Utilization of Serine, Leucine, Isoleucine, and Valine byThermoanaerobacter brockiiin the Presence of Thiosulfate orMethanobacteriumsp. as Electron Acceptors

Fardeau, Marie‐Laure; Patel, Bharat K. C.; Magot, Michel; Ollivier, Bernard

doi:10.1006/anae.1997.0126

Cited by 47 publications

(47 citation statements)

References 43 publications

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“…Alanine, glutamate, isoleucine, leucine, methionine and valine were only utilized in the presence of thiosulfate as electron acceptor. The same results were obtained for Thermoanaerobacter species (Faudon et al, 1995 ;Fardeau et al, 1997). This dependence on thiosulfate can be explained by the metabolic pathways used for degradation.…”

Section: Discussionsupporting

confidence: 76%

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

Surkov

Дубинина²,

Lysenko³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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The pH limits for growth were 5 5 to 8 0, with optimal growth at pH 6 5-7 0. All isolates were obligately anaerobic and slightly halophilic and grew in media containing 0 5-5 % NaCl with an optimum at 2 % NaCl. All strains were chemoorganoheterotrophic, having a fermentative type of metabolism and utilized proteins, peptides, amino acids and some organic acids, but not sugars, fatty acids or alcohols. Some organic substrates (isoleucine, valine, alanine, glutamate) were utilized only by strain SR12 T in the presence of sulfur or thiosulfate. Fermentation of citrate yielded mainly acetate, CO 2 and H 2 . Sulfur and thiosulfate were reduced to hydrogen sulfide during the fermentation of organic substances, which increased cell yields and growth rates. Sulfate, sulfite, fumarate, nitrate, Fe 2 O 3 , MnO 2 , DMSO and elemental selenium were not used as electron acceptors by these strains. The GMC contents of the DNA were 51 mol % for strains SR12 T , SR13 and SR15 T and 52 mol % for strain WS100 T . Based on morphological, physiological and phylogenetic similarities, all four isolates could be assigned to three new species of the genus Dethiosulfovibrio, named Dethiosulfovibrio russensis (type strain DSM 12538 T ), Dethiosulfovibrio marinus (type strain DSM 12537 T ) and Dethiosulfovibrio acidaminovorans (type strain DSM 12590 T ).

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Section: Discussionsupporting

confidence: 76%

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

Surkov

Дубинина²,

Lysenko³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…For degrading the proteolysis-derived monomers, this organism expresses pathways for catabolizing amino acids that are strictly syntrophic (that is, Ile/Leu/Val/Pro) and also fermentable (that is, Gly/Ser/Thr/Glu/Asp/ Asn) (Supplementary Table S10; Schink and Stams, 2013). More specifically, as observed in other syntrophs (Wildenauer and Winter, 1986;Fardeau et al, 1997;Baena et al, 1998;Plugge et al, 2002), Ile/Leu/Val and Pro are catabolized to branchedchain fatty acids and propionate, respectively, along with H 2 and CO 2 (Supplementary Figure S4 and Supplementary Table S10). To support H 2 -generating electron disposal, Marinimicrobia expresses Hdr-Ifo, Rnf and ECHyd ( Figure 2).…”

Section: Hdrabc (Supplementarymentioning

confidence: 97%

Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor

et al. 2015

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Ecogenomic investigation of a methanogenic bioreactor degrading terephthalate (TA) allowed elucidation of complex synergistic networks of uncultivated microorganisms, including those from candidate phyla with no cultivated representatives. Our previous metagenomic investigation proposed that Pelotomaculum and methanogens may interact with uncultivated organisms to degrade TA; however, many members of the community remained unaddressed because of past technological limitations. In further pursuit, this study employed state-of-the-art omics tools to generate draft genomes and transcriptomes for uncultivated organisms spanning 15 phyla and reports the first genomic insight into candidate phyla Atribacteria, Hydrogenedentes and Marinimicrobia in methanogenic environments. Metabolic reconstruction revealed that these organisms perform fermentative, syntrophic and acetogenic catabolism facilitated by energy conservation revolving around H 2 metabolism. Several of these organisms could degrade TA catabolism by-products (acetate, butyrate and H 2 ) and syntrophically support Pelotomaculum. Other taxa could scavenge anabolic products (protein and lipids) presumably derived from detrital biomass produced by the TA-degrading community. The protein scavengers expressed complementary metabolic pathways indicating syntrophic and fermentative step-wise protein degradation through amino acids, branched-chain fatty acids and propionate. Thus, the uncultivated organisms may interact to form an intricate syntrophy-supported food web with Pelotomaculum and methanogens to metabolize catabolic by-products and detritus, whereby facilitating holistic TA mineralization to CO 2 and CH 4 .

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“…Many syntrophic bacteria isolated previously have broad metabolic capacities; some of them are capable of conserving energy by coupling the oxidation of organics with sulfate respiration (11,25), while others can perform sugar fermentation (8,17), iron reduction (13), and/or homoacetogenesis (16,27). In contrast, P. thermopropionicum has a limited spectrum of energy metabolism that is mostly related to syntrophy (30,31).…”

Section: Pelotomaculum Thermopropionicum: An Expert Of Syntrophymentioning

confidence: 99%

Ecological and Evolutionary Interactions in Syntrophic Methanogenic Consortia

Kato

Watanabe

2010

Microb. Environ.

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In natural and engineered ecosystems, diverse species of microbes coexist and interact, resulting in the emergence of community functions. Since microbes have evolved under such circumstances, it is reasonable to deduce that they have acquired strategies for specific interspecies interactions in complex microbial communities. In this review, we discuss the ecological and evolutionary interactions in syntrophic methanogenic consortia comprised of organic acid-oxidizing bacteria and methanogenic archaea. These microbes are known to exhibit mutual interactions (syntrophy), although the molecular mechanisms underlying these sophisticated partnerships have only just been discovered. In addition, recent genomic studies have provided insights into evolutionary interactions among members of methanogenic consortia, from which a novel concept termed "niche-associated evolution" has been proposed for interpreting how specialists evolve in a biological community. We suggest that microbial interspecies interactions are much more complex and sophisticated than hitherto realized and pivotal to the development and functioning of microbial communities.

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Utilization of Serine, Leucine, Isoleucine, and Valine byThermoanaerobacter brockiiin the Presence of Thiosulfate orMethanobacteriumsp. as Electron Acceptors

Cited by 47 publications

References 43 publications

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments.

Microbial dark matter ecogenomics reveals complex synergistic networks in a methanogenic bioreactor

Ecological and Evolutionary Interactions in Syntrophic Methanogenic Consortia

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