The Efficacy and Safety of Gene Transfer into the Porcine Liver In Vivo by HVJ (Sendai Virus) Liposome

Abstract

These data suggest for the first time that the use of the HVJ-liposome vector is a safe and feasible modality for liver-directed gene transfer in pigs, and it might therefore be suitable for clinical gene therapy trials.

Adenoviral Gene Delivery to Solid Tumors by Recombinant Silk–Elastinlike Protein Polymers

“…An in vivo grade silkYelastinlike polymer namely SELP-47K with the structure: [(GVGVP) 4 GKGVP(GVGVP) 3 (GAGAGS) 4 ] 12 (head and tail residues are not shown), was provided by Protein Polymer Technologies, Inc. (San Diego, CA) as 12 wt% solutions in 3 ml syringes and stored at j80-C. The frozen SELP was thawed for 5 min at room temperature and gently mixed with adenovirus (Ad.GFP or Ad.CMV.LacZ, Qbiogene, Montreal, CA) of appropriate concentrations.…”

“…However, its clinical applications are currently limited by the ratio of efficacy to toxicity (3,4). For maximum efficiency, viral vectors must reach most tumor cells.…”

Novel Cationic Lipid That Delivers siRNA and Enhances Therapeutic Effect in Lung Cancer Cells

“…The success of gene therapy critically depends on the safety and efficacy of the transfection vector used in delivering the therapeutic gene 2,3. Small interfering RNA (siRNA) that can induce sequence-specific gene silencing has been developed as a potential cancer therapy agent 4-6.…”

“…Recently, new approaches such as immunotherapy, targeted agents and gene therapy are developed for cancer therapy. The success of gene therapy critically depends on the safety and efficacy of the transfection vector used in delivering the therapeutic gene. , Small interfering RNA (siRNA) that can induce sequence-specific gene silencing has been developed as a potential cancer therapy agent. − Cancer growth inhibition was observed after siRNA mediated knockdown of the overexpressed oncogenes such as EGFR that are essential to NSCLC proliferation . Cationic liposomes as well as viral vectors have been shown to be powerful tools to deliver siRNA …”