The efficiency of pomegranate (Punica granatum) peel ethanolic extract in attenuating the vancomycin-triggered liver and kidney tissues injury in rats

Bohi, Khlood M. El; Abdel-Motal, Sabry M.; Khalil, Samah R.; Abd-Elaal, Mayar Mahmoud; Metwally, Mohamed M.M.; Elhady, Walaa M.

doi:10.1007/s11356-020-10999-3

Cited by 17 publications

(11 citation statements)

References 84 publications

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“…As observed in the present study, P. granatum has a considerable free radical scavenging ability; thus, it seems that the protective potential of pomegranates may depend on the alleviation of oxidative stress damage. This effect has been affirmed by the modulation of serum liver biomarkers and structural organization, decreased MDA levels, and enhanced GSH and GPx activity, especially in rats that received PAE, which agrees with the previous results [46,47]. The high potential of phenolic components to scavenge radicals might be explained by their ability to donate a hydrogen atom from their phenolic hydroxyl groups [48].…”

Section: Discussionsupporting

confidence: 88%

“…Moreover, antioxidant activity might not be the only factor responsible for the ability of pomegranates to reduce the severity of Tol hepatotoxicity; another potential therapeutic tool is linked to its anti-inflammatory and anti-apoptotic activity. It was revealed that pomegranate peel extract can reduce hepato-inflammation and oxidative stress by suppressing the production of pro-inflammatory cytokines and enhancing Nrf2 activation [47]. Thus, Nrf2 upregulation leads to an elevation in the expression of antioxidant enzymes, to combat ROS generation [51].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats

et al. 2022

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Background and Aim: Humans are more frequently exposed to chemicals in daily life by inhalation of indoor and outdoor air. However, abusers and workers are the most exposed to those chemicals and their health risks particularly, liver diseases. The present study investigated the protective effects of pomegranate juice (PJ) (Punica granatum) and pomegranate peel aqueous extract (PAE) supplementation against toluene (Tol)-induced hepatotoxicity in Wistar rats. Materials and Methods: A phytochemical analysis and assessment of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were performed for the PJ and the PAE. The in vivo study was carried out using 70 Wistar rats that were divided into seven groups, each consisting of 10 rats. All groups were treated orally for 6 weeks as follows: Control (C), positive controls (CO: 1.25 mL/kg body weight [BW]; PJ: 4 mL/kg BW; and PAE: 400 mg/kg BW), Tol (550 mg/kg BW), and a mixture each of PJ-Tol and PAE-Tol. At the end of the 45th day of study, the hepatic biochemical markers (transaminases, alkaline phosphatase, total bilirubin, albumin, and total proteins) were auto-analyzed, as well as histology and oxidative stress markers of the liver were evaluated. Results: The phytochemical analysis revealed that the DPPH scavenging activity and the total phenolic, flavonoid, and tannin contents were higher in the pomegranate peel extract versus the juice. The results also showed that Tol significantly increased liver enzyme activities and total bilirubin levels, whereas albumin and total proteins were significantly decreased. Similarly, Tol provoked a significant increase in hepatic malondialdehyde levels, with a decrease in glutathione content and glutathione peroxidase activity. The biochemical changes agreed with the hepatic histological alterations. A significant improvement in all parameters was observed in the PAE-Tol group compared with the PJ-Tol group. Conclusion: Exposure to Tol altered the hepatic antioxidant and biochemical parameters and histological profile of the rats, and PAE was more powerful than PJ in reducing Tol liver injuries through its antioxidant activity.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats

et al. 2022

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“…Such effect was demonstrated at 200 and 400 mg/kg BW, whereas at 100 mg/kg BW, incomplete nephroprotection was obtained [ 56 ]. In contrast, 100 mg/kg BW of pomegranate fruit peel extract showed effective hepatoprotective and nephroprotective properties in co-treatment with high doses of the antibiotic vancomycin, and better protection was highlighted when administrated prior to vancomycin treatment [ 57 ]. This result suggests an antagonist effect of the antibiotic and the plant extract.…”

