The effects of turmeric (curcumin) on tumor suppressor protein (p53) and estrogen receptor (ERα) in breast cancer cells

Abstract

Curcumin (CUR) is a compound that has antibacterial, antiviral, anti-inflammatory, and anticancer properties. In this study, we have analyzed the effects of CUR on the expression of ERα and p53 in the presence of hormones and anti-hormones in breast cancer cells. Cells were cultured in a medium containing charcoal-stripped fetal bovine serum to deplete any endogenous steroids and treated with CUR at varying concentrations or in combination with hormones and anti-hormones. Protein analysis revealed a relative decrease in the levels of p53 and ERα upon treatment with 5–60 µM CUR. In cell proliferation studies, CUR alone caused a 10-fold decrease compared with the treatment with estrogen, which suggests its antiproliferative effects. Delineating the role of CUR in the regulation of p53, ERα, and their mechanisms of action may be important in understanding the influence of CUR on tumor suppressors and hormone receptors in breast cancer.

Curcumin ameliorates severe influenza pneumonia via attenuating lung injury and regulating macrophage cytokines production

“…A virus suspension supplemented with TPCK‐modified trypsin at 0.5 μg/mL was added at 1 infection multiplicity. Next, different curcumin doses (0, 0.1, 0.3, 1, 3 and 10 μmol/L of final concentrations, <10 μmol/L has a survival rate >60% for 6 days) were added to the cells at 24 hours post infection. After 96 hours post infection, 4% PFA was applied to A549 cells for fixation, then permeabilization was performed in PBS with 0.5% Triton X‐100 (AMRESCO, Solon, OH, USA), next PBS containing 5% BSA was used for blocking (Sigma).…”

The effects of black cohosh on the regulation of estrogen receptor (ER&alpha;) and progesterone receptor (PR) in breast cancer cells

“…These cells were routinely cultured following the same protocol as previous studies in our laboratory. 15 , 17 – 19 Cells were incubated at 37°C in an incubator with 5% CO 2 in RPMI-1640 media (Hyclone, Logan, UT, USA) and 10% fetal bovine serum (FBS; Hyclone) that contain growth factors and exogenous steroids which assist in cell growth and proliferation. The medium was replaced every 48 hours.…”

Black tea and curcumin synergistically mitigate the hepatotoxicity and nephropathic changes induced by chronic exposure to aflatoxin‐B1 in Sprague–Dawley rats

“…(2013) reported a significant antioxidant effect of curcumin (CMN) against the oxidative stress byproducts. CMN was suggested to have specific cellular pathways for AFB1 detoxification (Muhammad et al, 2018) and p53 downregulation in various tumors like colon (Moos, Edes, Mullally, & Fitzpatrick, 2004) and breast (Hallman et al., 2017) cancers, but scarce data are available for its role in liver or kidney cancers. The mostly known action of CMN is its role in restricting the aflatoxigenic potential of A. flavus , by inhibiting the cytochrome P450 isoenzymes, particularly CYP2A6 isoform; thereby reducing the formation of AFB1‐8,9‐epoxide and other aflatoxin metabolites (Limaye, Yu, Chou, Liu, & Cheng, 2018).…”