The environmental quality of Doñana surrounding areas affects the immune transcriptional profile of inhabitant crayfish Procambarus clarkii

Osuna-Jiménez, Inmaculada; Abril, Nieves; Vioque-Fernández, Amalia; Gómez-Ariza, José Luis; Prieto‐Álamo, María‐José; Pueyo, Carmen

doi:10.1016/j.fsi.2014.06.031

Cited by 19 publications

(3 citation statements)

References 61 publications

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“…Three typical domains-α-helices in the N-terminal first domain, two copper-binding sites containing six histidine residues in the second domain, and β-sheets in the third domain (immunoglobulin-like 42 ZENG domain)-were also found in both subunits. Even though the partial sequences of the two hemocyanin subunits PcHc1 and PcHc2, named HC and HC2, respectively, have been identified by suppression subtractive hybridization, they were not available when our project was initiated (Osuna-Jiménez et al 2014). A positive and significant linear correlation found between the hepatopancreatic copper concentration and the levels of the HEMOCYANIN IN RED SWAMP CRAYFISH transcripts encoding hemocyanins and the expression levels of mRNA of the hemocyanin subunits could be used as molecular biomarkers in environmental monitoring (Osuna-Jiménez et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Even though the partial sequences of the two hemocyanin subunits PcHc1 and PcHc2, named HC and HC2, respectively, have been identified by suppression subtractive hybridization, they were not available when our project was initiated (Osuna-Jiménez et al 2014). A positive and significant linear correlation found between the hepatopancreatic copper concentration and the levels of the HEMOCYANIN IN RED SWAMP CRAYFISH transcripts encoding hemocyanins and the expression levels of mRNA of the hemocyanin subunits could be used as molecular biomarkers in environmental monitoring (Osuna-Jiménez et al 2014). There have been no previous studies carried out to investigate the responses of red swamp crayfish to a virus challenge up to the present.…”

Section: Discussionmentioning

confidence: 99%

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The cDNA Sequence of Two Hemocyanin Subunits from Red Swamp Crayfish Procambarus clarkii and their Responses to White Spot Syndrome Virus Infection

Zeng

2016

J Aqua Anim Hlth

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Hemocyanin, the respiratory protein of crustaceans, participates in the innate immune defense in these organisms. We cloned two hemocyanin subunit genes (PcHc1 and PcHc2), by using a degenerate primer PCR and rapid amplification of cDNA ends (RACE) approach, from the hepatopancreas of red swamp crayfish Procambarus clarkii. The transcripts of these two subunits were only detected in the hepatopancreas by reverse transcriptase quantitative PCR (RT-qPCR) analysis. The neighbor-joining and maximum parsimony phylogenetic analyses indicated that PcHc2 associated with a clade belong to the α-type hemocyanins and PcHc1 associated with another clade belonging to the β-type hemocyanins. The data obtained from the RT-qPCR indicated that the mRNA expression levels of these subunit genes followed almost the same regulation pattern in the crayfish challenged with white spot syndrome virus (WSSV). The fluctuation of mRNA expression levels of these two subunits after the WSSV challenge indicated that both of them may participate in the antiviral immune response of crayfish. Received April 12, 2015; accepted November 22, 2015.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The cDNA Sequence of Two Hemocyanin Subunits from Red Swamp Crayfish Procambarus clarkii and their Responses to White Spot Syndrome Virus Infection

Zeng

2016

J Aqua Anim Hlth

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“…Procambarus clarkii , the freshwater crayfish is an important commercial species [ 50 , 51 ]. Considering its long lifecycle, wide distribution, and simple anatomy, P. clarkii is often utilized as a typical bioindicator of toxic pollutants in studies on aquatic environments [ 38 , 52 , 53 , 54 ]. Additionally, P. clarkii is considered a model organism for research in aquatic crustaceans [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Mercury Induced Tissue Damage, Redox Metabolism, Ion Transport, Apoptosis, and Intestinal Microbiota Change in Red Swamp Crayfish (Procambarus clarkii): Application of Multi-Omics Analysis in Risk Assessment of Hg

et al. 2022

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As one of the most toxic elements, mercury (Hg) is a widespread toxicant in aquatic environments. Crayfish are considered suitable for indicating the impact of heavy metals on aquatic crustaceans. Nevertheless, Hg toxicity on Procambarus clarkii is largely unknown. In this research, the acute Hg-induced alterations of biochemical responses, histopathology, hepatopancreatic transcriptome, and intestinal microbiome of Procambarus clarkii were studied. Firstly, Hg induced significant changes in reactive oxygen species (ROS) and malonaldehyde (MDA) content as well as antioxidant enzyme activity. Secondly, Hg exposure caused structural damage to the hepatopancreas (e.g., vacuolization of the epithelium and dilatation of the lumen) as well as to the intestines (e.g., dysregulation of lamina epithelialises and extension of lamina proprias). Thirdly, after treatment with three different concentrations of Hg, RNA-seq assays of the hepatopancreas revealed a large number of differentially expressed genes (DEGs) linked to a specific function. Among the DEGs, a lot of redox metabolism- (e.g., ACOX3, SMOX, GPX3, GLO1, and P4HA1), ion transport- (e.g., MICU3, MCTP, PYX, STEAP3, and SLC30A2), drug metabolism- (e.g., HSP70, HSP90A, CYP2L1, and CYP9E2), immune response- (e.g., SMAD4, HDAC1, and DUOX), and apoptosis-related genes (e.g., CTSL, CASP7, and BIRC2) were identified, which suggests that Hg exposure may perturb the redox equilibrium, disrupt the ion homeostasis, weaken immune response and ability, and cause apoptosis. Fourthly, bacterial 16S rRNA gene sequencing showed that Hg exposure decreased bacterial diversity and dysregulated intestinal microbiome composition. At the phylum level, there was a marked decrease in Proteobacteria and an increase in Firmicutes after exposure to high levels of Hg. With regards to genus, abundances of Bacteroides, Dysgonomonas, and Arcobacter were markedly dysregulated after Hg exposures. Our findings elucidate the mechanisms involved in Hg-mediated toxicity in aquatic crustaceans at the tissue, cellular, molecular as well as microbial levels.

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Metabolomic alterations and oxidative stress are associated with environmental pollution in Procambarus clarkii

et al. 2018

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The environmental quality of Doñana surrounding areas affects the immune transcriptional profile of inhabitant crayfish Procambarus clarkii

Cited by 19 publications

References 61 publications

The cDNA Sequence of Two Hemocyanin Subunits from Red Swamp Crayfish Procambarus clarkii and their Responses to White Spot Syndrome Virus Infection

The cDNA Sequence of Two Hemocyanin Subunits from Red Swamp Crayfish Procambarus clarkii and their Responses to White Spot Syndrome Virus Infection

Mercury Induced Tissue Damage, Redox Metabolism, Ion Transport, Apoptosis, and Intestinal Microbiota Change in Red Swamp Crayfish (Procambarus clarkii): Application of Multi-Omics Analysis in Risk Assessment of Hg

Metabolomic alterations and oxidative stress are associated with environmental pollution in Procambarus clarkii

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