The Effects of Vacuum Evaporation on Amino Acid Contents in Pureed Aloe Chinensis Baker Gel using HPLC

Abstract

Aloe Chinensis Baker is an aloe species that is frequently utilized in Asia, including Indonesia. Aloe vera contains various compounds in its leaves, such as vitamins, enzymes, and amino acids. The largest component of Aloe vera leaves is water. Vacuum evaporation is considered to be a potential method in the process to reduce the water content while maintaining the integrity of the nutrients. The objective of this research is to reduce the water content in pureed Aloe vera gel to obtain extracts. Evaporation using a rotary vacuum evaporator is conducted at an pressure of 110 mBar and temperature of 40 °C, and a varied time of 30, 60, 90, and 120 minutes. Analyses of amino acid contents are performed with HPLC. The results show that at 120 minutes, the preserved amino acid contents include: L-Isoleucine 29.3 ppm, L-Proline 10.8 ppm, L-Arginine 9.0 ppm, L-Asparagine 243 ppm, L-Threonine 56.9 ppm, L-Leucine 30.9 ppm, L-Methionine 38.4 ppm, L-Histidine 15.4 ppm, Aspartic Acid 24.4 ppm, L-Lysine 108.9 ppm, L-Cysteine 131.0 ppm, L-Alanine 40.2 ppm, and Glycine 230.7 ppm. The resulting Aloe vera extract show the following characteristics, density of 0.99 g/ml and pH of 6.5. Yield of 120 minutes evaporation is 40 %.

Chemical structures, biological activities, and medicinal potentials of amine compounds detected from Aloe species

“…Although there are a number of methods to detect amino acids, many of the amino acids (Table 3) detected in Aloe species were using an amino acid analyzer equipped with an HPLC and UV detector (Kim et al, 2013;Chandra Sekhar Singh et al, 2018). In addition to that, HPLC and 1 H NMR were used for the detection of amino acids in Aloe species (Hendrawati et al, 2019;Martínez-Sánchez et al, 2020). The analyzed amino acids from Aloe species are the twenty standard α-amino acids and their derivatives, which differ only in side chain (R) groups (Figure 7: structures 38-60).…”

Effect of natural precursors and micro/macroparticles addition on the morphology modulation ofStreptomyces toxytriciniKD18 stimulates lipstatin productivity

“…As mentioned in the literature, Aloe vera pulp, HLs and HFs are rich sources of leucine (an important component for lipstatin biosynthesis) isoleucine, phenylalanine and many other amino acids. 21,22 Supplementation of these components to the Streptomyces culture proved was not certified by peer review) is the author/funder. All rights reserved.…”

Characterization and Properties of Gedi (Abelmoschus Manihot L.) Leaf Extract with Liquid Chromatography Mass Spectrometry Using Quadrupole Time-of-Flight Technology (LCMS-QToF)

“…Gedi (Abelmoschus Manihot L.) is a tropical plant of the Malvaceae family and is classified as a medicinal herbal plant [1][2][3]. A. Manihot may be used as an alternative medicine to lower blood glucose levels and blood pressure and to act as an anti-inflammatory, antioxidant and antidepressant [4][5][6][7][8]. It is known to contain flavonoids, steroids, tannins and phenols.…”