The electrophoretic mobility of normal and leukaemic cells of mice

Cook, G. M. W.; Jacobson, W.

doi:10.1042/bj1070549

Cited by 59 publications

(24 citation statements)

References 18 publications

(10 reference statements)

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“…Similar increases in basic material have also been reported on lymph node cells derived from acute lymphoblastic leukaemia (Cook and Jacobson, 1968). Sherbet and Lakshmi (1974) demonstrated that the highly malignant astrocytomas possessed additional surface material which was basic in nature.…”

Section: Discussionsupporting

confidence: 59%

Differences in the Surface Components of Normal and SV-40 Transformed 3T3 Mouse Fibroblasts

Sherbet¹,

Lakshmi²

1979

Pathobiology

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The differences in the surface components of 3T3 mouse fibroblasts and those transformed by Simian virus 40 have been examined using the isoelectric equilibrium method. The net negative surface charge density of the transformed cells appears to be marginally lower than that of the untransformed cell. Chemical modification studies of the surface groups have indicated that about 30% more cationic groups are present on the transformed cells, suggesting the occurrence of some additional basic material not detectable on the surface of normal cells. These basic groups show a modulation of ionisation characteristics in the presence of carboxylic groups. This may indicate a distribution of the cationic extra material in the vicinity of acidic glycoprotein components of the surface. This investigation has also revealed the occurrence of unidentified anionic groups which ionise at high pH, which appear to be thiol groups.

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Section: Discussionsupporting

confidence: 59%

Differences in the Surface Components of Normal and SV-40 Transformed 3T3 Mouse Fibroblasts

Sherbet¹,

Lakshmi²

1979

Pathobiology

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“…4 Results and discussion Figure 3 shows the experimental results of Zeta potential measurements at pH 7.4 for MCF7 cancer breast epithelial cells alone, MCF10A normal breast epithelial cells alone, iron oxide nanoparticles alone, CPMV nanoparticles alone as well as two types of cells after incubated with two different nanoparticles respectively for different time lengths of 30 min, 4 and 24 h. For MCF10A normal cells alone, the Zeta potential was −31.16±1.12 mV, which was much more negative compared to that of −20.32±2.43 mV for MCF7 cancer cells. This significant difference in surface electrokinetic properties between normal and transformed cancer cells has been reported before (Cook and Jacobson 1968). For iron oxide nanoparticles alone, the Zeta potential was −40.91± 0.14 mV indicating good dispersion stability.…”

Section: Theory and Analysismentioning

confidence: 52%

Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells

Zhang

Yang

Portney

et al. 2007

Biomed Microdevices

375

215

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We demonstrate the use of surface Zeta potential measurements as a new tool to investigate the interactions of iron oxide nanoparticles and cowpea mosaic virus (CPMV) nanoparticles with human normal breast epithelial cells (MCF10A) and cancer breast epithelial cells (MCF7) respectively. A substantial understanding in the interaction of nanoparticles with normal and cancer cells in vitro will enable the capabilities of improving diagnostic and treatment methods in cancer research, such as imaging and targeted drug delivery. A theoretical Zeta potential model is first established to show the effects of binding process and internalization process during the nanoparticle uptake by cells and the possible trends of Zeta potential change is predicted for different cell endocytosis capacities. The corresponding changes of total surface charge of cells in the form of Zeta potential measurements were then reported after incubated respectively with iron oxide nanoparticles and CPMV nanoparticles. As observed, after MCF7 and MCF10A cells were incubated respectively with two types of nanoparticles, the significant differences in their surface charge change indicate the potential role of Zeta potential as a valuable biological signature in studying the cellular Biomed Microdevices

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“…A few workers have already reported [Cook and J acobson, 1968;Latner andT urner. 1974: Sherbet, 1977] that the number of cationic groups on the surface of malignant cells is larger than the number present on their so-called normal counterparts.…”

Section: Discussionmentioning

confidence: 97%

Increased Trypsin Sensitivity of Cell Surface Macromolecules after Malignant Transformation

Latner¹,

Turner²

1978

Pathobiology

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The effect of treatment with 0.04% (w/v) trypsin (EC 3.4.4.4) for 3 h on the electrophoretic mobility (EPM) of polyoma-virus malignantly transformed BHK21 cells (Py6) and their normal counterparts has been investigated. These particular conditions were chosen because an earlier study had shown that such treatment released material from the Py6 cells which was not obtained from the BHK21 cells. The negative EPM of the Py6 cells at pH 7.5 was greatly increased by this treatment; whereas the EPM of the BHK21 cells remained unchanged. Active enzyme was required to produce the change. No evidence was obtained for cytolysis, cytotoxicity or uptake of the enzyme by the treated Py6 cells. Measurement of the EPM of the Py6 cells at different pH levels before and after trypsin treatment suggested that the enzyme was removing cationic groupings from the cell surface.

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The electrophoretic mobility of normal and leukaemic cells of mice

Cited by 59 publications

References 18 publications

Differences in the Surface Components of Normal and SV-40 Transformed 3T3 Mouse Fibroblasts

Differences in the Surface Components of Normal and SV-40 Transformed 3T3 Mouse Fibroblasts

Zeta potential: a surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells

Increased Trypsin Sensitivity of Cell Surface Macromolecules after Malignant Transformation

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