The effects of temperature and pressure on the performance of Carboxen/PDMS fibres during solid phase microextraction (SPME) of headspace volatiles from cooked and raw turkey breast

Abstract

The strongly adsorptive SPME fibre Carboxen/polydimethylsiloxane (CAR/PDMS) was evaluated for its performance in the recovery of aroma-contributing compounds from the headspace of heated and raw homogenates of turkey breast muscle. In heated samples, variables such as fibre and sample temperatures, sample volume, headspace: sample ratio, and the presence or absence of internal headspace pressure significantly influenced the total amounts of aroma components recovered, as well as the proportions of headspace fractions, which differed in volatility. The versatile nature of the fibre permitted the selection of operating parameters that substantially increased the sensitivity of detection for minor components of both high and low volatility. Headspace sampling of weakly aromatic raw turkey breast homogenates and of aqueous standards at ambient temperature and a pressure of approximately 5 ð 10 2 bar resulted in a 4-70-fold increase in the recovery of aldehyde and alcohol headspace components, compared to the levels detected at atmospheric pressure. At reduced pressure the trapping ability of the CAR/PDMS fibre was found to be much superior to that of porous polymer materials such as PDMS/DVB, and the fibre has excellent potential as a simple and effective means of concentrating volatile components from raw foods and other matrices where the application of heat is not desired.

Volatile constituents of benzoin gums: Siam and Sumatra, part 2. Study of headspace sampling methods

“…The method, developed by Arthur and Pawliszyn 17 in 1990, is used in many applications: analysis of pollutants in water, headspace analysis of aromatic and medicinal plants 18 and food flavour analysis. 19 In a few years, SPME has considerably extended its applications in many fields.…”

Volatile constituents of benzoin gums: Siam and Sumatra. Part 1

“…16 In this work, we have selected two fibres: carboxen/ polydimethylsiloxane (CAR/PDMS) and carbowax/divinylbenzene (CW/DVB). CAR/PDMS fibre is recommended for low molecular weight compounds 17 and has been described as the best fibre to characterize volatile compounds such as menthol. 18 The mixed coating CW/ DVB was tried, as it was considered suitable for the analysis of polar semivolatiles.…”

Solid phase microextraction - gas chromatography for the evaluation of secondary lipid oxidation products in chicken patties during long-term storage

“…Different parameters were tested to optimize the method: weight of the sample (0.5, 1 and 2 g), water addition (0, 3 and 6 mL), addition of butylated hydroxyanisole (BHA) (0, 5 and 10 mg), time of fiber exposure in the headspace (5,10,15,20,30,40, and 60 min) and temperature of extraction (60 ºC and 80 ºC). Before analysis, the fibers were preconditioned in the GC injection port as indicated by the manufacturer.…”

“…Solid phase microextraction (SPME) is a solvent free sampling technique introduced in the 1990s by Arthur and Pawliszyn 1 and has successfully been used in the determination of volatile profile of foodstuffs [2][3][4][5][6] and also to follow the extent of lipid oxidation by the measure of secondary products, mainly in meat and meat products. [7][8][9][10][11][12] Although there are some limitations of interfiber comparisons, SPME technique was successfully applied for extended time-course experiments for monitoring volatile compounds in olive oil.…”