Abstract. Vehicle vibration has direct impact on human riding comfort. Against the needs of vehicle man-machine interface design and analysis in the dynamic environment, this paper made the simulation and experimental study of human riding comfort of man-automobile system in dynamic environment. A man-automobile system biomechanics simulation model was constructed in the software system of ADAMS/View and LifeMOD. Through the simulation of the road roughness, different road surfaces, as the vibration exciting, were added to the man-automobile system to dynamically analysis and study the human vibration characteristics under various running states. On the SA30-S802/ST electromagnetic vibration table, human vibration response of manautomobile in dynamic environment was test. Research results show that the man-automobile system biomechanics simulation model can make the simulation and evaluation study of human riding comfort effectively and provide a efficient means for the design and analysis of vehicle human-machine interface under dynamic environment, and have important technical significance for evaluating rapidly the human riding comfort of man-machine system (automobile, tractor, tank and so on).