The energy-yielding reactions of Peptococcus pr�votii, their behaviour on starvation and the role and regulation of threonine dehydratase

Bentley, Carolyn; Dawes, E. A.

doi:10.1007/bf00446329

Cited by 13 publications

(8 citation statements)

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“…The rapid loss of fermentative activity of P. pre'votii towards purines and nucleosides essentially parallels the loss of viability of the organism during starvation ; a similar situation was noted with serine fermentation (Montague & Dawes, 1974) but we were unable to correlate death of the organism with the loss of any of the intracellular enzymes concerned with energy generation from this amino acid or threonine (Bentley & Dawes, 1974). Direct measurements have recently revealed, however, that the ability of P. pre'votii to transport serine is lost during starvation (P. Reece & E. A. Dawes, unpublished observations).…”

Section: Discussionmentioning

confidence: 51%

“…Serine and threonine serve as energy sources for P. prdvotii during starvation (Montague & Dawes, 1974 ;Bentley & Dawes, 1974) ; our results show that adenine and xanthine can also fulfil this role. The rapid fall in total adenine nucleotide content observed by Montague & in the early stages of starvation could thus be accounted for by dephosphorylation of AMP followed by fermentation of the purine moiety.…”

Section: Discussionmentioning

confidence: 63%

“…The low rate of fermentation of this compound (Table 2) could be due to poor permeation but our inability to secure active cell-free extracts precluded further investigation of the fermentation pathway. The pattern of fermentation with intact cells reveals, however, that P. pre'votii differs from the related P. aerogenes (Whiteley, 1952) in its activity towards purines and pyrimidines, as well as towards amino acids (Bentley & Dawes, 1974).…”

Section: Discussionmentioning

confidence: 94%

“…Bacterial suspensions under nitrogen released CO, and H, endogenously, in the approximate ratio of 10 : I , at a steady but low rate. Rates varied somewhat with different preparations but the total gas (CO, plus H,) evolution was usually in the range 0.05 to 0.22 pmol h-l (mg dry wt)-l. Clearly serine and threonine, the amino acids principally concerned with energy production in P. pre'votii, could not be wholly responsible since their fermentation yields equimolar amounts of these gases (Bentley & Dawes, 1974). The other principal products were ammonia, acetate and butyrate with small amounts of formate and propionate (Table I).…”

Section: R E S U L T Smentioning

confidence: 99%

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Fermentation of Purines and their Effect on the Adenylate Energy Charge and Viability of Starved Peptococcus pravotii

Reece¹,

Tóth²,

Dawes

1976

Journal of General Microbiology

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SUMMARYThe principal products of endogenous metabolism of the obligate anaerobe Peptococcus pre'votii were carbon dioxide, ammonia, acetate and butyrate, which are also produced by fermentation of nucleosides, purines and ribose, thus supporting our previous finding that RNA is the only cellular component to undergo substantial degradation under starvation conditions. Minor products were hydrogen, formate and propionate. The stoicheiometries of fermentation of xanthine, adenine, adenosine and ribose were determined. The ability to ferment exogenous nucleosides, purines and ribose declined rapidly on starvation, as did the ability to generate ATP from xanthine, and was paralleled by the loss of viability. However, addition of pulses of xanthine or adenine to organisms in the early stages of starvation sustained their adenylate energy charge and prolonged their viability. Evidence suggests that P. prhotii dies when its principal energy source (RNA) is depleted, and a possible explanation is that the transport function of the cytoplasmic membrane decays rapidly under these conditions so that, on transfer to a recovery medium, uptake of nutrients is inadequate to support growth.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 94%

Section: R E S U L T Smentioning

confidence: 99%

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Fermentation of Purines and their Effect on the Adenylate Energy Charge and Viability of Starved Peptococcus pravotii

Reece¹,

Tóth²,

Dawes

1976

Journal of General Microbiology

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“…Studies performed with species of several other genera (viz., the genera Peptostreptococcus [4, 421, Campylobacter [22, 311, Acidaminobacter [39], Megasphaera [41], Eubacterium [12, 451, and SeZenomonas [15,32]), most of which are members of the low-G+C-content Clostridium subphylum of the gram-positive bacteria, have also contributed to our knowledge of anaerobic metabolism of amino acids.…”

mentioning

confidence: 99%

Clostridium acetireducens sp. nov., a Novel Amino Acid-Oxidizing, Acetate-Reducing Anaerobic Bacterium

