The enamel knot-like structure is eternally maintained in the apical bud of postnatal mouse incisors

Nakatomi, Chihiro; Nakatomi, Mitsushiro; Saito, Kan; Harada, Hidemitsu; Ohshima, Hayato

doi:10.1016/j.archoralbio.2015.05.001

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…However, unlike the nearby oral epithelium, the dental epithelium expresses Shh [12][13][14][15][16]. During the period of tooth crown formation, Shh-expressing cells are strictly localized in the dental epithelium, including the enamel knot that corresponds to future cusps, as well as ameloblast-lineage cells [17][18][19]. On the other hand, Ptch-positive cells and its downstream proteins are located in the dental mesenchyme in the absence of Shh expression [20].…”

Section: Introductionmentioning

confidence: 99%

Sonic Hedgehog Signaling and Tooth Development

Hosoya

Shalehin

Takebe

et al. 2020

IJMS

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Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages. A number of studies have analyzed the function of Shh signaling at different stages of tooth development and have revealed that Shh signaling regulates the formation of various tooth components, including enamel, dentin, cementum, and other soft tissues. In addition, dental mesenchymal cells positive for Gli1, a downstream transcription factor of Shh signaling, have been found to have stem cell properties, including multipotency and the ability to self-renew. Indeed, Gli1-positive cells in mature teeth appear to contribute to the regeneration of dental pulp and periodontal tissues. In this review, we provide an overview of recent advances related to the role of Shh signaling in tooth development, as well as the contribution of this pathway to tooth homeostasis and regeneration.

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Section: Introductionmentioning

confidence: 99%

Sonic Hedgehog Signaling and Tooth Development

Hosoya

Shalehin

Takebe

et al. 2020

IJMS

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Quiescent adult stem cells in murine teeth are regulated by Shh signaling

et al. 2017

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The mechanisms regulating the maintenance of quiescent adult stem cells in teeth remain to be fully elucidated. Our aim is to clarify the relationship between BrdU label-retaining cells (LRCs) and sonic hedgehog (Shh) signaling in murine teeth. After prenatal BrdU labeling, mouse pups were analyzed during postnatal day 1 (P1) to week 5 (P5W). Paraffin sections were processed for immunohistochemistry for BrdU, Sox2, Gli1, Shh, Patched1 (Ptch1) and Ki67 and for in situ hybridization for Shh and Ptch1. Dense LRCs, Gli1-(+) cells and Ptch1-(+) cells were co-localized in the outer enamel epithelium of the apical bud and apical dental papilla of incisors. In developing molars, dense LRCs were numerous at P1 but then decreased in number over the course of odontogenesis and were maintained in the center of pulp tissue. Gli1-(+) cells were maintained in the pulp horn during the examined stages, while they increased in number and were maintained in the center of pulp tissue during P2-5W. Ptch1-(+) cells were localized in the pulp horn at P1 and increased in number in the center of the pulp after P3W. Shh mRNA was first expressed in the enamel epithelium and then shifted to odontoblasts and other pulp cells. Shh protein was distributed in the epithelial and mesenchymal tissues of incisors and molars. These findings suggest that quiescent dental stem cells are regulated by Shh signaling, and that Shh signaling plays a crucial role in the differentiation and integrity of odontoblasts during epithelial-mesenchymal interactions and dentinogenesis.

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