The emerging role of miR‐128 in musculoskeletal diseases

Shang, Qi; Shen, Gengyang; Chen, Guifeng; Zhang, Zhida; Yu, Xiang; Zhao, Wenhua; Zhang, Peng; Chen, Honglin; Tang, Kai Dun; Yu, Fuyong; Tang, Jingjing; Liang, De; Jiang, Xiaobing

doi:10.1002/jcp.30179

Cited by 18 publications

(15 citation statements)

References 142 publications

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“…MiR-370-3p has been shown to down-regulate ACADSB gene expression and inhibit the formation of slow-twitch muscle fiber [ 40 ]. Additionally, some of the DEmiRs identified in our study, such as eca-miR-192, eca-miR-10a and eca-miR-128, are involved in muscle cell proliferation, differentiation and energy expenditure [ 41 , 42 , 43 ]. These results suggested that some DEmiRs identified in our study might also be involved in skeletal muscle fiber development and composition in donkeys.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

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The skeletal muscle fiber profile is closely related to livestock meat quality. However, the molecular mechanisms determining muscle fiber types in donkeys are not completely understood. In this study, we selected the psoas major muscle (PM; mainly composed of oxidative-type muscle fibers) and biceps femoris muscle (BF; mainly composed of glycolytic-type muscle fibers) and systematically compared their mRNA and microRNA transcriptomes via RNA-seq. We identified a total of 2881 differentially expressed genes (DEGs) and 21 known differentially expressed miRNAs (DEmiRs). Furthermore, functional enrichment analysis showed that the DEGs were mainly involved in energy metabolism and actin cytoskeleton regulation. The glycolysis/gluconeogenesis pathway (including up-regulated genes such as PKM, LDHA, PGK1 and ALDOA) was more highly enriched in BF, whereas the oxidative phosphorylation pathway and cardiac muscle contraction (including down-regulated genes such as LDHB, ATP2A2, myosin-7 (MYH7), TNNC1, TPM3 and TNNI1) was more enriched in PM. Additionally, we identified several candidate miRNA–mRNA pairs that might regulate muscle fiber types using the integrated miRNA–mRNA analysis. Combined with the results of protein–protein interaction (PPI) analysis, some interesting DEGs (including ACTN3, TNNT3, TPM2, TNNC2, PKM, TNNC1 and TNNI1) might be potential candidate target genes involved in the miRNA-mediated regulation of the myofibril composition. This study is the first to indicate that DEmiRs, especially eca-miR-193a-5p and eca-miR-370, and potential candidate target genes that are mainly involved in actin binding (e.g., ACTN3, TNNT3 and TNNC1) and the glycolysis/gluconeogenesis pathways (e.g., PKM) might coregulate the myofibril composition in donkeys. This study may provide useful information for improving meat quality traits in Dezhou donkeys.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Shi

et al. 2022

Genes

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“…MicroRNAs (miRNAs) consist of 19–25 nucleotides and regulate gene expressions at the posttranscriptional level by binding to 3′UTRs of target mRNAs ( 12 ). Accumulating evidence points to an intimate connection between miRNAs and bone metabolism, keeping balanced by osteoblastic bone formation and osteoclastic bone resorption ( 12 , 13 ). However, the studies about the action mechanisms of miRNAs on GIOP are still limited.…”

Section: Introductionmentioning

confidence: 99%

Zuo-Gui-Wan Aqueous Extract Ameliorates Glucocorticoid-Induced Spinal Osteoporosis of Rats by Regulating let-7f and Autophagy