Section: The Role Of Plants In Renal Pathophysiologymentioning

confidence: 99%

Nephroprotective Plants: A Review on the Use in Pre-Renal and Post-Renal Diseases

Tienda-Vázquez

Morreeuw

Sosa‐Hernández

et al. 2022

Plants

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Kidney diseases are expected to become the fifth leading cause of death by 2040. Several physiological failures classified as pre-, intra-, and post-renal factors induce kidney damage. Diabetes, liver pathologies, rhabdomyolysis, and intestinal microbiota have been identified as pre-renal factors, and lithiasis or blood clots in the ureters, prostate cancer, urethral obstructions, prostate elongation, and urinary tract infections are post-renal factors. Additionally, the nephrotoxicity of drugs has been highlighted as a crucial factor inducing kidney injuries. Due to the adverse effects of drugs, it is necessary to point to other alternatives to complement the treatment of these diseases, such as nephroprotective agents. Plants are a wide source of nephroprotective substances and can have beneficial effects in different levels of the physiological pathways which lead to kidney damage. In traditional medicines, plants are used as antioxidants, anti-inflammatories, diuretics, and anticancer agents, among other benefits. However, the mechanism of action of some plants empirically used remains unknown and scientific data are required to support their nephroprotective effects. The present work reviewed the plants with a beneficial effect on kidney diseases. The classification of nephroprotective plants according to the clinical definition of pre-renal, intrinsic, and post-renal factors is proposed to orient their use as complementary treatments.

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“…Studies show that oxidative stress damage plays an important role in Van-induced AKI [5,39,40] . Oxidative damage triggers the death of renal tubular cells, which accelerates acute or chronic kidney injury [41][42][43] .…”

Section: Rklotho Decreased Van-induced Oxidative Stress In Hk-2 Cellsmentioning

confidence: 99%

Klotho alleviates vancomycin-induced acute kidney injury by upregulating anti-oxidative capacity via JAK2/STAT3/GPx3 axis

Wang

Zhou

Yin

et al. 2022

Preprint

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Background and Purpose: Emerging studies indicated that Klotho is a marker of renal injury and owns a protective effect on different kinds of kidney diseases. However, the role of Klotho in vancomycin (Van)-induced acute kidney injury (AKI) is largely unclear. Hence this study aimed to explore the regulatory mechanism of Klotho in Van-AKI. Experimental Approach: The mRNA expression of Klotho and the JAK2/STAT3/GPx3 axis was assessed by RNA sequence analysis after the Van challenging; the mechanism action of Klotho was examined in vitro by Klotho small interfering RNA and recombinant Klotho. The expression of reactive oxygen species and antioxidant enzymes was detected by flow cytometry and spectrophotometry. Transmission electron microscopy was performed to scan the structural damage of mitochondria. Western blot, qPCR, and immunofluorescence were applied to further explore the function of the JAK2/STAT3/GPx3 axis in Van-AKI. Key Results: RNA sequence analysis indicated that Van challenging reduced the expression of Klotho and GPx3, but increased JAK2/STAT3. In vitro, Klotho siRNA enhanced the production of reactive oxygen species and the cell apoptosis ratio by regulating the JAK2/STAT3/GPx3 axis, while the decrease of GPx3 was prevented by specific inhibitors of JAK2/STAT3. In contrast, recombinant human Klotho showed the opposite function to Klotho siRNA. In vivo, recombinant mouse Klotho improved the anti-oxidative enzyme level and mitochondria damage as well as renal dysfunction and histological damage via upregulating anti-oxidative ability. Conclusion and Implications: To conclude, the study evidenced that recombinant Klotho ameliorated Van-induced AKI via the JAK2/STAT3/GPx3 signaling axis by upregulating anti-oxidative ability.

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The efficiency of pomegranate (Punica granatum) peel ethanolic extract in attenuating the vancomycin-triggered liver and kidney tissues injury in rats

Cited by 17 publications

References 84 publications

Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats

Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats

Nephroprotective Plants: A Review on the Use in Pre-Renal and Post-Renal Diseases

Klotho alleviates vancomycin-induced acute kidney injury by upregulating anti-oxidative capacity via JAK2/STAT3/GPx3 axis

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