Aim: This study aimed to compare the physicochemical, the biochemical, and the antioxidant characteristics of unifloral bitter honey and polyfloral sweet honey. Materials and Methods: Unifloral bitter and polyfloral sweet honey samples were, respectively, harvested in January and July, and then, they were extracted by the traditional method. The markers of refractive index, pH, free acidity, Brix index, density, reducing sugars, total polyphenols, flavonoids, tannins, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing/ antioxidant power (FRAP) were evaluated. Results: The obtained results showed that the physicochemical parameters are within the normal ranges, in which they meet the international standards (Codex Alimentarius). For biochemical constituents, matching concentrations of reducing sugars (glucose+fructose) were observed in both samples, while that of sucrose were very low in unifloral than polyfloral honey. The levels of the active ingredients showed a difference in total polyphenols and tannins of the two types of honey studied, whereas that of flavonoids were almost similar. The antioxidant activity of various samples evaluated by DPPH and FRAP indicated that unifloral has a superior activity. Conclusion: Compared to polyfloral honey, unifloral bitter honey has lower sucrose, high total polyphenols, and tannins levels, in addition to higher antioxidant potential.

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Background and Aim: Humans are more frequently exposed to chemicals in daily life by inhalation of indoor and outdoor air. However, abusers and workers are the most exposed to those chemicals and their health risks particularly, liver diseases. The present study investigated the protective effects of pomegranate juice (PJ) (Punica granatum) and pomegranate peel aqueous extract (PAE) supplementation against toluene (Tol)-induced hepatotoxicity in Wistar rats. Materials and Methods: A phytochemical analysis and assessment of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were performed for the PJ and the PAE. The in vivo study was carried out using 70 Wistar rats that were divided into seven groups, each consisting of 10 rats. All groups were treated orally for 6 weeks as follows: Control (C), positive controls (CO: 1.25 mL/kg body weight [BW]; PJ: 4 mL/kg BW; and PAE: 400 mg/kg BW), Tol (550 mg/kg BW), and a mixture each of PJ-Tol and PAE-Tol. At the end of the 45th day of study, the hepatic biochemical markers (transaminases, alkaline phosphatase, total bilirubin, albumin, and total proteins) were auto-analyzed, as well as histology and oxidative stress markers of the liver were evaluated. Results: The phytochemical analysis revealed that the DPPH scavenging activity and the total phenolic, flavonoid, and tannin contents were higher in the pomegranate peel extract versus the juice. The results also showed that Tol significantly increased liver enzyme activities and total bilirubin levels, whereas albumin and total proteins were significantly decreased. Similarly, Tol provoked a significant increase in hepatic malondialdehyde levels, with a decrease in glutathione content and glutathione peroxidase activity. The biochemical changes agreed with the hepatic histological alterations. A significant improvement in all parameters was observed in the PAE-Tol group compared with the PJ-Tol group. Conclusion: Exposure to Tol altered the hepatic antioxidant and biochemical parameters and histological profile of the rats, and PAE was more powerful than PJ in reducing Tol liver injuries through its antioxidant activity.