The efficacy of individual‐donation and minipool testing to detect low‐level hepatitis B virus DNA in Taiwan

Yang, Menghua; Li, Lei; Hung, Ying-Shen; Hung, Cheng-Shen; Allain, Jean‐Pierre; Lin, Kuo‐Sin; Tsai, Salina

doi:10.1111/j.1537-2995.2009.02357.x

Cited by 28 publications

(29 citation statements)

References 33 publications

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“…Donations were tested for HBV DNA individually with the PROCLEIX TIGRIS/PROCLEIX ULTRIO assay (Chiron/Gen-Probe, Emeryville/San Diego, California, USA) or in minipools of six with the Roche cobas s 201/cobas TaqScreen multiplex (Roche Diagnostics, Mannheim, Germany). The reported 95% HBV DNA detection limits of the two assays were 10 and 3 IU/ml, respectively 7 11…”

Section: Methodsmentioning

confidence: 98%

See 1 more Smart Citation

Occult hepatitis B infection in blood donors from South East Asia: molecular characterisation and potential mechanisms of occurrence

Candotti

Lin²,

Belkhiri³

et al. 2012

Gut

Self Cite

128

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Section: Methodsmentioning

confidence: 98%

“…In South East Asia, where genotypes B and C are prevalent, up to 90% of the population has evidence of past exposure to HBV 5–7. The prevalence of OBI previously reported among blood donors in Asia is 1:570–1:7517 in China,4 8 1:3248 in Hong Kong,9 1:894–1:1029 in Taiwan7 10 and 1:3832 in Thailand 11…”

Section: Introductionmentioning

confidence: 94%

Occult hepatitis B infection in blood donors from South East Asia: molecular characterisation and potential mechanisms of occurrence

Candotti

Lin²,

Belkhiri³

et al. 2012

Gut

Self Cite

128

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show abstract

“…In blood banks, NAT is usually based on transcription-mediated amplification (TMA) or a multiplex polymerase chain reaction (PCR) that allows the simultaneous detection of HIV-1/2 RNA, HCV RNA, and HBV DNA on individual samples (ID-NAT) even though it is quite expensive. To limit costs, NAT screening was done with pooled samples of 6-50 blood donations that, if reactive, are further tested to identify the reactive sample [55][56][57][58][59]. Some assays have been specifically designed to provide the amplification of the viral genome and the identification of reactive samples in only one step [60,61].…”

Section: Detection Of Occult Hepatitis B Infections In Blood Donorsmentioning

confidence: 99%

Occult Hepatitis Infection in Transfusion Medicine: Screening Policy and Assessment of Current Use of Anti-HBc Testing

Esposito

Sabia

Iannone

et al. 2017

Transfus Med Hemother

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HBV still represents a global risk factor in transfusion medicine. The residual risk of HBV is not limited to pre-seroconversion window period but it extends to donors with occult HBV infection (OBI) characterized by the presence of HBV DNA in liver and by the absence of the virus surface antigen. Each country developed an appropriate blood screening policy according to local HBV prevalence, yields of infectious units per different screening methods and cost-effectiveness. We underline the need of maintaining a high level of attention for OBI carrier identification in all blood banks worldwide where the screening procedures are generally based on a combination of both serological markers and nucleic acid amplification test. In this context, markers such as hepatitis B surface antibodies and hepatitis B core antibodies (anti-HBc) might be useful, although the use of this latter is highly debated and still controversial. Our aim is to give an overview on the relevant diagnostic approaches for the routine screening for HBV focusing on the feasibility of anti-HBc testing as precautionary measure in preventing OBI transmission worldwide. In our tailored algorithm, the loss of about 1% of ‘anti-HBc only' donors, does not significantly affect the blood supply while improving recipient safety.

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“…The one HIV specimen missed by minipool NAAT of the latter was traced to an identification error during specimen preparation [27,28]. Turnaround time was reduced and delivery was increased among high risk groups in a survey which piloted delivery of NAAT results through the internet [29].…”

Section: Populationmentioning

confidence: 99%

Current Trends in the detection of Acute HIV Infection among Blood Donors: Reliability of Pooled Nucleic Acid Amplification Technology and the Need for Population Specific Algorithms: A Systematic Review

PJ¹,

P²

2015

J Antivir Antiretrovir

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We conducted a systematic review of quantitative studies that evaluated the accuracy of nucleic acid amplification testing technologies (NAAT) compared their cost effectiveness and evaluated minipool NAAT against individual donor testing NAAT. PubMed, Cochrane and Google Scholar were used to identify relevant peer-reviewed journal articles published in English language between 1999 (when NAAT was introduced) and 2013. MeSH key words included: Human immunodeficiency virus OR HIV AND nucleic acid amplification techniques OR pooled NAAT AND blood donors. Additional filters include: minipool-NAAT and individual donor testing-NAAT. After screening for duplication and relevance, 50 out of 4,181 articles were selected. Thirty six (36) studies which included 5 review article, 5 retrospective cohort studies, 20 cross sectional studies, 2 statistical modeling's, 2 national guidelines and 2 prospective cohort studies were further synthesized. Articles were further sub-divided into 8 groups based their focus area which includes: prevalence, clinical sensitivity of the assay, analytical sensitivity, test technology, testing algorithm, limit of detection and cost effectiveness.Four of six studies with pool sizes of 10 to 50 donor plasmas with standard centrifugation recorded a clinical sensitivity of 100% while the remaining two whose plasma pool size of 96 and128 had sensitivities of 92.3% and 95.3%, respectively. All four studies that focused on analytical sensitivity using different samples and controls, including cadaveric samples reported 100% analytical sensitivity using various pool sizes. We recommend minipool NAAT testing after running a third generation ELISA as highly sensitive and cost effective algorithm for low income countries.

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The efficacy of individual‐donation and minipool testing to detect low‐level hepatitis B virus DNA in Taiwan

Abstract: The higher yield rate for IDT versus MP4 demonstrates the benefit to implement a more sensitive NAT strategy in regions having significant OBI carriers such as Taiwan.

Cited by 28 publications

References 33 publications

Occult hepatitis B infection in blood donors from South East Asia: molecular characterisation and potential mechanisms of occurrence

Occult hepatitis B infection in blood donors from South East Asia: molecular characterisation and potential mechanisms of occurrence

Occult Hepatitis Infection in Transfusion Medicine: Screening Policy and Assessment of Current Use of Anti-HBc Testing

Current Trends in the detection of Acute HIV Infection among Blood Donors: Reliability of Pooled Nucleic Acid Amplification Technology and the Need for Population Specific Algorithms: A Systematic Review

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