The effects of tetracycline-loaded silk fibroin membrane on proliferation and osteogenic potential of mesenchymal stem cells

Jin, Seong-Ho; Kweon, HaeYong; Park, Jun‐Beom; Kim, Chang-Hyen

doi:10.1016/j.jss.2014.08.054

Cited by 22 publications

(25 citation statements)

References 43 publications

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“…Mesenchymal stem cells may be isolated from various tissues including gingival, as used in this study [16][17][18][19][20]. Gingiva can be easily accessible and obtainable [10,[21][22][23][24][25]. This procedure can be performed under local anesthesia and the obtained tissues are usually discarded in routine periodontal surgery [11,[26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Lee¹,

Uddin²,

Lee³

et al. 2018

biomedicalresearch

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Bambusa tulda has been used for various Purpose and is considerd as one of the most useful bamboo species. This study aimed to evaluate the effects of Bambusa tulda extract (BBT) on the osteogenic differentiation and mineralization of human mesenchymal stem cells. Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of BBT at concentrations ranging from 0.001% to 1%. Evaluations of cell morphology and cellular viability were done at days 1, 3, 5 and 7. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. The morphology of stem cells in the presence of BBT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not show any noticeable changes when compared with the untreated control group. The treatment of BBT (from 0.001% to 1% groups) showed decrease in alkaline phosphatase activity. The results of the Alizarin Red S staining showed a significant decrease with application of BBT. Conclusively, Bambusa tulda had influenced the osteogenic differentiation of the stem cells derived from the gingiva. Thus, the use of Bambusa tulda may produce adverse effects onto oral tissues. The concentration and application time of Bambusa tulda should be meticulously controlled to minimize the adverse effects.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Lee¹,

Uddin²,

Lee³

et al. 2018

biomedicalresearch

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“…This structure had shown good antibacterial activity and appropriate cell affinity [196]. Jin et al also observed that loading 1% or 5% tetracycline on SF constructs can enhance the proliferation rate and osteogenic potential of human gingiva-derived mesenchymal stem cells compared to those containing 10% tetracycline [197]. Finally, it is worthy to note that an effective delivery system must have some requirement including biocompatibility, biodegradability, durable mechanically support and the ability to be prepared under ambient aqueous environments in order to maintain the bioactivity of the delivered biomolecule.…”

Section: Figurementioning

confidence: 98%

Silk as a potential candidate for bone tissue engineering

Mottaghitalab

Hosseinkhani

Shokrgozar

et al. 2015

Journal of Controlled Release

135

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“…Human mesenchymal stem cells have been isolated and characterized from the periodontium including the gingiva (8), and gingiva-derived stem cells have been utilized for tissue-engineering purposes (9). Gingiva-derived stem cells from the maxillofacial region may be considered a favorable source of mesenchymal stem cells as harvesting stem cells from the mandible or maxilla may be easily performed under local anesthesia (8,10).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids

Lee¹,

Ko²,

Park³

2017

Experimental and Therapeutic Medicine

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Gingiva-derived stem cells have been applied for tissue-engineering purposes and may be considered a favorable source of mesenchymal stem cells as harvesting stem cells from the mandible or maxilla may be performed with ease under local anesthesia. The present study was performed to fabricate stem-cell spheroids using concave microwells and to evaluate the maintenance of stemness, viability, and differentiation potential. Gingiva-derived stem cells were isolated, and the stem cells of 4×105 (group A) or 8×105 (group B) cells were seeded into polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres and the change of the diameters of the spheroids were evaluated. The viability of spheroids was qualitatively analyzed via Live/Dead kit assay. A cell viability analysis was performed on days 1, 3, 6, and 12 with Cell Counting Kit-8. The maintenance of stemness was evaluated with immunocytochemical staining using SSEA-4, TRA-1-60(R) (positive markers), and SSEA-1 (negative marker). Osteogenic, adipogenic, and chondrogenic differentiation potential was evaluated by incubating spheroids in osteogenic, adipogenic and chondrogenic induction medium, respectively. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A than in group B. The majority of cells in the spheroids emitted green fluorescence, indicating the presence of live cells at day 6. At day 12, the majority of cells in the spheroids emitted green fluorescence, and a small portion of red fluorescence was also noted, which indicated the presence of dead cells. The spheroids were positive for the stem-cell markers SSEA-4 and TRA-1-60(R) and were negative for SSEA-1, suggesting that these spheroids primarily contained undifferentiated human stem cells. Osteogenic, adipogenic, and chondrogenic differentiation was more evident with an increase of incubation time: Mineralized extracellular deposits were observed following Alizarin Red S staining at days 14 and 21; oil globules were increased at day 18 when compared with day 6; and Alcian blue staining was more evident at day 18 when compared with day 6. Within the limits of this study, stem-cell spheroids from gingival cells maintained the stemness, viability, and differentiation potential during the experimental periods. This method may be applied for a promising strategy for stem-cell therapy.

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The effects of tetracycline-loaded silk fibroin membrane on proliferation and osteogenic potential of mesenchymal stem cells

Cited by 22 publications

References 43 publications

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Silk as a potential candidate for bone tissue engineering

Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids

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