The efficacy of erythrocytes isolated from blood stored under blood bank conditions

Vani, R.; Koshy, Abel Abraham; Koushik, Amit Kumar; Kaur, Harsimran; Kumari, K. Nalini; Agrawal, M. K.; Priyanka,; Ramya,; Khatai, Smrutishree; Gowda, Vaishnavi; Kumar, Vinay

doi:10.1016/j.transci.2012.06.014

Cited by 24 publications

(16 citation statements)

References 15 publications

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“…Age of stored red blood cells (RBCs) has been shown to enhance both immunologic derangements and harmful postoperative outcomes in patients [3–5]. Studies have compared the effects of storage in RBCs <7 d old with RBCs stored for 7–30 d, but these studies used RBCs processed with conventional blood bank protocols [6–8]. Military use of warm fresh whole blood for transfusion has been associated with increased efficacy and decreased RBC storage lesions [9].…”

Section: Introductionmentioning

confidence: 99%

Immunologic profiles of red blood cells using in vitro models of transfusion

Long

Meier

Ward

et al. 2013

Journal of Surgical Research

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Background Transfusion of packed red blood cells (RBCs) produces a myriad of immunologic derangements, from suppressive to stimulatory. Proliferation of human T cells is suppressed in vitro after exposure to processed red blood cells (PRBCs). We hypothesized that this effect would be mitigated by using fresh RBCs. We also hypothesized that this suppressive effect was a generalized effect on lymphocyte proliferation and would be observed in both CD4+ and CD8+ T-cell subpopulations as well as B cells. Materials and methods We isolated human T cells from donor peripheral blood mononuclear cells and exposed them to either blood bank PRBCs or fresh RBCs from volunteer donors and stimulated them with anti-CD3/anti-CD28. Human B cells were stimulated with lipopolysaccharide and exposed to PRBCs or fresh RBCs. We measured proliferation of B cells by thymidine incorporation assays. We also treated RBCs with citrate-phosphate-dextrose (CPD) at different time points before culture them with stimulated T cells to determine the role of this common RBC storage solution in lymphocyte proliferation. Results In vitro proliferation of CD4+ and CD8+ T cells was suppressed by blood bank RBCs. This suppression is eliminated when fresh RBCs were used. The B cells showed inhibition of proliferation when exposed to similar conditions, which appeared to be consistent over serial dilutions. Fresh RBCs exposed to CPD did not appear suppressive in the first 6 h after exposure. Conclusions T-cell and B-cell proliferation inhibition by blood banked RBCs suggests a generalized effect of RBCs on cellular proliferation. The lack of suppression by fresh RBCs further suggests that something involved in blood banking alters RBC properties such that they attain a suppressive phenotype. One such blood banking component, CPD, does not appear to affect this suppressive phenotype within the first 6 h.

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Section: Introductionmentioning

confidence: 99%

Immunologic profiles of red blood cells using in vitro models of transfusion

Long

Meier

Ward

et al. 2013

Journal of Surgical Research

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“…Lipid peroxidation continues up to the formation of MDA which can modify the proteins and together with membrane lipids, cause membrane damage. Protecting activity of antioxidant defense system may preserve MDA at low level (20)(21)(22). Studies in stored whole blood components play an important role to understand the alteration of oxidative stress during storage (23).…”

Section: Discussionmentioning

confidence: 99%

In Stored Human Blood, The Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative DNA Damage

Huyut¹,

Şekeroğlu²,

Balahoroğlu³

et al. 2016

Eastern J Med

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“…The changes in PS exposure may reflect storage related changes in intracellular ATP concentration and endogenous cell antioxidant system. It was shown that RBCs stored under blood bank conditions are capable of attenuating ROS [10] and maintaining unchanged intracellular ATP levels within 2 weeks of storage [57]. Oxidative stress, ATP deprivation and cytosolic Ca 2+ depletion induce deactivation of PMCA, facilitate Ca 2+ transport and evoke acute Ca 2+ overload in the transfused cells [58].…”

Section: Discussionmentioning

confidence: 99%

“…According to the current theory of storage lesion, the fall in ATP level weakens the cell antioxidant defence resulting in a decline in glutathione peroxidase activity [3,8] and cell damage by reactive oxygen species (ROS). The levels malondialdehyde (MDA), ascorbate (ASC), dehydroascorbic acid (DHA), interleukin-6 (IL-6) were shown to increase significantly during the storage period [9,10] suggesting a progressive oxidative stress in preserved cells. It causes disruption of the cytoskeleton, aggregation of band 3 and release of vesicles [5,6,7,11,12].…”

Section: Introductionmentioning

confidence: 99%

Natural Antioxidants Improve Red Blood Cell “Survival” in Non-Leukoreduced Blood Samples

Kucherenko

Bernhardt

2015

Cell Physiol Biochem

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Background: Blood collected in an anticoagulant can be kept refrigerated in an unmodified state within 5 - 6 weeks. Oxidative damage is considered to be a one of the major factors contributing to the development of storage lesions. Lipid and membrane proteins oxidation results in changes in cation gradients that affect the cell survival. Aim: In the present study we used the natural antioxidants and ion channels blockers (L-carnosine, spermine, phloretin and their mixtures) to prolong “survival” of red blood cells (RBCs), measured as the lack of PS exposure and cell hemolysis, in the Alsever's preservative solution upon hypothermic storage. Results: We show that the mixture of carnosine (20 mM), spermine (20 µM) and phloretin (100 µM) effectively blunted phosphatidylserine (PS) exposure, Ca²⁺ accumulation and RBCs hemolysis in non-leukoreduced low (∼2%) hematocrit samples after 36 days of storage as well as after 1 day of post-storage incubation of the stored cells in physiological saline solution. In addition, a slight but significant decrease in PS exposure was observed in non-leukoreduced high (∼20%) hematocrit samples after 36 days of storage with the mixture of substances. Conclusion: We conclude that the use of the mixture of natural antioxidants (carnosine, spermine, and phloretin) as an additive to blood preservative solution provides better RBCs storage and “survival”.

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The efficacy of erythrocytes isolated from blood stored under blood bank conditions

Cited by 24 publications

References 15 publications

Immunologic profiles of red blood cells using in vitro models of transfusion

Immunologic profiles of red blood cells using in vitro models of transfusion

In Stored Human Blood, The Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative DNA Damage

Natural Antioxidants Improve Red Blood Cell “Survival” in Non-Leukoreduced Blood Samples

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