The electrical and chemical components of the protonmotive force in chloroplasts as measured with capillary and pH-sensitive microelectrodes

Remis, D.; Булычев, А. А.; Kurella, G. A.

doi:10.1016/0005-2728(86)90057-5

Cited by 22 publications

(9 citation statements)

References 11 publications

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“…Tip-sensitive antimony pH microelectrodes were prepared as previously described (Remis et al, 1986). First, melted antimony was sucked into Pyrex glass capillaries (1.2 mm outer diameter).…”

Section: Experimental Set-upmentioning

confidence: 99%

Light-triggered pH Banding Profile in Chara Cells Revealed with a Scanning pH Microprobe and its Relation to Self-Organization Phenomena

Булычев

Полежаев

Zykov

et al. 2001

Journal of Theoretical Biology

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Section: Experimental Set-upmentioning

confidence: 99%

Light-triggered pH Banding Profile in Chara Cells Revealed with a Scanning pH Microprobe and its Relation to Self-Organization Phenomena

Булычев

Полежаев

Zykov

et al. 2001

Journal of Theoretical Biology

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“…Also important is the role of Mg 2+ in membrane-transport processes in chloroplasts, where free Mg contributes to the regulation of photosynthetic enzyme activity (Shaul 2002). Additionally, proton pumping from the stroma into thylakoids lumen results in significant (DpH 2-3; Remis et al 1986) acidification of the thylakoid lumen. This massive light-driven transport of H + into the thylakoid lumen is electrically balanced by the counterflow of other ions (Hinnah and Wagner 1998) and, particularly, Mg 2+ (Pottosin and Scho¨nknecht 1996).…”

Section: Introductionmentioning

confidence: 99%

Effects of magnesium availability on the activity of plasma membrane ion transporters and light-induced responses from broad bean leaf mesophyll

Shabala

Hariadi

2005

Planta

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Considering the physiological significance of Mg homeostasis in plants, surprisingly little is known about the molecular and ionic mechanisms mediating Mg transport across the plasma membrane and the impact of Mg availability on transport processes at the plasmalemma. In this study, a non-invasive ion-selective microelectrode technique (MIFE) was used to characterize the effects of Mg availability on the activity of plasma membrane H+, K+, Ca2+, and Mg2+ transporters in the mesophyll cells of broad bean (Vicia faba L.) plants. Based on the stoichiometry of ion-flux changes and results of pharmacological experiments, we suggest that at least two mechanisms are involved in Mg2+ uptake across the plasma membrane of bean mesophyll cells. One of them is a non-selective cation channel, also permeable to K+ and Ca2+. The other mechanism, operating at concentrations below 30 microM, was speculated to be an H+/Mg+ exchanger. Experiments performed on leaves grown at different levels of Mg availability (from deficient to excessive) showed that Mg availability has a significant impact on the activity of plasma-membrane transporters for Ca2+, K+, and H+. We discuss the physiological significance of Mg-induced changes in leaf electrophysiological responses to light and the ionic mechanisms underlying this process.

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“…Changes in pH near the seed embryo during imbibition were measured using a glass-insulated antimony microelectrode with a tip diameter of 10-20 µm; seeds were placed into the same solution where imbibition proceeded. The microelectrode was positioned using a KM-2 micromanipulator; the position was controlled under a microscope (21). The tip of the microelectrode was placed at a distance of 5-10 µm from the embryo surface, which allowed detection of local changes that appeared earlier than did changes in the bulk solution.…”

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confidence: 99%

Effects of Elf-Emf Treatment on Wheat Seeds at Different Stages of Germination and Possible Mechanisms of Their Origin

Aksyonov¹,

Булычев²,

Grunina³

et al. 2001

Electro- and Magnetobiology

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The efficiency of weak ELF (extremely low frequency) EMF on living systems can be explained by taking into account the nonstationary processes that arise when ions pass part of the intermembrane distance during the EMF period. The periodic movement of ions in the heterogeneous medium would result in the nonlinear effects influencing the ionic strength and pH near the membrane and the release of some peripheral proteins to the water phase. Based on this notion, we studied the effects of EMF treatment (30 or 50 Hz, 30 mT) at different stages of imbibition of wheat seeds. The treatment at the stage of activation of esterases increased the leakage of the products of esterase reaction with its following retardation, which contrasts with the linear kinetics for untreated seeds and for seeds treated at earlier stages. The treatment also led to a reliable increase in pH near the embryo surface. When the wheat seeds with germinability of 50% were treated at the stage of root formation, a significant increase in the number of seeds with roots was observed. The sprout length reliably increased after this treatment with respect to seeds treated later and untreated seeds. In the latter case, only the number of seeds with sprouts increased. Long treatment of seeds during the second day of imbibition reduced the length of sprouts. The observed effects are discussed on the basis of this proposed mechanism.

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The electrical and chemical components of the protonmotive force in chloroplasts as measured with capillary and pH-sensitive microelectrodes

Cited by 22 publications

References 11 publications

Light-triggered pH Banding Profile in Chara Cells Revealed with a Scanning pH Microprobe and its Relation to Self-Organization Phenomena

Light-triggered pH Banding Profile in Chara Cells Revealed with a Scanning pH Microprobe and its Relation to Self-Organization Phenomena

Effects of magnesium availability on the activity of plasma membrane ion transporters and light-induced responses from broad bean leaf mesophyll

Effects of Elf-Emf Treatment on Wheat Seeds at Different Stages of Germination and Possible Mechanisms of Their Origin

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