The emerging role of ICP-MS in proteomic analysis

Time-resolved ICP-MS Measurement: a New Method for Elemental and Multiparametric Analysis of Single Cells

“…Not only metal-containing biomolecules, but also other biomolecules, such as amino acids, peptides, proteins, nucleic acids and microbial cells, can be successfully quantified by measuring heteroatoms, such as phosphorus and sulfur, and/or by supporting elemental tags, such as metal-labeled antigens and nanoparticles. [4][5][6][7][8][9][10] In recent years, cytometric analysis of single cells with a time-resolved ICP-MS measurement is receiving much attention for elemental [14][15][16][17][18] and multiparametric [19][20][21][22][23] analyses of single cells. Due to cellular heterogeneity within an isogenic cell population, individual analysis of cells will lead to a more sensitive representation of cell-to-cell variations, instead of a stochastic average analysis by bulk measurements.…”

Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) for Quantitative Analysis in Environmental and Life Sciences: A Review of Challenges, Solutions, and Trends

“…As a result the concept of hetero (element) tagged proteomics has been proposed, in which analytical information is generated by the complementary application of elemental and molecule-specific mass spectrometry, as well as the utilization of hetero atoms such as phosphorus, sulfur, and selenium for screening and quantification purposes. 13,[21][22][23] This progress was realized due to a number of instrumental developments during the last decade, which will be highlighted in the following sections. Their availability finally induced a paradigm shift, since it became possible to analyze elements that are highly susceptible to interference, which finally allows the number of elements that are easily detected by ICP-MS to be extended to covalently bound hetero atoms such as phosphorus, sulfur, selenium, or even halogens, which show a widespread distribution in all classes of chemical substances.…”

“…22,24,36 However, in parallel this underlines again that a successful application of such approaches strongly depends on the complementary application of moleculespecific detection techniques, such as matrix-assisted laser desorption/ionization (MALDI) or electrospray ionization mass spectrometry (ESI-MS), to gain the necessary information related to the tag stoichiometry. 21,24 The following sections of this focal point review will provide an overview and critically discuss the recent progress made in using ICP-MS hyphenated to different separation techniques for quantitative analysis in environmental and life science, via the utilization of specific element tags. In addition, trends and the current state of the art of suitable analytical techniques will be highlighted.…”

Quantification of Proteins by Measuring the Sulfur Content of Their Constituent Peptides by Means of Nano HPLC-ICPMS

“…In other words, it has become easier to identify proteins via the determination of peptide mass or peptide sequence by using electrospray ionization mass spectrometry (ESI-MS) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). 1 However, the answers to many biological questions require not only identification, but also quantification of the proteins in a cell or tissue; therefore, the quantification of proteins remains a major challenge in modern proteomics.…”