The effects of topical application of red pomegranate (Punica granatum Linn) extract gel on the healing process of traumatic ulcers in Wistar rats

Abstract

Background: Trauma-induced ulcers constitute one of the painful abnormalities affecting the oral cavity about which numerous individuals complain. The prevalence of traumatic ulcers (15-30% of cases) is relatively high compared to other oral lesions. Unfortunately, the use of anti-inflammatory drugs classified as steroids can lead to contraindications and serious side effects. Hence, the use of natural ingredients represents an alternative treatment. One such ingredient is red pomegranate containing bioactive elements acting as anti-inflammatory, antibacterial, antimicrobial, and antioxidant agents which accelerate the healing process in traumatic ulcers. Purpose: The aim of this study was to determine the effects of the topical application of red pomegranate (Punica granatum Linn) extract on the healing process in traumatic ulcers in Wistar rats. Methods: 24 male rats, aged 2-3 months, were divided into six groups, namely; two control groups (PC: triamcinolone acetonide and NC: sterile aquades) and four treatment groups (red pomegranate extract gel at respective concentrations of 12.5%, 25%, 50% and 75%). A traumatic ulcer was subsequently created using a flame-shaped burnisher tip 2mm in diameter and heated for ±15 seconds. The diameter traumatic ulcer of each research subject was measured and observed until it healed within the remedial parameters determined by means of a UNC-15 periodontal probe which had been placed on it. The data obtained was analyzed using Kruskal-Wallis and Mann-Whitney tests. Results: Topically applied red pomegranate extract gel can reduce the width of the ulcerated area, while also accelerating traumatic ulcer healing. There was a significant difference in the healing time between the group using red pomegranate extract gel at a concentration of 75% and the positive control group using 0.1% triamcinolone acetonide with p=0. 44 (p<0.05). Conclusion: Red pomegranate extract gel possesses the ability to accelerate the healing process in traumatic ulcers. The most effective concentration of red pomegranate extract gel at accelerating the healing process in traumatic ulcers is 75%.

<strong>Effectiveness of natural lip balm cinnamon <em>(Cinnamomum burmannii)</em> in accelerating the incision wound healing process in <em> rattus norvegicus </em> </strong>

“…22 Clinical studies performed by several researchers have demonstrated the application of medicinal plants to accelerate the healing process with antiinflammatory effects by their bioactivity abilities to promote wound healing and prevent infection without grave side effects. 23,24,25,26 According to another study, cinnamaldehyde effectively inhibited ox-LDL-induced vascular smooth muscle cells (VSMCs) proliferation, migration, and inflammatory cytokine overproduction, as well as foam cell formation in VSMCs and macrophages. 27 It has been demonstrated in several research with other animals that epithelialization increases.…”

Oral Ulcer Healing Activity of Lemon Pepper Ethanol Extract Gel in Streptozotocin-Induced Diabetic Wistar Rats

“…Moreover, the lemon pepper gel 5%, 7.5%, and 10% were made by mixing 2.5 grams, 3.75 grams, and 5 grams lemon pepper ethanol extract into 50 grams gel base, respectively. 2 The observations of ulcer size were performed immediately after the injury, 7 th , and 14 th days after the ulcer creation. The ulcer size was measured by vernier caliper in milimeter.…”

“…It usually takes 7 to 14 days for traumatic ulcers in the mouth to heal. 2 The objectives of ulcer therapy are to reduce the pain and promote healing, which can be achieved by eliminating the risk factor and applying topical ointments such as topical corticosteroids as the first line of ulcer therapy because they are inexpensive efficacious safe. Inflammatory response, cell proliferation, and production of the extracellular matrix components and the post-healing period, known as remodeling, are the three stages of cell and biochemical processes that are closely linked in wound repair.…”

The potential of liquid smoke as an oral ulcer remedies: A proposed mechanism based on systematic review

“…Systemic therapy, such as amlexanox and corticosteroids, are usually administered orally (Lewis and Wilson, 2019). However, the mechanism of action of amlexanox is still unknown, and long-term use of corticosteroids has side effects on other organs, such as the liver and the digestive tract, and may increase the incidence of Candida infection in the oral cavity (Abbasi et al, 2016;Hernawati et al, 2019;Lewis and Wilson, 2019).…”