The effects of vitamins C and E on the growth, tissue accumulation and prophylactic response to thermal and acidic stress of hybrid catfish

Abstract

This study investigated the effects of the co-supplementation of vitamins C (0, 500, and 1000 mg kg À1 ) and E (0, 62.5, and 125 mg kg À1 ) on the growth performance, haematology and the modulation of blood stress indicators and immune parameters in hybrid catfish (Clarias macrocephalus 9 Clarias gariepinus) under combinations of thermal and acidic stress. Supplementation of vitamins C and E influenced the growth, haematological indices, serum chloride, plasma protein and immune parameters (lysozyme, total immunoglobulin and alternative complement haemolytic assay) (P < 0.05). Although vitamins C and E did not prevent a significant reduction in serum chloride, they minimized not only the modulation of blood glucose and plasma protein, but also the reduction in immune parameters (P < 0.05) owing to stress. Our results demonstrated that co-supplementation of 500 mg kg À1 vitamin C and 125 mg kg À1 vitamin E, or 1000 mg kg À1 vitamin C alone, for four weeks and co-supplementation of both vitamins at low levels (vitamins C at 500 mg kg À1 and E at 62.5 mg kg À1 ) for eight weeks had beneficial effects on the growth, amelioration of stress-mediated adverse changes in the physiological and immunosuppressive responses of hybrid catfish under stressful conditions.

Effects of inulin and Jerusalem artichoke (Helianthus tuberosus) as prebiotic ingredients in the diet of juvenile Nile tilapia (Oreochromis niloticus)

“…Total immunoglobulin was measured according to Siwicki et al (1994). Using fish serum, lysozyme activity was estimated as described by Pitaksong et al (2013), and ACH50 activity was measured according to Sunyer and Tort (1995).…”

The effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) tuber on the growth performance, haematological, blood chemical and immune parameters of Nile tilapia (Oreochromis niloticus) fingerlings

“…Total Ig was measured from the difference in total plasma protein prior to and after the precipitation of plasma immunoglobulin, employing 12% polyethylene glycol. Using fish serum, lysozyme and ACH50 activities were measured as described by Pitaksong, Kupittayanant and Boonanuntanasarn (2013). Serum lysozyme activity was analysed using a turbidimetric assay.…”

Effects of dietary vitamin E and astaxanthin on growth, skin colour and antioxidative capacity of large yellow croaker Larimichthys crocea

“…Meanwhile, vitamin E is also involved in the vitamin E, and their body vitamin E mainly depends on the dietary intake. Vitamin E deficiency may lead to darkened skin, lipid oxidation, anaemia, muscular and liver degeneration and lower growth performance (Chen et al, 2004;Montero et al, 2001;Niu, Jia et al, 2014;Pitaksong, Kupittayanant, & Boonanuntanasarn, 2013). Meanwhile, excessive doses of vitamin E might also exert a pro-oxidant effect and reduce growth performance (Li, Liang, et al, 2014).…”