The Enterohepatic Circulation of Foreign Bile Acids: The Circulation of Cholates in Hogs With Biliary Fistulae

The Biliary Excretion of Various Bile Acids by Patients With Choledochostomy Drainage, and the Effect of Oral Administration of Desiccated Bile of the Ox and the Hog 1

“…Inasmuch as hyodesoxycholic acid of hog bile is conjugated solely with glycine, this increase in the glyco/ tauro ratio of the drainage bile, together with the excretion of hyodesoxycholic acid, suggests strongly that glycohyodesoxycholic acid was absorbed from the intestine and excreted unchanged in the drainage bile. The efficiency of utilization of this foreign bile acid by these patients appears to be somewhat less than the efficiency with which glycocholic acid was handled by hogs with biliary fistulae (12). However, it is doubtful whether the bile collected by drainage through a T-tube represents the entire biliary secretion even under carefully supervised conditions.…”

“…Cholic acid and total dihydroxycholanic acids 8 were determined by methods which we have described previously (12,13). The method for determining "cholic acid" yields the sum of free cholic acid, glycocholic, and taurocholic acids.…”

“…Hyodesoxycholic acid of hog bile also is a dihydroxycholanic acid (8,15). Our method for the determination of "total dihydroxycholanic acids" (12) yields the sum of these 3 acids.…”

“…802 cedure which we have described previously (12). Optical density measurements were made with a filter photometer at a mean wavelength of 620 my.…”

“…of this acid was extracted and hydrolysed, and the dihydroxycholanic acid fraction was separated by the method previously described (12). The dihydroxycholanic acids, including hyodesoxycholic acids, were oxidized by chromic acid to diketocholanic acids as described previously (12), a proportionately larger amount of chromic acid being required because of the larger sample. The diketocholanic acid fraction was dissolved in glacial acetic acid, and the optical rotation (D line of sodium) was determined in a polarimeter equipped with a micro tube of 1 decimeter length requiring 1 ml.…”