Characterization and Properties of Gedi (Abelmoschus Manihot L.) Leaf Extract with Liquid Chromatography Mass Spectrometry Using Quadrupole Time-of-Flight Technology (LCMS-QToF)

Abstract

Gedi (Abelmoschus Manihot L.) is a tropical plant in the Malvaceae family, one of the groups of plants in the herbal community. Gedi plants are known to contain flavonoids, hormones, alkaloids, tannins and phenolic compounds. An ultrasonic extraction tool is used in the extraction process of Gedi leaves. The objective of the study is to know the yield and analyze Gedi leaf extract, including proximate analysis and active substance components with LCMS-QTof. The material used is Gedi leaves from Manado. Ultrasonic extraction was carried out with a dry Gedi leaf ratio and Ethanol 1:40 (m/v) with a time of 30 minutes. The concentration of the extract is with a rotary evaporator temperature of 50-60°C. The yield of the extract of Gedi leaf is 9.86 %. Study of the proximity of dried Gedi leaf powder revealed 27.135% protein, 41.675% carbohydrate, 329 kcal energy, 11.235% water and 13.83% ash material. Meanwhile for proximity products, the Gedi leaf extract contains 0.635% protein, 1.27% carbohydrate, 9.26% total energy, 7.1% water, and 0.635% ash. The active compounds in the Gedi extract have been analyzed using LCMS. Trigonelline, Gentiatibetine, Harman, Periplocoside C, and Biotin were detected using the positive-ionization mode, whereas Chloramphenicol was detected with negative-ionization mode.

Extraction and In Vitro Skincare Effect Assessment of Polysaccharides Extract from the Roots of Abelmoschus manihot (L.)

“…Given the lack of prior reports on the proximate composition of A. manihot root, the composition of A. manihot leaves serves as a comparison. A. manihot leaves showed contents of 13.83% ash content, 27.14% protein and 41.68% carbohydrates [28], while the roots exhibit a higher ash content (18.34 ± 0.45%) and lower protein (10.31 ± 0.68%) and carbohydrates content (7.86 ± 0.61%) than the leaves (Table 1). On one hand, roots primally absorb minerals from the soil, thus the inorganic substances, which are the major components of ash, are commonly higher in roots than in leaves [29].…”

PENENTUAN PROFIL KROMATOGRAFI LAPIS TIPIS, KADAR FENOLIK TOTAL, DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN GEDI (Abelmochus manihot L.)

“…Gedi dapat digunakan sebagai tanaman alternatif untuk menurunkan kadar glukosa darah, menurunkan tekanan darah, bersifat antiinflamasi, antioksidan, dan antidepresan. Gedi diketahui mengandung senyawa flavonoid, steroid, tanin, dan fenolik (Hendrawati et al, 2020). Fenolik merupakan metabolit sekuder yang umumnya yang terlibat dalam pertahanan terhadap radiasi sinar ultraviolet atau terhadap agresi patogen (Hano & Tungmunnithum, 2020).…”

Ultrasonic Extraction of Java Ginseng Leaves (Talinum Triangulare (Jacq.) Willd) with Variables of Ethanol Solution Ratio and Extraction Time

“…The extraction method that is mostly used for the process of taking active compounds from plants is maceration extraction (soaking). However, this method is known to be a conventional method and is considered to take quite a long time so it is considered less effective [5,3].…”

“…Phytochemical tests were carried out to determine secondary metabolites from plants. Secondary metabolites in plants include saponins, flavonoids, phenols, alkaloids, steroids, terpenoids and tannins [3]. The following is a description of the phytochemical properties of Javanese ginseng leaves [4].…”