Örlygsson

Krooneman

Collins

et al. 1996

International Journal of Systematic Bacteriology

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Strain 30AT (T = type strain), which was isolated from an anaerobic bioreactor fed on waste from a potato starch factory in De Krim, The Netherlands, is a nonmotile, gram-positive, anaerobic, rod-shaped organism that is able to degrade various amino acids, including alanine, leucine, isoleucine, valine, serine, and threonine. Acetate is required as an electron acceptor for the utilization of alanine, valine, leucine, and isoleucine. Other growth substrates, including pyruvate, a-ketobutyrate, a-ketoisocaproate, cu-keto-3-methylvalerate, a-ketoisovalerate, and peptone, are intermediates in amino acid catabolism. Strain 30AT utilizes neither the branched-chain amino acids nor alanine via interspecies hydrogen transfer with methanogenic and sulfatereducing bacteria or via the Stickland reaction with proline or glycine as an electron acceptor. No growth occurs with the following electron acceptors: fumarate, nitrate, nitrite, sulfite, sulfate, and oxygen. Yeast extract is required for growth. Sugars are not degraded. The optimal temperature and optimal pH for growth are 39 to 43°C and 6.4 to 7.6, respectively. The results of a 16s rRNA sequence analysis phylogenetically placed strain 30AT in Clostridium group I (genus Clostridium sensu stricto), where it forms a new and distinct line of descent.In most investigations of anaerobic degradation of amino acids the workers have focused on fermentation by Clostridium species (1,3,9,10,17,27,28). Studies performed with species of several other genera (viz., the genera Peptostreptococcus [4, 421, Campylobacter [22, 311, Acidaminobacter [39], Megasphaera [41], Eubacterium [12, 451, and SeZenomonas [15,32]), most of which are members of the low-G+C-content Clostridium subphylum of the gram-positive bacteria, have also contributed to our knowledge of anaerobic metabolism of amino acids.Several workers have reported that in addition to the usual end products produced during fermentation of amino acids (i.e., carbon dioxide, volatile fatty acids, and ammonia), hydrogen is formed (3,4,15,39,(42)(43)(44). The importance of hydrogen consumption in interspecies hydrogen transfer during the degradation of amino acids has been investigated during the last decade (30,33,34,35,39,45). The first step in the degradation of several amino acids (e.g., the branched-chain amino acids) is invariably an oxidative deamination that is usually followed by an oxidative decarboxylation of the resulting keto acid (1). Oxidative deaminations are always endergonic under standard conditions and therefore depend on the removal of the electrons produced in this step (35). It is known that these reactions are also possible through the Stickland reaction (43,44) in addition to interspecies hydrogen transfer. In previous studies of the importance of interspecies hydrogen transfer for amino acid degradation in anaerobic mixed cultures, researchers obtained preliminary evidence that there are fermentative organisms that use acetate as an electron sink (33). In this paper we describe the properties of strain 3...

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Utilization of Serine, Leucine, Isoleucine, and Valine byThermoanaerobacter brockiiin the Presence of Thiosulfate orMethanobacteriumsp. as Electron Acceptors

Fardeau¹,

Patel

Magot

et al. 1997

Anaerobe

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The energy-yielding reactions of Peptococcus pr�votii, their behaviour on starvation and the role and regulation of threonine dehydratase

Cited by 13 publications

References 38 publications

Fermentation of Purines and their Effect on the Adenylate Energy Charge and Viability of Starved Peptococcus pravotii

Fermentation of Purines and their Effect on the Adenylate Energy Charge and Viability of Starved Peptococcus pravotii

Clostridium acetireducens sp. nov., a Novel Amino Acid-Oxidizing, Acetate-Reducing Anaerobic Bacterium

Utilization of Serine, Leucine, Isoleucine, and Valine byThermoanaerobacter brockiiin the Presence of Thiosulfate orMethanobacteriumsp. as Electron Acceptors

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