The Effects of Ventilation, Humidity, and Temperature on Bacterial Growth and Bacterial Genera Distribution

Abstract

Background: Bacteria are readily nourished in airtight environments with high humidity, such as storage cabinets, clothing closets, and corners, where ventilation is normally low and humidity is high. Objectives: We characterized the role of humidity and ventilation in bacterial growth and genus distribution at different temperatures (26 °C and 34 °C). Methods: Fresh pork, which was used as the substrate for bacterial culture, was placed in storage cabinets. Bacterial growth and genera distribution on the surface of pork placed in a storage cabinet under different temperatures (26 °C and 34 °C); relative humidity levels (RH: 50%, 70%, 90%); and ventilation conditions (no ventilation and low, medium, and high levels of ventilation) were assessed by rDNA sequencing. Results: Increased ventilation and reduced humidity significantly decreased bacterial growth at 26 °C and 34 °C. The contribution of increased ventilation to the reduction in bacterial growth exceeded that of decreased humidity. Ventilation had the greatest effect on reducing bacterial growth compared to the unventilated conditions at 70% RH. At 34 °C, medium and high levels of ventilation were required to reduce bacterial growth. High temperatures greatly increased bacterial growth, but ventilation could reduce the degree of this increase.

Symbiotic Antimicrobial Effects of Cellulose‐Based Bio‐Nanocomposite for Disease Management of Agricultural Crops

“…It is due to the fact that high temperature can denature the enzymes and proteins carrying out essential bio‐prophecies. However, the effect of moisture can be understand in terms of humidity, increase in humidity increases the bacterial growth while decrease in humidity reduces the bacterial growth [48] . Similarly, the fungi fail to grow in the lack of moisture i. e., at low humidity [49] …”

“…However, the effect of moisture can be understand in terms of humidity, increase in humidity increases the bacterial growth while decrease in humidity reduces the bacterial growth. [48] Similarly, the fungi fail to grow in the lack of moisture i. e., at low humidity. [49] In the present work, all the experiments related to microbial activities were executed in the incubator providing controlled environment.…”

Metagenome-based microbial community analysis of urine-derived fertilizer

“…The dominance of Bacillota (Firmicutes) and Bacteroidota in the struvite sample is indicative of the fact that struvite is not conducive for all bacteria except those with resistance mechanisms such as spore formation. Struvite is characterized by its low water activity compared to the liquid fresh or stored urine (Qiu et al, 2022), which makes it less conducive for microbial thriving except for those with survival mechanisms such as dormancy (Wetzel and McBride, 2020). In the study by (Lahr et al, 2016), the genera Clostridia Tissierellia and Bacterodia which belong to the dominant phyla in our study were reported to persist in the struvite samples.…”

Reconciling airborne disease transmission concerns with energy saving requirements: the potential of UV-C pathogen deactivation and air distribution optimization

“…The air quality measurements carried out on Friday every week showed strong variations of both outdoor and indoor air quality between different weeks, with numbers of bacterial colony forming units (CFU) ranging typically between 10 and 1000 for outdoor air samples. This is not surprising since the presence of bacteria in the air is expected to depend on, e.g., the temperature and relative humidity [72,73]. Days of high outdoor bacterial concentration also showed high indoor bacterial concentration when the UVGI module was off.…”