et al. 2022

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ObjectiveThis study proposes to explore the protective effect of Zuo-Gui-Wan (ZGW) aqueous extract on spinal glucocorticoid-induced osteoporosis (GIOP) in vivo and in vitro, and the underlying mechanisms of ZGW in GIOP and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) were conducted.MethodsIn vivo, SD rats were randomly divided into three groups: control group (CON), dexamethasone (DEXM) group, and ZGW group, which were given vehicle, DEXM injection, and ZGW intragastric administration at the same time. Vertebral bone microarchitecture, biomechanics, histomorphology, serum AKP activity, and the autophagosome of osteoblasts were examined. The mRNA expressions of let-7f, autophagy-associated genes (mTORC1, Beclin-1, ATG12, ATG5, and LC3), Runx2, and CTSK were examined. In vitro, the let-7f overexpression/silencing vector was constructed and transfected to evaluate the osteogenic differentiation of BMSCs. Western blot was employed to detect the expression of autophagy-associated proteins (ULK2, ATG5, ATG12, Beclin-1, LC3).ResultsIn vivo, ZGW promoted the bone quantity, quality, and strength; alleviated histological damage; increased the serum AKP activity; and reduced the autophagosome number in osteoblasts. Moreover, ZGW increased the let-7f, mTORC1, and Runx2 mRNA expressions and reduced the Beclin-1, ATG12, ATG5, LC3, and CTSK mRNA expressions. In vitro, bioinformatics prediction and dual luciferase reporter gene assay verified that let-7f targeted the binding to ULK2 and negatively regulated the ULK2 expression. Furthermore, by let-7f overexpression/silencing, ZGW may promote osteoblast differentiation of BMSCs by regulating let-7f and autophagy as evidenced by Western blot (ULK2, ATG5, ATG12, Beclin-1, LC3).ConclusionsZGW may ameliorate GC-induced spinal osteoporosis by promoting osteoblast differentiation of BMSCs by activation of let-7f and suppression of autophagy.

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“…The expression of miR-199b-5p, miR-10a-5p, miR-10b-5p, miR-24-1-5p, miR-223-3p, and miR-338-3p was suppressed, whereas miR-291a-5p, miR-125b-1-3p, and miR-291b-5p expression was enhanced (Table 3, Ref. [100][101][102][103][104][105][106][107][108]) (Fig. 2).…”

Section: Micrornas That Regulate Osteoclastogenesis and Osteoresorptionmentioning

confidence: 94%

“…Numerous studies have shown that miR-128 is involved in the regulation of signaling pathways associated with osteoclastogenesis [102,109] and with reactive oxygen species, inflammation, and fibrosis in many tissue types [109]. In HK2 cells, miR-128-3p was shown to inhibit apoptosis and inflammation by targeting the TGFBR2 gene [110].…”

Section: Micrornas That Regulate Osteoclastogenesis and Osteoresorptionmentioning

confidence: 99%

MicroRNA Regulation of Bone Marrow Mesenchymal Stem Cells in the Development of Osteoporosis in Obesity

Vulf¹,

Хлусов²,

Yurova³

et al. 2022

Front. Biosci. (Schol Ed)

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Obesity and osteoporosis are global health problems characterized by high rates of prevalence and mortality due to complications. As people with visceral obesity age, the adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) increases, and adipocytes become the predominant stromal cells in the bone marrow microenvironment, which hinders the physiological regeneration and mineralization of bone tissue. Primary and secondary osteoporosis remain severe progressive diseases. Both osteoporosis and obesity are associated with microRNAs (miRNAs) that induce adipogenesis and osteoresorption. This review presents analyses of the roles and clinical potential of miRNAs in the epigenetic control of BMSC differentiation and the formation and function of osteoclasts in osteoporosis with and without obesity. Understanding the fine-tuned regulation of the expression of genes critical for the balance of osteogenesis/osteolysis processes may provide hope for the development of effective and safe osteoporosis therapies in the future.

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The emerging role of miR‐128 in musculoskeletal diseases

Cited by 18 publications

References 142 publications

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys

Zuo-Gui-Wan Aqueous Extract Ameliorates Glucocorticoid-Induced Spinal Osteoporosis of Rats by Regulating let-7f and Autophagy

MicroRNA Regulation of Bone Marrow Mesenchymal Stem Cells in the Development of Osteoporosis in Obesity

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