Adenoviral Gene Delivery to Solid Tumors by Recombinant Silk–Elastinlike Protein Polymers

Hatefi, Arash; Cappello, Joseph; Ghandehari, Hamidreza

doi:10.1007/s11095-006-9200-5

Cited by 64 publications

(76 citation statements)

References 20 publications

(21 reference statements)

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“…Further, a histological study revealed that more abundant Ad particles were present three weeks following virus administration in tumor those treated with Ad encapsulated in SELP hydrogel in comparison to tissues treated with naked Ad. These results are in a good agreement with previous studies in which intratumorally administered naked Ad was shown to rapidly disseminate into the surrounding tissue and cleared out by host immunity [109,111,112], resulting in poor viral accumulation in tumor tissues. Ad-mediated suppression of C-Met expression in tumor tissue was also significantly enhanced and prolonged by encapsulation with SELP matrix.…”

Section: Hydrogels For Local and Sustained Delivery Of Viral Vectorssupporting

confidence: 92%

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Kasala

Yoon

Hong

et al. 2016

Nanomedicine (Lond.)

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Viral vectors are promising gene carriers for cancer therapy. However, virus-mediated gene therapies have demonstrated insufficient therapeutic efficacy in clinical trials due to rapid dissemination to nontarget tissues and to the immunogenicity of viral vectors, resulting in poor retention at the disease locus and induction of adverse inflammatory responses in patients. Further, the limited tropism of viral vectors prevents efficient gene delivery to target tissues. In this regard, modification of the viral surface with nanomaterials is a promising strategy to augment vector accumulation at the target tissue, circumvent the host immune response, and avoid nonspecific interactions with the reticuloendothelial system or serum complement. In the present review, we discuss various chemical modification strategies to enhance the therapeutic efficacy of viral vectors delivered either locally or systemically. We conclude by highlighting the salient features of various nanomaterial-coated viral vectors and their prospects and directions for future research.

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Section: Hydrogels For Local and Sustained Delivery Of Viral Vectorssupporting

confidence: 92%

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Kasala

Yoon

Hong

et al. 2016

Nanomedicine (Lond.)

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“…Silk elastin-like polypeptides (SELPs) are chimeric proteins designed to retain the mechanical properties of silk, while incorporating the high solubility and environmental sensitivity of ELPs [139,161,168]. Some SELPs can be injected through hypodermic needles, and they then form insoluble hydrogels at the injection site without chemical assistance [162,[169][170][171].…”

Section: Silk-elp Hybridsmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

280

231

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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“…Moreover, lysinehistidine (KH) repeats fused to basic fibroblast growth factor (bFGF) were prepared by Hatefi et al (34) and showed enhanced transfection compared to KH repeats alone. It is of special note that to our knowledge, only the retated silk elastinlike polymers (SELP) pioneered by Ghandehari et al have been investigated for matrix-mediated naked DNA and adenoviral gene therapy (35,36). However, there are no reports of systemic gene delivery utilizing thermosensitive ELP.…”

Section: Introductionmentioning

confidence: 99%

Intelligent Biosynthetic Nanobiomaterials (IBNs) for Hyperthermic Gene Delivery

2007

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K8-ELP(1-60) was successfully designed and purified through recombinant means with efficient and stable condensation of pDNA at N/P ratios>0.25 and polyplex particle size<115 nm. MCF-7 cells successfully expressed EGFP with minimal cytotoxicity compared to positive controls; moreover, polyplexes retained sharp, thermotransitive kinetics within a narrow Tt range at clinically relevant hyperthermic temperatures, where the decrease of Tt was due to the increased hydrophobicity upon charge neutralization.

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Adenoviral Gene Delivery to Solid Tumors by Recombinant Silk–Elastinlike Protein Polymers

Abstract: These results suggest the potential of SELPs in local adenoviral delivery to solid tumors as an alternative approach to intratumoral virus infusion.

Cited by 64 publications

References 20 publications

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Evolving Lessons on Nanomaterial-Coated Viral Vectors for Local and Systemic Gene Therapy

Peptide-based biopolymers in biomedicine and biotechnology

Intelligent Biosynthetic Nanobiomaterials (IBNs) for Hyperthermic Gene Delivery

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