Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

Lee, Mi Jin; Lee, Seung Jun; Yun, Su Jin; Jang, Jae-Yeon; Kang, Hangoo; Kim, Kyongmin; Choi, In Hong; Park, Sun

doi:10.2147/ijn.s94907

Cited by 29 publications

(6 citation statements)

References 37 publications

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“…Furthermore, our results indicate that mitochondrial fission might be triggered by increased ROS generation due to MNPs@SiO 2 (RITC), and as a consequence, reduced ATP production and increased lactate concentration in MNPs@SiO 2 (RITC)-treated cells. Similar findings have been reported in Ag NPs-treated hepatoma cells [ 78 ].…”

Section: Discussionsupporting

confidence: 90%

Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics

et al. 2021

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Background Nanoparticles have been utilized in brain research and therapeutics, including imaging, diagnosis, and drug delivery, owing to their versatile properties compared to bulk materials. However, exposure to nanoparticles leads to their accumulation in the brain, but drug development to counteract this nanotoxicity remains challenging. To date, concerns have risen about the potential toxicity to the brain associated with nanoparticles exposure via penetration of the brain blood barrier to address this issue. Methods Here the effect of silica-coated-magnetic nanoparticles containing the rhodamine B isothiocyanate dye [MNPs@SiO2(RITC)] were assessed on microglia through toxicological investigation, including biological analysis and integration of transcriptomics, proteomics, and metabolomics. MNPs@SiO2(RITC)-induced biological changes, such as morphology, generation of reactive oxygen species, intracellular accumulation of MNPs@SiO2(RITC) using transmission electron microscopy, and glucose uptake efficiency, were analyzed in BV2 murine microglial cells. Each omics data was collected via RNA-sequencing-based transcriptome analysis, liquid chromatography-tandem mass spectrometry-based proteome analysis, and gas chromatography- tandem mass spectrometry-based metabolome analysis. The three omics datasets were integrated and generated as a single network using a machine learning algorithm. Nineteen compounds were screened and predicted their effects on nanotoxicity within the triple-omics network. Results Intracellular reactive oxygen species production, an inflammatory response, and morphological activation of cells were greater, but glucose uptake was lower in MNPs@SiO2(RITC)-treated BV2 microglia and primary rat microglia in a dose-dependent manner. Expression of 121 genes (from 41,214 identified genes), and levels of 45 proteins (from 5918 identified proteins) and 17 metabolites (from 47 identified metabolites) related to the above phenomena changed in MNPs@SiO2(RITC)-treated microglia. A combination of glutathione and citrate attenuated nanotoxicity induced by MNPs@SiO2(RITC) and ten other nanoparticles in vitro and in the murine brain, protecting mostly the hippocampus and thalamus. Conclusions Combination of glutathione and citrate can be one of the candidates for nanotoxicity alleviating drug against MNPs@SiO2(RITC) induced detrimental effect, including elevation of intracellular reactive oxygen species level, activation of microglia, and reduction in glucose uptake efficiency. In addition, our findings indicate that an integrated triple omics approach provides useful and sensitive toxicological assessment for nanoparticles and screening of drug for nanotoxicity. Graphical Abstract

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Section: Discussionsupporting

confidence: 90%

Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics

et al. 2021

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“…According to previous investigations 47 , 48 , silver nanoparticles (AgNPs) exhibit important antimicrobial activity 49 and could also be used as potential anticancer therapeutic agents 50 , 51 because of their intrinsic therapeutic properties. In cancer therapy, anti-proliferative effects of AgNPs might result from different underlying mechanisms, including induction of apoptosis 52 - 54 , production of ROS 55 , inhibitory action on the efflux activity of drug-resistant cells 56 , and reactivity with glutathione (GSH) molecules 57 .…”

Section: Metal-based Nanoenhancers For Ionizing Ramentioning

confidence: 99%

Metal-based NanoEnhancers for Future Radiotherapy: Radiosensitizing and Synergistic Effects on Tumor Cells

et al. 2018

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Radiotherapy is one of the major therapeutic strategies for cancer treatment. In the past decade, there has been growing interest in using high Z (atomic number) elements (materials) as radiosensitizers. New strategies in nanomedicine could help to improve cancer diagnosis and therapy at cellular and molecular levels. Metal-based nanoparticles usually exhibit chemical inertness in cellular and subcellular systems and may play a role in radiosensitization and synergistic cell-killing effects for radiation therapy. This review summarizes the efficacy of metal-based NanoEnhancers against cancers in both in vitro and in vivo systems for a range of ionizing radiations including gamma-rays, X-rays, and charged particles. The potential of translating preclinical studies on metal-based nanoparticles-enhanced radiation therapy into clinical practice is also discussed using examples of several metal-based NanoEnhancers (such as CYT-6091, AGuIX, and NBTXR3). Also, a few general examples of theranostic multimetallic nanocomposites are presented, and the related biological mechanisms are discussed.

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“…Estudos mostram que células tumorais de células epiteliais alveolares humanas (A549), células neuroendócrinas e células de ovário já foram encontradas em experimentos com prata 19 , além de já ter sido comprovada sua efetividade contra células de leucemia mieloide e câncer de mama 27 . Na Tabela 1, são apresentados alguns experimentos envolvendo NP de prata e seus respectivos índices de diminuição tumoral.…”

Section: Resultsunclassified

Utilização de Nanopartículas no Tratamento do Câncer: Aspectos Gerais, Mecanismos de Ação Antineoplásicos e Aplicabilidades Tumorais

Lopes¹,

Torres²

2020

Rev. Brasileira.De.Cancerologia

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Introdução: Os avanços tecnológicos das últimas décadas levaram ao desenvolvimento de abordagens terapêuticas na medicina em uma nova escala de aplicações moleculares nanométricas, permitindo o estabelecimento de novas terapias como a nanoterapia. O uso de nanopartículas implica inúmeras oportunidades e também grandes desafios para a aplicação humana, esse uso requer uma compreensão minunciosa da biocompatibilidade e da farmacodinâmica das moléculas. No câncer, as nanopartículas atuam principalmente na forma ativa, que se caracteriza pela utilização de ligantes ou anticorpos em suas superfícies onde se ligam a determinadas células, e, na passiva, onde as partículas se acumulam nos tecidos tumorais em razão das largas frenestrações dos endotélios tumorais. Objetivo: Abordar as principais propriedades das nanopartículas de proteína, metal, polímero e lipídio, suas estruturas e utilizações contra células cancerígenas específicas e as vantagens e desvantagens do seu uso. Método: Selecionar artigos com estudos sobre nanopartículas entre os anos de 2010 a 2018 nos bancos de dados PubMed e SciELO por meio de revisão integrativa da literatura. Resultados: As nanopartículas apresentam diferentes mecanismos de ação e podem obter resultados mais favoráveis de acordo com sua estrutura química. Conclusão: Concluiu-se com esta revisão que determinadas nanopartículas apresentam funcionalidades que potencializam os efeitos anticancerígenos dos fármacos antineoplásicos e surgem como uma nova abordagem para tratamentos contra o câncer.

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Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

Cited by 29 publications

References 37 publications

Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics

Silica-coated magnetic-nanoparticle-induced cytotoxicity is reduced in microglia by glutathione and citrate identified using integrated omics

Metal-based NanoEnhancers for Future Radiotherapy: Radiosensitizing and Synergistic Effects on Tumor Cells

Utilização de Nanopartículas no Tratamento do Câncer: Aspectos Gerais, Mecanismos de Ação Antineoplásicos e Aplicabilidades Tumorais

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