The Epididymis

Robaire, Bernard; Hinton, Barry T.

doi:10.1016/b978-0-12-397175-3.00017-x

Cited by 103 publications

(164 citation statements)

References 1,057 publications

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“…Almost all male CF patients are diagnosed with congenital bilateral absence of the vas deferens (CBAVD) and concurrent absence or atrophy of a large portion of the epididymis (Anguiano et al, 1992;Cuppens and Cassiman, 2004;Oates and Amos, 1994;Patrizio and Zielenski, 1996;Yu et al, 2012). These organs are located downstream of the testis; they are required for sperm maturation, storage and transport, and are thus essential for the establishment of male fertility (Chen et al, 2012b;Cornwall, 2009;Robaire et al, 2006;Shum et al, 2011). In addition, men carrying silent mutations of CFTR that do not cause CF have reduced fertility while being otherwise healthy, indicating that the male reproductive organs are among the most affected by these mutations (Chan et al, 2009;Cuppens and Cassiman, 2004;Schulz et al, 2006;van der Ven et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation via the ZONAB pathway

Ruan

Wang

Silva

et al. 2014

Journal of Cell Science

102

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Mutations in CFTR lead to dysfunction of tubular organs, which is currently attributed to impairment of its conductive properties. We now show that CFTR regulates tight junction assembly and epithelial cell differentiation through modulation of the ZO-1-ZONAB pathway. CFTR colocalizes with ZO-1 at the tight junctions of trachea and epididymis, and is expressed before ZO-1 in Wolffian ducts. CFTR interacts with ZO-1 through the CTFR PDZ-binding domain. In a three-dimensional (3D) epithelial cell culture model, CFTR regulates tight junction assembly and is required for tubulogenesis. CFTR inhibition or knockdown reduces ZO-1 expression and induces the translocation of the transcription factor ZONAB (also known as YBX3) from tight junctions to the nucleus, followed by upregulation of the transcription of CCND1 and downregulation of ErbB2 transcription. The epididymal tubules of cftr 2/2 and cftr DF508 mice have reduced ZO-1 levels, increased ZONAB nuclear expression, and decreased epithelial cell differentiation, illustrated by the reduced expression of apical AQP9 and V-ATPase. This study provides a new paradigm for the etiology of diseases associated with CFTR mutations, including cystic fibrosis.

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Section: Introductionmentioning

confidence: 99%

CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation via the ZONAB pathway

Ruan

Wang

Silva

et al. 2014

Journal of Cell Science

102

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“…The epididymis has a ductal structure and is consisted of a lay of epithelial cells surrounded by smooth muscle layers (Robaire & Hermo, 1988). The epididymal epithelium possesses several cell types, including principal, narrow, basal, halo, and apical cell (Robaire & Hermo, 1988). Each cell type in the epididymal epithelium has different morphologies and functions (Robaire et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Lee¹

2016

Development &Amp; Reproduction

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Direct communication between neighboring cells via gap junction in tissue is important for maintenance and regulation of its physiological functions. Each epididymal region has different composition of cell types. It is well recognized that the epididymis is a steroid hormone-responsive tissue. The present study was designed to determine the effect of estradiol benzoate (EB) or flutamide exposured at the early postnatal age on the expression of connexin (Cx) isoforms in the caudal epididymis. The EB or flutamide was subcutaneously administrated to male Spragure Dawley rat at 7 days of age, and expressional changes of Cx isoforms in the adult corpus epididymis were determined by quantitative real-time PCR. The treatment of low-dose EB resulted in decreases of Cx30.3, Cx31.1, Cx37, and Cx45 expression but caused an increase of Cx32 expression. Exposure to high-dose EB led into expressional increases of Cx31, Cx31.1, Cx32, Cx40, and Cx43, even though a decrease of Cx37 expression was found with a high-dose EB treatment. A low-dose flutamide induced increases of Cx31, Cx31.1, Cx32, and Cx43 expression but a decrease of Cx37 expression. Expression of most Cx genes were significantly increased by a high-dose flutamide, while no expressional change of Cx26 and Cx40 was detected by a high-dose flutamide. These results indicate that expression of Cx isoforms in the caudal epididymis is altered by exposure to steroidal compounds at the prepubertal age. It is suggested that a contact with environmental exogenous materials during the early postnatal period would lead to alteration of epididymal functions at the adult.

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“…The epididymis is a part of the excurrent duct of male reproductive tract and is the place in which sperm becomes acquiring fertilizing capacity (Robaire & Hermo, 1988). The epididymis is structurally and functionally separated into 4 different regions, including initial segment, caput epididymis, corpus epididymis, and caudal epididymis (Robaire & Hermo, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…The epididymis is structurally and functionally separated into 4 different regions, including initial segment, caput epididymis, corpus epididymis, and caudal epididymis (Robaire & Hermo, 1988). Among these regions of the epididymis, the caudal epididymis plays an important role for storage and quiescence of mature sperm (Robaire & Hermo, 1988). Thus, it is fully reasonable to consider that functional regulation of the caudal epididymis could give an influence on the male reproductive ability.…”

Section: Introductionmentioning

confidence: 99%

Effects of Exposure to Estradiol Benzoate or Flutamide at the Weaning Age on Expression of Connexins in the Caudal Epididymis of Adult Rat

Lee

2016

Dev Reprod

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The present research was chiefly designed to determine the effect of the treatment of estrogenic agonist, estradiol benzoate (EB), or antiandrogenic compound, flutamide (Flu), at the weaning age on the expression of connexin (Cx) isoforms in the caudal epididymis of adult male rat. Animals were subcutaneously administrated with a single shot of either EB at a low-dose (0.015 µg of EB/kg body weight (BW)) or a high-dose (1.5 µg of EB/kg BW) or Flu at a low-dose (500 µg of EB/kg BW) or a high-dose (5 mg of EB/kg BW). Expressional changes of Cx isoforms in the adult caudal epididymis were examined by quantitative real-time PCR analysis. The treatment of a low-dose EB caused significant increases of Cx30.3, Cx31, Cx32, and Cx43 transcript levels but reduction of Cx31.1, Cx37, and Cx45 expression. Exposure to a high-dose EB resulted in very close responses observed in a low-dose EB treatment, except no significant expressional change of Cx37 and a significant induction of Cx40. Expression of all Cx isoforms, except Cx45, was significantly increased by a low-dose Flu treatment. Expressional increases of all Cx isoforms were detected by a high-dose Flu treatment. The current study demonstrates that a single exposure to estrogenic or antiandrogenic compound during the early postnatal developmental period is sufficient to disrupt normal expression of Cx isoforms in the adult caudal epididymis.

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The Epididymis

Cited by 103 publications

References 1,057 publications

CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation via the ZONAB pathway

CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation via the ZONAB pathway

Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age

Effects of Exposure to Estradiol Benzoate or Flutamide at the Weaning Age on Expression of Connexins in the Caudal Epididymis of Adult Rat

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