cgd and Cgw Chemical Compositionmentioning

confidence: 64%

Production of cellulosic ethanol from cotton processing residues after pretreatment with dilute sodium hydroxide and enzymatic hydrolysis

Fockink

Maceno

Ramos

2015

Bioresource Technology

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“…This partial delignification is essential for the 219 subsequent enzymatic hydrolysis, because it improves the accessibility 220 of the enzymes to cellulose [7]. Furthermore, lignin limits the rate and 221 the extent of enzymatic hydrolysis by acting as a "shield", preventing 222 the digestible parts of the substrate to be hydrolyzed [12].…”

mentioning

confidence: 99%

“…Delignification allows a swelling of 228 the fibers and breaks the structural association between lignin and 229 carbohydrates [7]. [7,[17][18][19].…”

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confidence: 99%

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Potential of giant reed (Arundo donax L.) for second generation ethanol production

Silva

Schirmer

Maeda

et al. 2015

Electronic Journal of Biotechnology

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“…The liquid containing the hemicellulosic hydrolysate is separated from the solid fraction, which mainly consists of cellulose and lignin, i.e., cellulignin. A partial delignification stage of this solid fraction has been shown to be essential to increase the accessibility of the enzymes to the cellulose fibers [3], resulting in hydrolysates containing high glucose concentrations.…”

Section: Introductionmentioning

confidence: 99%

METABOLIC ADAPTATION STRATEGY USING Zymomonas mobilis CP4 CELLS FOR THE PRODUCTION OF SECOND GENERATION ETHANOL

Martins

Borges

Reis³

et al. 2015

JBT

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During the past few decades, considerable effort has been made to utilize agricultural and forest residues as biomass feedstock for the production of bioethanol as an alternative fuel. The bacterium Zymomonas mobilis was shown to be extremely attractive for the production of second-generation ethanol from glucose of the cellulose fraction due to its ability to uptake high amounts of this sugar, resulting in high ethanol productivity values. However, the wild-type strains are unable to metabolize xylose that arises from the hemicellulose fraction. Molecular biology techniques were incorporated to render the strain used in this study capable of fermenting xylose into ethanol and thus increase the efficiency of secondgeneration ethanol production. Thus, the aim of this study was to evaluate the performance of a recombinant strain of Z. mobilis in simultaneous saccharification and co-fermentation (SSCF) processes, in which the fermentation of both sugars (glucose and xylose) occurs in one step. Regarding the genetic transformation,the 1,565 kb Z. mobilis plasmid pZMO1 was chemically synthesized and cloned into a synthetic vector that contains the E. coli and Z. mobilis replication checkmark origin,the XI, XK, TAL, and TKL genes and tetracycline resistance. Metabolic adaptation was performed by transferring the recombinant strain to media containing increased xylose concentrations. Then, an experimental response surface methodology was used to evaluatethe addition of glucose and xylose with different concentrations, as well as the incorporation of hemicellulosic hydrolyzate in different proportions. The recombinant Z. mobilis CP4 strain reached 25 g/L ethanol, confirming that approximately 50% of this pentose was consumed in the SSCF process when using 30% solids, 20.5% hemicellulose hydrolysate, 10 mg/L tetracycline, an enzyme load of 25 FPU/g cellulignin, and 10% of the initial inoculum.

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The Essentialness of Delignification on Enzymatic Hydrolysis of Sugar Cane Bagasse Cellulignin for Second Generation Ethanol Production

Cited by 27 publications

References 19 publications

Production of cellulosic ethanol from cotton processing residues after pretreatment with dilute sodium hydroxide and enzymatic hydrolysis

Production of cellulosic ethanol from cotton processing residues after pretreatment with dilute sodium hydroxide and enzymatic hydrolysis

Potential of giant reed (Arundo donax L.) for second generation ethanol production

METABOLIC ADAPTATION STRATEGY USING Zymomonas mobilis CP4 CELLS FOR THE PRODUCTION OF SECOND GENERATION ETHANOL

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