The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis

Lím, D.; Jang, Jae-Yeon; Kim, SeungJae; Kang, Taegyeong; Lee, Kangtaek; Choi, In Hong

doi:10.1016/j.biomaterials.2012.03.006

Cited by 95 publications

(79 citation statements)

References 30 publications

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“…As far as can be surmised, no studies to date have addressed the effects of pesticides on mouse macrophage lysosomal enzyme activity. Given the key role of macrophages in phagocytosis (Mosser & Edwards 2008), persistence of particles (Geiser 2010), and innate immunity (Lim et al 2012), CPF-and CBZ-induced inhibition of macrophage lysosomal enzyme activity could be considered a potential immunosuppressive mechanism by which CPF and CBZ pesticides exert immunotoxic effects.…”

Section: Discussionmentioning

confidence: 99%

Modulation of macrophage functionality induced in vitro by chlorpyrifos and carbendazim pesticides

Helali

Ferchichi

Maaouia

et al. 2016

Journal of Immunotoxicology

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The immune response is the first defense against pathogens; however, it is very sensitive and can be impacted on by agrochemicals such as carbamate and organophosphate pesticides widely present in the environment. To understand how pesticides can affect immune cell function in vitro, this study investigated the effects of chlorpyrifos (CPF) and carbendazim (CBZ), the most commonly used pesticides worldwide, on murine immune cell (i.e. macrophage) functions, including lysosomal enzyme activity and proinflammatory cytokines (IL-1b and TNFa) and nitric oxide (NO) production by isolated mouse peritoneal macrophages. This study showed for the first time that CPF and CBZ dose-relatedly reduced macrophage lysosomal enzyme activity and LPS-induced production of IL-1b, TNFa and NO. In general, the effects caused by CPF appeared more pronounced than those by CBZ. Collectively, these results demonstrated that CPF and CBZ exhibited marked immunomodulatory effects and could act as potent immunosuppressive factors in vitro. This inhibition of macrophage pro-inflammatory function may be an integral part of the underlying mode of action related to pesticide-induced immunosuppression.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Modulation of macrophage functionality induced in vitro by chlorpyrifos and carbendazim pesticides

Helali

Ferchichi

Maaouia

et al. 2016

Journal of Immunotoxicology

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“…Thus, consumers and workers may be exposed to AgNPs, which may pose harmful effects on their health [26]. Exposure to silver nanoparticles have been shown to induce liver toxicity including increase of cholesterol and alkaline phosphatase and bile duct hyperplasia in 28-day oral toxicity study [12][13][14] and 90-day oral subchronic study [14] and a 90-day inhalation studies [15].…”

Section: Discussionmentioning

confidence: 99%

Toxicogenomic Analysis for Livers from Sprague-Daley Rats Following 12-Week Inhalation Exposure to Silver Nanoparticles

2016

JNMR

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Silver nanoparticles (AgNPs) have been extensively applied to many industrial and biomedical fields due to their antibacterial effect. However, a large number of applications is also lead to health and environmental safety concerns. Up to date, it was well-known that AgNPs induced reactive oxygen species (ROS) production, cytotoxicity, pro-inflammatory effect, DNA damage, cell cycle disturb, necrosis and apoptosis by many researches. Also, several studies have been performed to investigate the microarray test for AgNPs in many cell types. However, no work reports the AgNPs toxicogenomic study in liver cell line and tissue until now. For this reason, we performed to in vivo toxicogenomic study for AgNPs inhalation exposed liver tissue. After 12 weeks inhalation exposure to AgNPs for the Sprague-Daley rats, we carried out silver concentration measurement for liver tissues and toxicogenomic analysis. As a result, we found that silver concentrations in livers were dose-dependently increased in male and female rats. However, a gender-different accumulation of silver in the livers did not observe. In toxicogenomic study, we observed that 109 and 150 genes significantly up-and down regulated by AgNPs inhalation exposure in male and female rats, respectively. The significantly altered male rat genes were involved in 54 biological pathways which were typically related with diabetes and metabolism. In female rat, the significantly expression changed genes were involved in 89 biological pathways which were mainly connected with metabolism and cell signaling. Plus, the gender-dependent gene expression changes of more than 2 fold were linked to 240 genes, with 114 genes in the male livers and 126 genes in the female livers. These were related to steroids and xenobiotics metabolism pathway.

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“…21 Briefly, the average size of AgNPs determined using transmission electron microscopy (model JEM-1011, JEOL, Tokyo, Japan) was 7. …”

Section: Materials and Methods Chemicalsmentioning

confidence: 99%

Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

Lee

Lee²,

Yun³

et al. 2015

IJN

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The silver nanoparticle (AgNP) is a candidate for anticancer therapy because of its effects on cell survival and signaling. Although numerous reports are available regarding their effect on cell death, the effect of AgNPs on metabolism is not well understood. In this study, we investigated the effect of AgNPs on glucose metabolism in hepatoma cell lines. Lactate release from both HepG2 and Huh7 cells was reduced with 5 nm AgNPs as early as 1 hour after treatment, when cell death did not occur. Treatment with 5 nm AgNPs decreased glucose consumption in HepG2 cells but not in Huh7 cells. Treatment with 5 nm AgNPs reduced nuclear factor erythroid 2-like 2 expression in both cell types without affecting its activation at the early time points after AgNPs’ treatment. Increased reactive oxygen species (ROS) production was detected 1 hour after 5 nm AgNPs’ treatment, and lactate release was restored in the presence of an ROS scavenger. Our results suggest that 5 nm AgNPs affect glucose metabolism by producing ROS.

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The effects of sub-lethal concentrations of silver nanoparticles on inflammatory and stress genes in human macrophages using cDNA microarray analysis

Cited by 95 publications

References 30 publications

Modulation of macrophage functionality induced in vitro by chlorpyrifos and carbendazim pesticides

Modulation of macrophage functionality induced in vitro by chlorpyrifos and carbendazim pesticides

Toxicogenomic Analysis for Livers from Sprague-Daley Rats Following 12-Week Inhalation Exposure to Silver Nanoparticles

Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

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