The Effects of Vitamin C Supplementation on Protein Oxidation in Healthy Volunteers

Carty, Julie L.; Bevan, Ruth; Waller, Helen; Mistry, Nalini; Cooke, Marcus S.; Lunec, Joseph; Griffiths, Helen R.

doi:10.1006/bbrc.2000.3014

Cited by 123 publications

(69 citation statements)

References 21 publications

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“…Similar, albeit non-significant, increases in urinary 8-oxodG were noted during vitamin C supplementation (Priemé et al 1997) or high fruit and vegetable diet (Hertog et al 1997). This lack of significance during supplementation is consistent with the findings of Cooke et al (1998) and led to the suggestion of a post-supplementation effect of vitamin C. This effect is exemplified by a recent result with 400 mg/d supplementation for 10-20 weeks (Carty et al (2000); Fig. 2).…”

Section: Vitamin Csupporting

confidence: 78%

Role of dietary antioxidants in the prevention of in vivo oxidative DNA damage

Cooke

Evans

Mistry

et al. 2002

NRR

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Epidemiological evidence consistently shows that diets high in fresh fruit and vegetables significantly lower cancer risk. Given the postulated role of oxidative DNA damage in carcinogenesis, the assumption has been made that it is the antioxidant properties of food constituents, such as vitamin C, E and carotenoids, which confer protection. However, epidemiological studies with specific antioxidants, either singly or in combination, have not, on the whole, supported this hypothesis. In contrast, studies examining the in vitro effect of antioxidants upon oxidative DNA damage have generally been supportive, in terms of preventing damage induction. The same, however, cannot be said for the in vivo intervention studies where overall the results have been equivocal. Nevertheless, recent work has suggested that some dietary antioxidants may confer protective properties through a novel mechanism, unrelated to their conventional free-radical scavenging abilities. Upregulation of antioxidant defence, xenobiotic metabolism, or DNA-repair genes may all limit cellular damage and hence promote maintenance of cell integrity. However, until further work has clarified whether dietary supplementation with antioxidants confers a reduced risk of cancer and the mechanism by which this effect is exerted, the recommendation for a diet rich in fruit and vegetables remains valid empirically.

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Section: Vitamin Csupporting

confidence: 78%

Role of dietary antioxidants in the prevention of in vivo oxidative DNA damage

Cooke

Evans

Mistry

et al. 2002

NRR

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“…Here carbonyls were analysed to provide insight into the systemic steady state of protein oxidation following almond intervention and were determined by ELISA as previously described by Carty et al [21].…”

Section: Protein Carbonyl Determinationmentioning

confidence: 99%

An almond-enriched diet increases plasma α-tocopherol and improves vascular function but does not affect oxidative stress markers or lipid levels

Choudhury

Clark

Griffiths

2014

Free Radical Research

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Vascular dysfunction is one of the major causes of cardiovascular (CV) mortality and increases with age. Epidemiological studies suggest that Mediterranean diets and high nut consumption reduce CV disease risk and mortality while increasing plasma α-tocopherol. Therefore, we have investigated whether almond supplementation can improve oxidative stress markers and CV risk factors over 4 weeks in young and middle-aged men.Healthy middle-aged men (56+5.8years), healthy young men (22.1+2.9years) and young men with two or more CV risk factors (27.3+5years) consumed 50g almond /day for 4 weeks. A control group maintained habitual diets over the same period.Plasma α-tocopherol/cholesterol ratios were not different between groups at baseline and were significantly elevated by almond intervention with 50g almond/day for four weeks (p<0.05). Plasma protein oxidation and nitrite levels were not different between groups whereas total, HDL and LDL cholesterols and triglycerides were significantly higher in healthy middle-aged and young men with CV risk factors but were not affected by almond intake. In the almond consuming groups, flow mediated dilatation (FMD) was improved and diastolic blood pressure was reduced significantly after 4 weeks, but systolic blood pressure was only reduced in healthy men.In conclusion, a short-term almond-enriched diet can increase plasma α-tocopherol and improve vascular function in asymptomatic healthy mean men aged between 20 and 70 without effect on plasma lipids or markers of oxidative stress.3

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“…Reduced and oxidised bovine serum albumin (BSA) was prepared for carbonyl standards and the enzyme linked immunosorbent assay (ELISA) was performed as described by Carty et al (2000) [33].…”

Section: Membranesmentioning

confidence: 99%

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

Ruskovska

Bennett

Brown

et al. 2014

Free Radical Research

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Chronically haemodialysed end-stage renal disease patients are at high risk of morbidity arising from complications of dialysis, the underlying pathology that has led to renal disease and the complex pathology of chronic kidney disease. Anaemia is commonplace and its origins are multifactorial, involving reduced renal erythropoietin production, accumulation of uremic toxins and an increase in erythrocyte fragility.Oxidative damage is a common risk factor in renal disease and its co-morbidities, and is known to cause erythrocyte fragility. Therefore we have investigated the hypothesis that specific erythrocyte membrane proteins are more oxidised in endstage renal disease patients and that vitamin C supplementation can ameliorate membrane protein oxidation.Eleven patients and fifteen control subjects were recruited to the study. Patients were supplemented with 2 x 500mg vitamin C per day for four weeks. Erythrocyte membrane proteins were prepared pre-and post-vitamin C supplementation for determination of protein oxidation. Total protein carbonyls were reduced by vitamin C supplementation but not by dialysis when investigated by enzyme linked immunosorbent assay. Using a western blot to detect oxidised proteins, one protein band, later identified as containing ankyrin, was found to be oxidised in patients but not controls and was reduced significantly by 60% in all patients after dialysis and by 20% after vitamin C treatment pre-dialysis. Ankyrin oxidation analysis may be useful in a stratified medicines approach as a possible marker to identify requirements for intervention in dialysis patients.

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The Effects of Vitamin C Supplementation on Protein Oxidation in Healthy Volunteers

Cited by 123 publications

References 21 publications

Role of dietary antioxidants in the prevention of in vivo oxidative DNA damage

Role of dietary antioxidants in the prevention of in vivo oxidative DNA damage

An almond-enriched diet increases plasma α-tocopherol and improves vascular function but does not affect oxidative stress markers or lipid levels

Ankyrin is the major oxidised protein in erythrocyte membranes from end-stage renal disease patients on chronic haemodialysis and oxidation is decreased by dialysis and vitamin C supplementation

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